Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of vitex trifolia linn possessing anti-tracheospasmolytic activity.
We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatog...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Taylor & Francis
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13246/ http://psasir.upm.edu.my/id/eprint/13246/1/Application%20of%20two%20dimensional%20thin%20layer%20chromatography%20pattern%20comparison%20for%20fingerprinting%20the%20active%20compounds%20in%20the%20leaves%20of%20Vitex%20trifolia%20Linn%20possessing%20anti.pdf |
| _version_ | 1848842060491128832 |
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| author | Khatib, Alfi Hoe, Arie C. Selamat, Jinap Sarker, Zaidul Islam Jaswir, Irwandi Verpoorte, Robert |
| author_facet | Khatib, Alfi Hoe, Arie C. Selamat, Jinap Sarker, Zaidul Islam Jaswir, Irwandi Verpoorte, Robert |
| author_sort | Khatib, Alfi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar/semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform/methanol (9/1) as the first mobile phase and ethyl acetate/chloroform/methanol (28/28/44) as the second mobile phase. |
| first_indexed | 2025-11-15T07:53:08Z |
| format | Article |
| id | upm-13246 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:53:08Z |
| publishDate | 2010 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-132462015-09-22T06:57:29Z http://psasir.upm.edu.my/id/eprint/13246/ Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of vitex trifolia linn possessing anti-tracheospasmolytic activity. Khatib, Alfi Hoe, Arie C. Selamat, Jinap Sarker, Zaidul Islam Jaswir, Irwandi Verpoorte, Robert We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar/semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform/methanol (9/1) as the first mobile phase and ethyl acetate/chloroform/methanol (28/28/44) as the second mobile phase. Taylor & Francis 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13246/1/Application%20of%20two%20dimensional%20thin%20layer%20chromatography%20pattern%20comparison%20for%20fingerprinting%20the%20active%20compounds%20in%20the%20leaves%20of%20Vitex%20trifolia%20Linn%20possessing%20anti.pdf Khatib, Alfi and Hoe, Arie C. and Selamat, Jinap and Sarker, Zaidul Islam and Jaswir, Irwandi and Verpoorte, Robert (2010) Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of vitex trifolia linn possessing anti-tracheospasmolytic activity. Journal of Liquid Chromatography and Related Technologies, 33 (2). pp. 214-224. ISSN 1082-6076; ESSN: 1520-572X 10.1080/10826070903439416 English |
| spellingShingle | Khatib, Alfi Hoe, Arie C. Selamat, Jinap Sarker, Zaidul Islam Jaswir, Irwandi Verpoorte, Robert Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of vitex trifolia linn possessing anti-tracheospasmolytic activity. |
| title | Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of vitex trifolia linn possessing anti-tracheospasmolytic activity. |
| title_full | Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of vitex trifolia linn possessing anti-tracheospasmolytic activity. |
| title_fullStr | Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of vitex trifolia linn possessing anti-tracheospasmolytic activity. |
| title_full_unstemmed | Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of vitex trifolia linn possessing anti-tracheospasmolytic activity. |
| title_short | Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of vitex trifolia linn possessing anti-tracheospasmolytic activity. |
| title_sort | application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of vitex trifolia linn possessing anti-tracheospasmolytic activity. |
| url | http://psasir.upm.edu.my/id/eprint/13246/ http://psasir.upm.edu.my/id/eprint/13246/ http://psasir.upm.edu.my/id/eprint/13246/1/Application%20of%20two%20dimensional%20thin%20layer%20chromatography%20pattern%20comparison%20for%20fingerprinting%20the%20active%20compounds%20in%20the%20leaves%20of%20Vitex%20trifolia%20Linn%20possessing%20anti.pdf |