Association of Helicobacter pylori with colorectal cancer development.

Background: Helicobacter pylori (H. pylori) may be associated with colorectal cancer. However, the underlying mechanisms are still unclear. Objectives: Explore the serostatus of H. pylori cytotoxicity-associated gene A product (CagA) in patients with colorectal carcinoma, and assess the associatio...

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Main Authors:	Fouad Alsamaka, Fadi, Abdulamir, Ahmed Sahib, Khalid Mahdi, Laila, Alnaib, Khalid, Abu Bakar, Fatimah
Format:	Article
Language:	English English
Published:	IOS Press 2010
Online Access:	http://psasir.upm.edu.my/id/eprint/13314/ http://psasir.upm.edu.my/id/eprint/13314/1/Association%20of%20Helicobacter%20pylori%20with%20colorectal%20cancer%20development.pdf

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author	Fouad Alsamaka, Fadi Abdulamir, Ahmed Sahib Khalid Mahdi, Laila Alnaib, Khalid Abu Bakar, Fatimah
author_facet	Fouad Alsamaka, Fadi Abdulamir, Ahmed Sahib Khalid Mahdi, Laila Alnaib, Khalid Abu Bakar, Fatimah
author_sort	Fouad Alsamaka, Fadi
building	UPM Institutional Repository
collection	Online Access
description	Background: Helicobacter pylori (H. pylori) may be associated with colorectal cancer. However, the underlying mechanisms are still unclear. Objectives: Explore the serostatus of H. pylori cytotoxicity-associated gene A product (CagA) in patients with colorectal carcinoma, and assess the association of H. pylori with colorectal cancer via c-Myc and MUC-2 proteins at tumor tissues. Methods: H. pylori CagA IgG antibodies were screened using enzyme-linked immunosorbent assay (ELISA) in 30 patients with colorectal carcinoma and 30 cancer-free control subjects. Paraffin-embedded blocks were examined for the expression of c-Myc and MUC-2 protein by immunohistochemistry. Results: H. pylori CagA seropositivity increased significantly among colorectal cancer patients (p <0.05). The expression of c-Myc and MUC-2 in colorectal carcinoma patients was over-expressed (80%), and downexpressed (63%) in resection margins (p <0.05). c-Myc over-expression and MUC-2 down-expression were associated with CagA-positive rather than CagA-negative H. pylori patients. In 16 CagA seropositive vs. 14 CagA seronegative patients, the expression rate was 97.3% vs. 64.2% and 33.3% vs. 78.5% for cMyc and MUC-2, respectively. CagA IgG level was significantly higher in positive than in negative c-Myc patients (p= 0.036), and in negative than in positive MUC-2 patients (p= 0.044). c-Myc and MUC-2 were positively and inversely correlated with CagA IgG level (p <0.05). Conclusions: CagA-seropositive H. pylori is most probably associated with colorectal cancer development. Part of the underlying mechanism for such association might be via alterations in expression of MUC-2, which depletes the mucous protective layer in the colo-rectum, and c-Myc, which stimulates the growth of cancerous cells.
first_indexed	2025-11-15T07:53:25Z
format	Article
id	upm-13314
institution	Universiti Putra Malaysia
institution_category	Local University
language	English English
last_indexed	2025-11-15T07:53:25Z
publishDate	2010
publisher	IOS Press
recordtype	eprints
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spelling	upm-133142015-09-22T03:42:12Z http://psasir.upm.edu.my/id/eprint/13314/ Association of Helicobacter pylori with colorectal cancer development. Fouad Alsamaka, Fadi Abdulamir, Ahmed Sahib Khalid Mahdi, Laila Alnaib, Khalid Abu Bakar, Fatimah Background: Helicobacter pylori (H. pylori) may be associated with colorectal cancer. However, the underlying mechanisms are still unclear. Objectives: Explore the serostatus of H. pylori cytotoxicity-associated gene A product (CagA) in patients with colorectal carcinoma, and assess the association of H. pylori with colorectal cancer via c-Myc and MUC-2 proteins at tumor tissues. Methods: H. pylori CagA IgG antibodies were screened using enzyme-linked immunosorbent assay (ELISA) in 30 patients with colorectal carcinoma and 30 cancer-free control subjects. Paraffin-embedded blocks were examined for the expression of c-Myc and MUC-2 protein by immunohistochemistry. Results: H. pylori CagA seropositivity increased significantly among colorectal cancer patients (p <0.05). The expression of c-Myc and MUC-2 in colorectal carcinoma patients was over-expressed (80%), and downexpressed (63%) in resection margins (p <0.05). c-Myc over-expression and MUC-2 down-expression were associated with CagA-positive rather than CagA-negative H. pylori patients. In 16 CagA seropositive vs. 14 CagA seronegative patients, the expression rate was 97.3% vs. 64.2% and 33.3% vs. 78.5% for cMyc and MUC-2, respectively. CagA IgG level was significantly higher in positive than in negative c-Myc patients (p= 0.036), and in negative than in positive MUC-2 patients (p= 0.044). c-Myc and MUC-2 were positively and inversely correlated with CagA IgG level (p <0.05). Conclusions: CagA-seropositive H. pylori is most probably associated with colorectal cancer development. Part of the underlying mechanism for such association might be via alterations in expression of MUC-2, which depletes the mucous protective layer in the colo-rectum, and c-Myc, which stimulates the growth of cancerous cells. IOS Press 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13314/1/Association%20of%20Helicobacter%20pylori%20with%20colorectal%20cancer%20development.pdf Fouad Alsamaka, Fadi and Abdulamir, Ahmed Sahib and Khalid Mahdi, Laila and Alnaib, Khalid and Abu Bakar, Fatimah (2010) Association of Helicobacter pylori with colorectal cancer development. Asian Biomedicine, 4 (4). pp. 609-618. ISSN 1905-7415; ESSN:1875-855X English
spellingShingle	Fouad Alsamaka, Fadi Abdulamir, Ahmed Sahib Khalid Mahdi, Laila Alnaib, Khalid Abu Bakar, Fatimah Association of Helicobacter pylori with colorectal cancer development.
title	Association of Helicobacter pylori with colorectal cancer development.
title_full	Association of Helicobacter pylori with colorectal cancer development.
title_fullStr	Association of Helicobacter pylori with colorectal cancer development.
title_full_unstemmed	Association of Helicobacter pylori with colorectal cancer development.
title_short	Association of Helicobacter pylori with colorectal cancer development.
title_sort	association of helicobacter pylori with colorectal cancer development.
url	http://psasir.upm.edu.my/id/eprint/13314/ http://psasir.upm.edu.my/id/eprint/13314/1/Association%20of%20Helicobacter%20pylori%20with%20colorectal%20cancer%20development.pdf

Association of Helicobacter pylori with colorectal cancer development.

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