Antimicrobial activity of Cinnamomum impressicostatum and C. pubescens and bioassay-guided isolation of bioactive methyl-cinnamate
The air dried barks of Cinnamomum impressicostatum and C. pubescens were separately and sequentially extracted with hexane, chloroform and methanol and assayed against five bacteria and four fungi by disc diffusion method. The hexane and chloroform extracts of the plants strongly suppressed the grow...
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| Format: | Article |
| Language: | English |
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Asian Network for Scientific Information
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13159/ http://psasir.upm.edu.my/id/eprint/13159/1/Antimicrobial%20activity%20of%20Cinnamomum%20impressicostatum%20and%20C.pdf |
| _version_ | 1848842035760463872 |
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| author | Ali, N. A. M. Rahmani, Mawardi Shaari, Khozirah Ahmed Ali, Asem Mohamed Ee, Gwendoline Cheng Lian |
| author_facet | Ali, N. A. M. Rahmani, Mawardi Shaari, Khozirah Ahmed Ali, Asem Mohamed Ee, Gwendoline Cheng Lian |
| author_sort | Ali, N. A. M. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The air dried barks of Cinnamomum impressicostatum and C. pubescens were separately and sequentially extracted with hexane, chloroform and methanol and assayed against five bacteria and four fungi by disc diffusion method. The hexane and chloroform extracts of the plants strongly suppressed the growth of the four fungi but range from moderate to weak towards bacteria. The methanol extracts only displayed weak activity on some of these organisms. Based on these results, further work was carried on bioassay-guided isolation of active extracts of C. impressicostatum and C. pubescens and on both occasions the active component was identified as (E)-methyl cinnamate. Other compounds were also isolated and identified as safrole, (E)-piperonylprop-2-enal, β-sitosterol, (E)-piperonylprop-2-enol and cinnamic acid. The structures of the compounds were established by spectroscopic method and comparison with literatures. (E)-methyl cinnamate exhibited strong growth inhibition towards four of the fungi Saccharomyces cerevisiae, Candida lipolytica, C. albicans, Microsporum canis with inhibition zone ranging from 24.5 to 19.8 mm. The compound is also capable of suppressing the growth of bacteria Bacillus cereus. It is proposed that the excellent antifungal activities of the hexane and chloroform extracts of C. impressicostatum and C. pubescens correlate with the presence of (E)-methyl cinnamate as the major component. |
| first_indexed | 2025-11-15T07:52:44Z |
| format | Article |
| id | upm-13159 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:52:44Z |
| publishDate | 2010 |
| publisher | Asian Network for Scientific Information |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-131592015-10-26T06:27:24Z http://psasir.upm.edu.my/id/eprint/13159/ Antimicrobial activity of Cinnamomum impressicostatum and C. pubescens and bioassay-guided isolation of bioactive methyl-cinnamate Ali, N. A. M. Rahmani, Mawardi Shaari, Khozirah Ahmed Ali, Asem Mohamed Ee, Gwendoline Cheng Lian The air dried barks of Cinnamomum impressicostatum and C. pubescens were separately and sequentially extracted with hexane, chloroform and methanol and assayed against five bacteria and four fungi by disc diffusion method. The hexane and chloroform extracts of the plants strongly suppressed the growth of the four fungi but range from moderate to weak towards bacteria. The methanol extracts only displayed weak activity on some of these organisms. Based on these results, further work was carried on bioassay-guided isolation of active extracts of C. impressicostatum and C. pubescens and on both occasions the active component was identified as (E)-methyl cinnamate. Other compounds were also isolated and identified as safrole, (E)-piperonylprop-2-enal, β-sitosterol, (E)-piperonylprop-2-enol and cinnamic acid. The structures of the compounds were established by spectroscopic method and comparison with literatures. (E)-methyl cinnamate exhibited strong growth inhibition towards four of the fungi Saccharomyces cerevisiae, Candida lipolytica, C. albicans, Microsporum canis with inhibition zone ranging from 24.5 to 19.8 mm. The compound is also capable of suppressing the growth of bacteria Bacillus cereus. It is proposed that the excellent antifungal activities of the hexane and chloroform extracts of C. impressicostatum and C. pubescens correlate with the presence of (E)-methyl cinnamate as the major component. Asian Network for Scientific Information 2010-02-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13159/1/Antimicrobial%20activity%20of%20Cinnamomum%20impressicostatum%20and%20C.pdf Ali, N. A. M. and Rahmani, Mawardi and Shaari, Khozirah and Ahmed Ali, Asem Mohamed and Ee, Gwendoline Cheng Lian (2010) Antimicrobial activity of Cinnamomum impressicostatum and C. pubescens and bioassay-guided isolation of bioactive methyl-cinnamate. Journal of Biological Scince, 10 (2). pp. 101-106. ISSN 1727-3048; ESSN: 1812-5719 http://scialert.net/abstract/?doi=jbs.2010.101.106 10.3923/jbs.2010.101.106 |
| spellingShingle | Ali, N. A. M. Rahmani, Mawardi Shaari, Khozirah Ahmed Ali, Asem Mohamed Ee, Gwendoline Cheng Lian Antimicrobial activity of Cinnamomum impressicostatum and C. pubescens and bioassay-guided isolation of bioactive methyl-cinnamate |
| title | Antimicrobial activity of Cinnamomum impressicostatum and C. pubescens and bioassay-guided isolation of bioactive methyl-cinnamate |
| title_full | Antimicrobial activity of Cinnamomum impressicostatum and C. pubescens and bioassay-guided isolation of bioactive methyl-cinnamate |
| title_fullStr | Antimicrobial activity of Cinnamomum impressicostatum and C. pubescens and bioassay-guided isolation of bioactive methyl-cinnamate |
| title_full_unstemmed | Antimicrobial activity of Cinnamomum impressicostatum and C. pubescens and bioassay-guided isolation of bioactive methyl-cinnamate |
| title_short | Antimicrobial activity of Cinnamomum impressicostatum and C. pubescens and bioassay-guided isolation of bioactive methyl-cinnamate |
| title_sort | antimicrobial activity of cinnamomum impressicostatum and c. pubescens and bioassay-guided isolation of bioactive methyl-cinnamate |
| url | http://psasir.upm.edu.my/id/eprint/13159/ http://psasir.upm.edu.my/id/eprint/13159/ http://psasir.upm.edu.my/id/eprint/13159/ http://psasir.upm.edu.my/id/eprint/13159/1/Antimicrobial%20activity%20of%20Cinnamomum%20impressicostatum%20and%20C.pdf |