Antiproliferative activity of pure lycopene compared to both extracted lycopene and juices from watermelon (Citrullus vulgaris) and papaya (Carica papaya) on human breast and liver cancer cell lines

Lycopene is one of the major carotenoids in the diet and is believed to have a number of health benefits including anticancer properties. This investigation was conducted with the aim of screening the potential anticancer properties of pure lycopene and of both extracted lycopene and juices from wat...

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Main Authors: Rahmat, Asmah, Rosli, Rozita, Wan Mohamad Zain, Wan Nor I'zzah, Endrini, Susi, Abdullah Sani, Huzaimah
Format: Article
Language:English
Published: Asian Network for Scientific Information 2002
Online Access:http://psasir.upm.edu.my/id/eprint/13204/
http://psasir.upm.edu.my/id/eprint/13204/1/Antiproliferative%20activity%20of%20pure%20lycopene%20compared%20to%20both%20extracted%20lycopene%20and%20juices%20from%20watermelon.pdf
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author Rahmat, Asmah
Rosli, Rozita
Wan Mohamad Zain, Wan Nor I'zzah
Endrini, Susi
Abdullah Sani, Huzaimah
author_facet Rahmat, Asmah
Rosli, Rozita
Wan Mohamad Zain, Wan Nor I'zzah
Endrini, Susi
Abdullah Sani, Huzaimah
author_sort Rahmat, Asmah
building UPM Institutional Repository
collection Online Access
description Lycopene is one of the major carotenoids in the diet and is believed to have a number of health benefits including anticancer properties. This investigation was conducted with the aim of screening the potential anticancer properties of pure lycopene and of both extracted lycopene and juices from watermelon and papaya. Two different types of human tumor cell lines, HepG2 (liver cancer cell line) and MDA-MB-231 (breast cancer cell line) were used for the evaluation of cytotoxicity effects. Chang liver cell line that is the transformed cell for the liver was used for comparison. The cells treated with pure lycopene, extracted lycopene and juices from both watermelon and papaya were maintained and incubated at 37°C in 5% CO2 for five days. The Microculture Tetrazolium salt (MTT) assay was carried out in this investigation, to determine the cell viability. Pure lycopene was found to cause 50% cell death (IC50) of HepG2 cells at a concentration of 22.8 μg ml-1, while papaya juice at a concentration of 20 mg ml-1. In the case of MDA-MB-231 cells, the IC50 of the watermelon was 11.3 mg ml-1. The other samples including extracted lycopene samples did not show any effect in the cell viability. The findings of this study showed that pure lycopene and papaya juice may have anticancer properties upon liver cancer cell line (HepG2), while watermelon juice had anticancer properties against breast cancer cell line (MDA-MB-231). The juices seemed to be more effective than the extracted lycopene samples in inhibiting cancer cell growth.
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spelling upm-132042016-11-24T05:15:06Z http://psasir.upm.edu.my/id/eprint/13204/ Antiproliferative activity of pure lycopene compared to both extracted lycopene and juices from watermelon (Citrullus vulgaris) and papaya (Carica papaya) on human breast and liver cancer cell lines Rahmat, Asmah Rosli, Rozita Wan Mohamad Zain, Wan Nor I'zzah Endrini, Susi Abdullah Sani, Huzaimah Lycopene is one of the major carotenoids in the diet and is believed to have a number of health benefits including anticancer properties. This investigation was conducted with the aim of screening the potential anticancer properties of pure lycopene and of both extracted lycopene and juices from watermelon and papaya. Two different types of human tumor cell lines, HepG2 (liver cancer cell line) and MDA-MB-231 (breast cancer cell line) were used for the evaluation of cytotoxicity effects. Chang liver cell line that is the transformed cell for the liver was used for comparison. The cells treated with pure lycopene, extracted lycopene and juices from both watermelon and papaya were maintained and incubated at 37°C in 5% CO2 for five days. The Microculture Tetrazolium salt (MTT) assay was carried out in this investigation, to determine the cell viability. Pure lycopene was found to cause 50% cell death (IC50) of HepG2 cells at a concentration of 22.8 μg ml-1, while papaya juice at a concentration of 20 mg ml-1. In the case of MDA-MB-231 cells, the IC50 of the watermelon was 11.3 mg ml-1. The other samples including extracted lycopene samples did not show any effect in the cell viability. The findings of this study showed that pure lycopene and papaya juice may have anticancer properties upon liver cancer cell line (HepG2), while watermelon juice had anticancer properties against breast cancer cell line (MDA-MB-231). The juices seemed to be more effective than the extracted lycopene samples in inhibiting cancer cell growth. Asian Network for Scientific Information 2002 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13204/1/Antiproliferative%20activity%20of%20pure%20lycopene%20compared%20to%20both%20extracted%20lycopene%20and%20juices%20from%20watermelon.pdf Rahmat, Asmah and Rosli, Rozita and Wan Mohamad Zain, Wan Nor I'zzah and Endrini, Susi and Abdullah Sani, Huzaimah (2002) Antiproliferative activity of pure lycopene compared to both extracted lycopene and juices from watermelon (Citrullus vulgaris) and papaya (Carica papaya) on human breast and liver cancer cell lines. Journal of Medical Sciences, 2 (2). pp. 55-58. ISSN 1682-4474; ESSN: 1812-5727 http://scialert.net/abstract/?doi=jms.2002.55.58 10.3923/jms.2002.55.58
spellingShingle Rahmat, Asmah
Rosli, Rozita
Wan Mohamad Zain, Wan Nor I'zzah
Endrini, Susi
Abdullah Sani, Huzaimah
Antiproliferative activity of pure lycopene compared to both extracted lycopene and juices from watermelon (Citrullus vulgaris) and papaya (Carica papaya) on human breast and liver cancer cell lines
title Antiproliferative activity of pure lycopene compared to both extracted lycopene and juices from watermelon (Citrullus vulgaris) and papaya (Carica papaya) on human breast and liver cancer cell lines
title_full Antiproliferative activity of pure lycopene compared to both extracted lycopene and juices from watermelon (Citrullus vulgaris) and papaya (Carica papaya) on human breast and liver cancer cell lines
title_fullStr Antiproliferative activity of pure lycopene compared to both extracted lycopene and juices from watermelon (Citrullus vulgaris) and papaya (Carica papaya) on human breast and liver cancer cell lines
title_full_unstemmed Antiproliferative activity of pure lycopene compared to both extracted lycopene and juices from watermelon (Citrullus vulgaris) and papaya (Carica papaya) on human breast and liver cancer cell lines
title_short Antiproliferative activity of pure lycopene compared to both extracted lycopene and juices from watermelon (Citrullus vulgaris) and papaya (Carica papaya) on human breast and liver cancer cell lines
title_sort antiproliferative activity of pure lycopene compared to both extracted lycopene and juices from watermelon (citrullus vulgaris) and papaya (carica papaya) on human breast and liver cancer cell lines
url http://psasir.upm.edu.my/id/eprint/13204/
http://psasir.upm.edu.my/id/eprint/13204/
http://psasir.upm.edu.my/id/eprint/13204/
http://psasir.upm.edu.my/id/eprint/13204/1/Antiproliferative%20activity%20of%20pure%20lycopene%20compared%20to%20both%20extracted%20lycopene%20and%20juices%20from%20watermelon.pdf