Assessment of Clarias batrachus as a source of acetylcholinesterase (AChE) for the detection of insecticides
An inhibitive assay of insecticides using Acetylcholinesterase (AChE) from the local fish Clarias batrachus is reported. AChE was assayed according to the modified method of Ellman. Screening of insecticide and heavy metals showed that carbofuran and carbaryl strongly inhibited C. batrachus AChE. Th...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Triveni Enterprises
2009
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/13300/ http://psasir.upm.edu.my/id/eprint/13300/2/Assessment%20of%20clarias%20batrachus%20as%20a%20source%20of%20acetylcholinesterase.pdf |
| _version_ | 1848842075316944896 |
|---|---|
| author | Tham, Lik Gin Perumal, Natarajan Syed, Mohd Arif Shamaan, Nor Aripin Abd. Shukor, Mohd. Yunus |
| author_facet | Tham, Lik Gin Perumal, Natarajan Syed, Mohd Arif Shamaan, Nor Aripin Abd. Shukor, Mohd. Yunus |
| author_sort | Tham, Lik Gin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An inhibitive assay of insecticides using Acetylcholinesterase (AChE) from the local fish Clarias batrachus is reported. AChE was assayed according to the modified method of Ellman. Screening of insecticide and heavy metals showed that carbofuran and carbaryl strongly inhibited C. batrachus AChE. The inhibition concentration (IC) IC50 values (and the 95% confidence interval) for both carbofuran and carbaryl inhibition on C. batrachus AChE at 6.66 (5.97-7.52) and 130.00 (119.3-142.5) mg l-1, respectively, was within the IC50 range of Electrophorus electricus at 6.20 (6.03-6.39) and 133.01 (122.40-145.50) mg l-1, respectively, and were much lower than bovine AChE at 20.94 (19.53-22.58) and 418.80 (390.60-451.60) mg l-1, respectively. The results showed that C. batrachus have the potential to be used as a cheaper and more readily available source of AChE than other more commercially available sources. |
| first_indexed | 2025-11-15T07:53:22Z |
| format | Article |
| id | upm-13300 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:53:22Z |
| publishDate | 2009 |
| publisher | Triveni Enterprises |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-133002018-03-27T07:55:32Z http://psasir.upm.edu.my/id/eprint/13300/ Assessment of Clarias batrachus as a source of acetylcholinesterase (AChE) for the detection of insecticides Tham, Lik Gin Perumal, Natarajan Syed, Mohd Arif Shamaan, Nor Aripin Abd. Shukor, Mohd. Yunus An inhibitive assay of insecticides using Acetylcholinesterase (AChE) from the local fish Clarias batrachus is reported. AChE was assayed according to the modified method of Ellman. Screening of insecticide and heavy metals showed that carbofuran and carbaryl strongly inhibited C. batrachus AChE. The inhibition concentration (IC) IC50 values (and the 95% confidence interval) for both carbofuran and carbaryl inhibition on C. batrachus AChE at 6.66 (5.97-7.52) and 130.00 (119.3-142.5) mg l-1, respectively, was within the IC50 range of Electrophorus electricus at 6.20 (6.03-6.39) and 133.01 (122.40-145.50) mg l-1, respectively, and were much lower than bovine AChE at 20.94 (19.53-22.58) and 418.80 (390.60-451.60) mg l-1, respectively. The results showed that C. batrachus have the potential to be used as a cheaper and more readily available source of AChE than other more commercially available sources. Triveni Enterprises 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13300/2/Assessment%20of%20clarias%20batrachus%20as%20a%20source%20of%20acetylcholinesterase.pdf Tham, Lik Gin and Perumal, Natarajan and Syed, Mohd Arif and Shamaan, Nor Aripin and Abd. Shukor, Mohd. Yunus (2009) Assessment of Clarias batrachus as a source of acetylcholinesterase (AChE) for the detection of insecticides. Journal of Environmental Biology, 30 (1). pp. 135-138. ISSN 0254-8704; ESSN: 2394-0379 http://www.jeb.co.in/index.php?page=issue_toc&issue=200901_jan09_spl |
| spellingShingle | Tham, Lik Gin Perumal, Natarajan Syed, Mohd Arif Shamaan, Nor Aripin Abd. Shukor, Mohd. Yunus Assessment of Clarias batrachus as a source of acetylcholinesterase (AChE) for the detection of insecticides |
| title | Assessment of Clarias batrachus as a source of acetylcholinesterase (AChE) for the detection of insecticides |
| title_full | Assessment of Clarias batrachus as a source of acetylcholinesterase (AChE) for the detection of insecticides |
| title_fullStr | Assessment of Clarias batrachus as a source of acetylcholinesterase (AChE) for the detection of insecticides |
| title_full_unstemmed | Assessment of Clarias batrachus as a source of acetylcholinesterase (AChE) for the detection of insecticides |
| title_short | Assessment of Clarias batrachus as a source of acetylcholinesterase (AChE) for the detection of insecticides |
| title_sort | assessment of clarias batrachus as a source of acetylcholinesterase (ache) for the detection of insecticides |
| url | http://psasir.upm.edu.my/id/eprint/13300/ http://psasir.upm.edu.my/id/eprint/13300/ http://psasir.upm.edu.my/id/eprint/13300/2/Assessment%20of%20clarias%20batrachus%20as%20a%20source%20of%20acetylcholinesterase.pdf |