Application of RT-PCR to detect treated and untreated Staphylococcus aureus genes with marine algae
Methacillin Resistant Staphylococcus aureus (MRSA), Extended Spectrum Beta Lactamase (ESBL) organisms and Multiple Drug Resistant Organism (MDRO). Therefore, this study was designed to explore an alternative antibacterial product derived from seaweed extracts, Gracilaria changii and Euchema denticul...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Medwell Journals
2009
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/13239/ http://psasir.upm.edu.my/id/eprint/13239/1/Application%20of%20RT.pdf |
| _version_ | 1848842058490445824 |
|---|---|
| author | Abdullah Al-Haj, Nagi Ahmed Mashan, Nurmas Idayu Shamsudin, Mariana Nor Mohamad, Habsah Vairappan, Charles S. Sekawi, Zamberi |
| author_facet | Abdullah Al-Haj, Nagi Ahmed Mashan, Nurmas Idayu Shamsudin, Mariana Nor Mohamad, Habsah Vairappan, Charles S. Sekawi, Zamberi |
| author_sort | Abdullah Al-Haj, Nagi Ahmed |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Methacillin Resistant Staphylococcus aureus (MRSA), Extended Spectrum Beta Lactamase (ESBL) organisms and Multiple Drug Resistant Organism (MDRO). Therefore, this study was designed to explore an alternative antibacterial product derived from seaweed extracts, Gracilaria changii and Euchema denticulatum, through the study of DNA and RNA encoding genes of interest in MRSA and non-MRSA. The target of this study is to amplification of several untreated and treated S. aureus and E. coli genes that are potentially involved in the antibacterial activities through RT-PCR assay. G. changii and E. denticulatum extracts showed inhibitory activity against S. aureus, several genes in this pathogen were chosen to study the effect of both seaweed extracts on the genes through PCR and RT-PCR analysis. However, the predicted inhibitory mechanism of both seaweeds extracts on mecA gene was not fully elucidated in the study. The investigation could scientifically proof the natural products to be potentially potent antibacterial agents. |
| first_indexed | 2025-11-15T07:53:06Z |
| format | Article |
| id | upm-13239 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:53:06Z |
| publishDate | 2009 |
| publisher | Medwell Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-132392017-09-28T09:18:59Z http://psasir.upm.edu.my/id/eprint/13239/ Application of RT-PCR to detect treated and untreated Staphylococcus aureus genes with marine algae Abdullah Al-Haj, Nagi Ahmed Mashan, Nurmas Idayu Shamsudin, Mariana Nor Mohamad, Habsah Vairappan, Charles S. Sekawi, Zamberi Methacillin Resistant Staphylococcus aureus (MRSA), Extended Spectrum Beta Lactamase (ESBL) organisms and Multiple Drug Resistant Organism (MDRO). Therefore, this study was designed to explore an alternative antibacterial product derived from seaweed extracts, Gracilaria changii and Euchema denticulatum, through the study of DNA and RNA encoding genes of interest in MRSA and non-MRSA. The target of this study is to amplification of several untreated and treated S. aureus and E. coli genes that are potentially involved in the antibacterial activities through RT-PCR assay. G. changii and E. denticulatum extracts showed inhibitory activity against S. aureus, several genes in this pathogen were chosen to study the effect of both seaweed extracts on the genes through PCR and RT-PCR analysis. However, the predicted inhibitory mechanism of both seaweeds extracts on mecA gene was not fully elucidated in the study. The investigation could scientifically proof the natural products to be potentially potent antibacterial agents. Medwell Journals 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13239/1/Application%20of%20RT.pdf Abdullah Al-Haj, Nagi Ahmed and Mashan, Nurmas Idayu and Shamsudin, Mariana Nor and Mohamad, Habsah and Vairappan, Charles S. and Sekawi, Zamberi (2009) Application of RT-PCR to detect treated and untreated Staphylococcus aureus genes with marine algae. Research Journal of Biological Sciences, 4 (8). pp. 952-959. ISSN 1815-8846; ESSN: 1993-6087 http://medwelljournals.com/abstract/?doi=rjbsci.2009.952.959 |
| spellingShingle | Abdullah Al-Haj, Nagi Ahmed Mashan, Nurmas Idayu Shamsudin, Mariana Nor Mohamad, Habsah Vairappan, Charles S. Sekawi, Zamberi Application of RT-PCR to detect treated and untreated Staphylococcus aureus genes with marine algae |
| title | Application of RT-PCR to detect treated and untreated Staphylococcus aureus genes with marine algae |
| title_full | Application of RT-PCR to detect treated and untreated Staphylococcus aureus genes with marine algae |
| title_fullStr | Application of RT-PCR to detect treated and untreated Staphylococcus aureus genes with marine algae |
| title_full_unstemmed | Application of RT-PCR to detect treated and untreated Staphylococcus aureus genes with marine algae |
| title_short | Application of RT-PCR to detect treated and untreated Staphylococcus aureus genes with marine algae |
| title_sort | application of rt-pcr to detect treated and untreated staphylococcus aureus genes with marine algae |
| url | http://psasir.upm.edu.my/id/eprint/13239/ http://psasir.upm.edu.my/id/eprint/13239/ http://psasir.upm.edu.my/id/eprint/13239/1/Application%20of%20RT.pdf |