Association of G2350A polymorphism of angiotensin converting enzyme gene with essential hypertension and type 2 diabetes mellitus in Malaysian subjects.
The objective of this study was to determine the association of Angiotensin-Converting Enzyme (ACE) G2350A polymorphism in Essential Hypertension (EHT) and Type 2 Diabetes Mellitus (T2DM) in Malaysian subjects. A total of 265 samples have been recruited for this study. In order to amplify the 122 bp...
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| Format: | Article |
| Language: | English |
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Asian Network for Scientific Information
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13313/ http://psasir.upm.edu.my/id/eprint/13313/1/Association%20of%20G2350A%20polymorphism%20of%20angiotensin%20converting%20enzyme%20gene%20with%20essential%20hypertension%20and%20type%202%20diabetes%20mellitus%20in%20Malaysian%20subjects.pdf |
| _version_ | 1848842078885249024 |
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| author | Vasudevan, Ramachandran Ismail, Patimah Stanslas, Johnson Shamsudin, Norashikin |
| author_facet | Vasudevan, Ramachandran Ismail, Patimah Stanslas, Johnson Shamsudin, Norashikin |
| author_sort | Vasudevan, Ramachandran |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The objective of this study was to determine the association of Angiotensin-Converting Enzyme (ACE) G2350A polymorphism in Essential Hypertension (EHT) and Type 2 Diabetes Mellitus (T2DM) in Malaysian subjects. A total of 265 samples have been recruited for this study. In order to amplify the 122 bp of G2350A variant of ACE gene, genomic DNA was amplified using Hot-start Polymerase-Chain Reaction (PCR) and the PCR products was digested with BstuI restriction enzyme to produce 103 and 19 bp when A is at position 2350. The subjects were ranged from 30 to 84 years old witi mean age of 51.84 years. The frequency for GG, GA and AA genotypes of ACE gene was 41.43, 48.57 and 10% in EHT, 46.67, 36.67 and 16.66% in T2DM, 32.31, 53.85 and 13.84% in T2DM with EHT compared to 61.43, 35.71 and 2.86% of control subjects respectively. The frequency for A allele of G2350A polymorphism was 34.29% in EHT, 35.00% in T2DM, 40.77% in T2DM+EHT subjects compared to controls (20.71%). The genotype and allele frequency of ACE G2350A gene polymorphism differed significantly in patients when compared to controls (p<0.05). Allele A of G2350A polymorphism of ACE gene is associated with essential hypertension and Type 2 Diabetes Mellitus in Malaysian subjects. |
| first_indexed | 2025-11-15T07:53:25Z |
| format | Article |
| id | upm-13313 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:53:25Z |
| publishDate | 2008 |
| publisher | Asian Network for Scientific Information |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-133132015-10-08T02:42:53Z http://psasir.upm.edu.my/id/eprint/13313/ Association of G2350A polymorphism of angiotensin converting enzyme gene with essential hypertension and type 2 diabetes mellitus in Malaysian subjects. Vasudevan, Ramachandran Ismail, Patimah Stanslas, Johnson Shamsudin, Norashikin The objective of this study was to determine the association of Angiotensin-Converting Enzyme (ACE) G2350A polymorphism in Essential Hypertension (EHT) and Type 2 Diabetes Mellitus (T2DM) in Malaysian subjects. A total of 265 samples have been recruited for this study. In order to amplify the 122 bp of G2350A variant of ACE gene, genomic DNA was amplified using Hot-start Polymerase-Chain Reaction (PCR) and the PCR products was digested with BstuI restriction enzyme to produce 103 and 19 bp when A is at position 2350. The subjects were ranged from 30 to 84 years old witi mean age of 51.84 years. The frequency for GG, GA and AA genotypes of ACE gene was 41.43, 48.57 and 10% in EHT, 46.67, 36.67 and 16.66% in T2DM, 32.31, 53.85 and 13.84% in T2DM with EHT compared to 61.43, 35.71 and 2.86% of control subjects respectively. The frequency for A allele of G2350A polymorphism was 34.29% in EHT, 35.00% in T2DM, 40.77% in T2DM+EHT subjects compared to controls (20.71%). The genotype and allele frequency of ACE G2350A gene polymorphism differed significantly in patients when compared to controls (p<0.05). Allele A of G2350A polymorphism of ACE gene is associated with essential hypertension and Type 2 Diabetes Mellitus in Malaysian subjects. Asian Network for Scientific Information 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13313/1/Association%20of%20G2350A%20polymorphism%20of%20angiotensin%20converting%20enzyme%20gene%20with%20essential%20hypertension%20and%20type%202%20diabetes%20mellitus%20in%20Malaysian%20subjects.pdf Vasudevan, Ramachandran and Ismail, Patimah and Stanslas, Johnson and Shamsudin, Norashikin (2008) Association of G2350A polymorphism of angiotensin converting enzyme gene with essential hypertension and type 2 diabetes mellitus in Malaysian subjects. Journal of Biological Sciences, 8 (6). pp. 1045-1050. ISSN 1727-3048; ESSN: 1812-5719 http://scialert.net/abstract/?doi=jbs.2008.1045.1050 10.3923/jbs.2008.1045.1050 |
| spellingShingle | Vasudevan, Ramachandran Ismail, Patimah Stanslas, Johnson Shamsudin, Norashikin Association of G2350A polymorphism of angiotensin converting enzyme gene with essential hypertension and type 2 diabetes mellitus in Malaysian subjects. |
| title | Association of G2350A polymorphism of angiotensin converting enzyme gene with essential hypertension and type 2 diabetes mellitus in Malaysian subjects. |
| title_full | Association of G2350A polymorphism of angiotensin converting enzyme gene with essential hypertension and type 2 diabetes mellitus in Malaysian subjects. |
| title_fullStr | Association of G2350A polymorphism of angiotensin converting enzyme gene with essential hypertension and type 2 diabetes mellitus in Malaysian subjects. |
| title_full_unstemmed | Association of G2350A polymorphism of angiotensin converting enzyme gene with essential hypertension and type 2 diabetes mellitus in Malaysian subjects. |
| title_short | Association of G2350A polymorphism of angiotensin converting enzyme gene with essential hypertension and type 2 diabetes mellitus in Malaysian subjects. |
| title_sort | association of g2350a polymorphism of angiotensin converting enzyme gene with essential hypertension and type 2 diabetes mellitus in malaysian subjects. |
| url | http://psasir.upm.edu.my/id/eprint/13313/ http://psasir.upm.edu.my/id/eprint/13313/ http://psasir.upm.edu.my/id/eprint/13313/ http://psasir.upm.edu.my/id/eprint/13313/1/Association%20of%20G2350A%20polymorphism%20of%20angiotensin%20converting%20enzyme%20gene%20with%20essential%20hypertension%20and%20type%202%20diabetes%20mellitus%20in%20Malaysian%20subjects.pdf |