Antiproliferative properties of clausine-B against cancer cell lines
Background: Clausine B, a carbazole alkaloid isolated from the stem bark of Clausena excavata, was investigated for its antiproliferative activities against five human cancer cell lines: HepG2 (hepatic cancer), MCF-7 (hormone-dependent breast cancer), MDA-MB-231 (non-hormone-dependent breast cance...
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Sains Malaysia
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13207/ http://psasir.upm.edu.my/id/eprint/13207/1/Antiproliferative%20properties%20of%20clausine.pdf |
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| author | Wan Mohamad Zain, Wan Nor I'zzah Rahmat, Asmah Othman, Fauziah Yap, Taufiq Yun Hin |
| author_facet | Wan Mohamad Zain, Wan Nor I'zzah Rahmat, Asmah Othman, Fauziah Yap, Taufiq Yun Hin |
| author_sort | Wan Mohamad Zain, Wan Nor I'zzah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background:
Clausine B, a carbazole alkaloid isolated from the stem bark of Clausena excavata, was investigated for its antiproliferative activities against five human cancer cell lines: HepG2 (hepatic cancer), MCF-7 (hormone-dependent breast cancer), MDA-MB-231 (non-hormone-dependent breast cancer), HeLa (cervical cancer), and CAOV3 (ovarian cancer).
Methods:
Chang liver (normal cells) was used as a control. The effect of clausine-B was measured using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.
Results:
Clausine-B was found to be active (IC 50<30 μg/mL) against four of the cancer cell lines tested. The IC 50 values for these four lines were: 21.50 μg/mL (MDA-MB-231), 22.90 g/ml (HeLa), 27.00 μg/mL (CAOV3) and 28.94 μg/mL (HepG2). Clausine-B inhibited the MCF-7 cancer cell line at 52.90 μg/mL, and no IC 50 value was obtained against Chang liver.
Conclusion:
It is possible that the phenolic group in clausine-B responsible for the antiproliferative activities found in this study. |
| first_indexed | 2025-11-15T07:52:57Z |
| format | Article |
| id | upm-13207 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:52:57Z |
| publishDate | 2009 |
| publisher | Penerbit Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-132072016-11-24T05:08:25Z http://psasir.upm.edu.my/id/eprint/13207/ Antiproliferative properties of clausine-B against cancer cell lines Wan Mohamad Zain, Wan Nor I'zzah Rahmat, Asmah Othman, Fauziah Yap, Taufiq Yun Hin Background: Clausine B, a carbazole alkaloid isolated from the stem bark of Clausena excavata, was investigated for its antiproliferative activities against five human cancer cell lines: HepG2 (hepatic cancer), MCF-7 (hormone-dependent breast cancer), MDA-MB-231 (non-hormone-dependent breast cancer), HeLa (cervical cancer), and CAOV3 (ovarian cancer). Methods: Chang liver (normal cells) was used as a control. The effect of clausine-B was measured using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Results: Clausine-B was found to be active (IC 50<30 μg/mL) against four of the cancer cell lines tested. The IC 50 values for these four lines were: 21.50 μg/mL (MDA-MB-231), 22.90 g/ml (HeLa), 27.00 μg/mL (CAOV3) and 28.94 μg/mL (HepG2). Clausine-B inhibited the MCF-7 cancer cell line at 52.90 μg/mL, and no IC 50 value was obtained against Chang liver. Conclusion: It is possible that the phenolic group in clausine-B responsible for the antiproliferative activities found in this study. Penerbit Universiti Sains Malaysia 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13207/1/Antiproliferative%20properties%20of%20clausine.pdf Wan Mohamad Zain, Wan Nor I'zzah and Rahmat, Asmah and Othman, Fauziah and Yap, Taufiq Yun Hin (2009) Antiproliferative properties of clausine-B against cancer cell lines. Malaysian Journal of Medical Sciences, 16 (3). pp. 31-36. ISSN 1394-195X; ESSN: 2180-4303 http://www.mjms.usm.my/default.asp?tag=14&kod_volume=29 |
| spellingShingle | Wan Mohamad Zain, Wan Nor I'zzah Rahmat, Asmah Othman, Fauziah Yap, Taufiq Yun Hin Antiproliferative properties of clausine-B against cancer cell lines |
| title | Antiproliferative properties of clausine-B against cancer cell lines |
| title_full | Antiproliferative properties of clausine-B against cancer cell lines |
| title_fullStr | Antiproliferative properties of clausine-B against cancer cell lines |
| title_full_unstemmed | Antiproliferative properties of clausine-B against cancer cell lines |
| title_short | Antiproliferative properties of clausine-B against cancer cell lines |
| title_sort | antiproliferative properties of clausine-b against cancer cell lines |
| url | http://psasir.upm.edu.my/id/eprint/13207/ http://psasir.upm.edu.my/id/eprint/13207/ http://psasir.upm.edu.my/id/eprint/13207/1/Antiproliferative%20properties%20of%20clausine.pdf |