Anticancer activity of natural compound (zerumbone) extracted from Zingiber zerumbet in human HeLa cervical cancer cells
A natural compound, zerumbone was extracted, isolated and purified from the rhizomes of edible plant Zingiber zerumbet using methanol extraction and Column Chromatography (CC) method. The isolated and purified zerumbone crystals were subjected to High Performance Liquid Chromatography (HPLC), Liquid...
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| Format: | Article |
| Language: | English |
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Asian Network for Scientific Information
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13138/ http://psasir.upm.edu.my/id/eprint/13138/1/Anticancer%20activity%20of%20natural%20compound.pdf |
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| author | Abdul, Ahmad Bustamam Al-Zubairi, Adel Sharaf Tailan, Nirmala Devi Abdel Wahab, Siddig Ibrahim Mohd Zain, Zetty Nadia Ruslay, Sharin Murali Mohan, Syam Mohan |
| author_facet | Abdul, Ahmad Bustamam Al-Zubairi, Adel Sharaf Tailan, Nirmala Devi Abdel Wahab, Siddig Ibrahim Mohd Zain, Zetty Nadia Ruslay, Sharin Murali Mohan, Syam Mohan |
| author_sort | Abdul, Ahmad Bustamam |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A natural compound, zerumbone was extracted, isolated and purified from the rhizomes of edible plant Zingiber zerumbet using methanol extraction and Column Chromatography (CC) method. The isolated and purified zerumbone crystals were subjected to High Performance Liquid Chromatography (HPLC), Liquid Chromatography Mass Spectrometry (LCMS) and 13C NMR and 1H NMR analysis to confirm the purity, molecular weight and molecular structure. The study investigated the purified zerumbone crystals for its anti-cancer properties on human cervical cancer cell line (HeLa). Cisplatin, was used as a positive control in this study. The cytotoxicity of zerumbone and cisplatin were investigated using the MTT assay and caspases-3 was estimated with colorimetric assay in zerumbone treated HeLa cells. Morphological analysis showed that there were changes observed on HeLa cancer cells after treatment with zerumbone and cisplatin. The MTT assay results demonstrated that the IC50 value ( ± SEM) of zerumbone was determined to be 11.3 μM (2.5 μg mL-1) whilst the IC50 value of cisplatin was at 7.5 μM (1.6 μg mL-1). Prominent growth retardation was identified to the HeLa cancer cells, after treatment with both compounds, while caspase-3 was observed to be significantly increased in zerumbone treated cells as compared to untreated control cells. This study showed promising avenues towards zerumbone to be developed as a new chemo-natural drug for treatment of cervical cancer. |
| first_indexed | 2025-11-15T07:52:38Z |
| format | Article |
| id | upm-13138 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:52:38Z |
| publishDate | 2008 |
| publisher | Asian Network for Scientific Information |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-131382015-12-02T04:41:11Z http://psasir.upm.edu.my/id/eprint/13138/ Anticancer activity of natural compound (zerumbone) extracted from Zingiber zerumbet in human HeLa cervical cancer cells Abdul, Ahmad Bustamam Al-Zubairi, Adel Sharaf Tailan, Nirmala Devi Abdel Wahab, Siddig Ibrahim Mohd Zain, Zetty Nadia Ruslay, Sharin Murali Mohan, Syam Mohan A natural compound, zerumbone was extracted, isolated and purified from the rhizomes of edible plant Zingiber zerumbet using methanol extraction and Column Chromatography (CC) method. The isolated and purified zerumbone crystals were subjected to High Performance Liquid Chromatography (HPLC), Liquid Chromatography Mass Spectrometry (LCMS) and 13C NMR and 1H NMR analysis to confirm the purity, molecular weight and molecular structure. The study investigated the purified zerumbone crystals for its anti-cancer properties on human cervical cancer cell line (HeLa). Cisplatin, was used as a positive control in this study. The cytotoxicity of zerumbone and cisplatin were investigated using the MTT assay and caspases-3 was estimated with colorimetric assay in zerumbone treated HeLa cells. Morphological analysis showed that there were changes observed on HeLa cancer cells after treatment with zerumbone and cisplatin. The MTT assay results demonstrated that the IC50 value ( ± SEM) of zerumbone was determined to be 11.3 μM (2.5 μg mL-1) whilst the IC50 value of cisplatin was at 7.5 μM (1.6 μg mL-1). Prominent growth retardation was identified to the HeLa cancer cells, after treatment with both compounds, while caspase-3 was observed to be significantly increased in zerumbone treated cells as compared to untreated control cells. This study showed promising avenues towards zerumbone to be developed as a new chemo-natural drug for treatment of cervical cancer. Asian Network for Scientific Information 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13138/1/Anticancer%20activity%20of%20natural%20compound.pdf Abdul, Ahmad Bustamam and Al-Zubairi, Adel Sharaf and Tailan, Nirmala Devi and Abdel Wahab, Siddig Ibrahim and Mohd Zain, Zetty Nadia and Ruslay, Sharin and Murali Mohan, Syam Mohan (2008) Anticancer activity of natural compound (zerumbone) extracted from Zingiber zerumbet in human HeLa cervical cancer cells. International Journal of Pharmacology, 4 (3). pp. 160-168. ISSN 1811-7775; ESSN: 1812-5700 http://scialert.net/abstract/?doi=ijp.2008.160.168 10.3923/ijp.2008.160.168 |
| spellingShingle | Abdul, Ahmad Bustamam Al-Zubairi, Adel Sharaf Tailan, Nirmala Devi Abdel Wahab, Siddig Ibrahim Mohd Zain, Zetty Nadia Ruslay, Sharin Murali Mohan, Syam Mohan Anticancer activity of natural compound (zerumbone) extracted from Zingiber zerumbet in human HeLa cervical cancer cells |
| title | Anticancer activity of natural compound (zerumbone) extracted from Zingiber zerumbet in human HeLa cervical cancer cells |
| title_full | Anticancer activity of natural compound (zerumbone) extracted from Zingiber zerumbet in human HeLa cervical cancer cells |
| title_fullStr | Anticancer activity of natural compound (zerumbone) extracted from Zingiber zerumbet in human HeLa cervical cancer cells |
| title_full_unstemmed | Anticancer activity of natural compound (zerumbone) extracted from Zingiber zerumbet in human HeLa cervical cancer cells |
| title_short | Anticancer activity of natural compound (zerumbone) extracted from Zingiber zerumbet in human HeLa cervical cancer cells |
| title_sort | anticancer activity of natural compound (zerumbone) extracted from zingiber zerumbet in human hela cervical cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/13138/ http://psasir.upm.edu.my/id/eprint/13138/ http://psasir.upm.edu.my/id/eprint/13138/ http://psasir.upm.edu.my/id/eprint/13138/1/Anticancer%20activity%20of%20natural%20compound.pdf |