Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia

Vibrio parahaemolyticus is one of the most widely recognized pathogenic Vibrio species due to numerous outbreaks and its’ wide occurrence in marine environment. In this study, 32 isolates of V. parahaemolyticus isolated from cockles were tested for sensitivity to 16 antibiotics and the presence of p...

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Main Authors: Yusop, Zulkifli, Mohammed Alitheen, Noorjahan Banu, Abdul Rahim, Raha, Yeap, Swee Keong, Marlina, Radu, Son, Nishibuchi, Mitsuaki
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2009
Online Access:http://psasir.upm.edu.my/id/eprint/13136/
http://psasir.upm.edu.my/id/eprint/13136/1/13136.pdf
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author Yusop, Zulkifli
Mohammed Alitheen, Noorjahan Banu
Abdul Rahim, Raha
Yeap, Swee Keong
Marlina
Radu, Son
Nishibuchi, Mitsuaki
author_facet Yusop, Zulkifli
Mohammed Alitheen, Noorjahan Banu
Abdul Rahim, Raha
Yeap, Swee Keong
Marlina
Radu, Son
Nishibuchi, Mitsuaki
author_sort Yusop, Zulkifli
building UPM Institutional Repository
collection Online Access
description Vibrio parahaemolyticus is one of the most widely recognized pathogenic Vibrio species due to numerous outbreaks and its’ wide occurrence in marine environment. In this study, 32 isolates of V. parahaemolyticus isolated from cockles were tested for sensitivity to 16 antibiotics and the presence of plasmids. All the isolates were multi-resistance, defined as resistant to at least three different antibiotics with multiple antibiotic resistance indexes ranging from 0.31 to 0.69, indicating the isolates originate from high risk sources of contamination where antibiotics are often used. In the plasmid profiling test, only 15 isolates (47%) harbored plasmid DNA, which ranged in size from 2.7 to 56.2 kb, separating the isolates into 14 plasmid profiles. Hence, food contaminated with antibiotic resistant V. parahaemolyticus could be a major threat to public health due to the distinct possibility that they can be a significant reservoir of genes encoding antibiotic resistance determinants that can be transferred intra or interspecies. As in many developing countries, raw food hygiene and antimicrobial resistance epidemiology is still in the infancy stage in the locality of the study and thus our data provide a current baseline profile of antimicrobial resistance and plasmid of V. parahaemolyticus from cockles in Padang, Indonesia.
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spelling upm-131362020-05-15T04:02:05Z http://psasir.upm.edu.my/id/eprint/13136/ Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia Yusop, Zulkifli Mohammed Alitheen, Noorjahan Banu Abdul Rahim, Raha Yeap, Swee Keong Marlina Radu, Son Nishibuchi, Mitsuaki Vibrio parahaemolyticus is one of the most widely recognized pathogenic Vibrio species due to numerous outbreaks and its’ wide occurrence in marine environment. In this study, 32 isolates of V. parahaemolyticus isolated from cockles were tested for sensitivity to 16 antibiotics and the presence of plasmids. All the isolates were multi-resistance, defined as resistant to at least three different antibiotics with multiple antibiotic resistance indexes ranging from 0.31 to 0.69, indicating the isolates originate from high risk sources of contamination where antibiotics are often used. In the plasmid profiling test, only 15 isolates (47%) harbored plasmid DNA, which ranged in size from 2.7 to 56.2 kb, separating the isolates into 14 plasmid profiles. Hence, food contaminated with antibiotic resistant V. parahaemolyticus could be a major threat to public health due to the distinct possibility that they can be a significant reservoir of genes encoding antibiotic resistance determinants that can be transferred intra or interspecies. As in many developing countries, raw food hygiene and antimicrobial resistance epidemiology is still in the infancy stage in the locality of the study and thus our data provide a current baseline profile of antimicrobial resistance and plasmid of V. parahaemolyticus from cockles in Padang, Indonesia. Faculty of Food Science and Technology, Universiti Putra Malaysia 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13136/1/13136.pdf Yusop, Zulkifli and Mohammed Alitheen, Noorjahan Banu and Abdul Rahim, Raha and Yeap, Swee Keong and Marlina and Radu, Son and Nishibuchi, Mitsuaki (2009) Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia. International Food Research Journal, 16 (1). pp. 53-58. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/16%20%281%29%202009/07%20IFRJ-2008-124%20Zulkifli%20Malaysia%20FINAL.pdf
spellingShingle Yusop, Zulkifli
Mohammed Alitheen, Noorjahan Banu
Abdul Rahim, Raha
Yeap, Swee Keong
Marlina
Radu, Son
Nishibuchi, Mitsuaki
Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
title Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
title_full Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
title_fullStr Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
title_full_unstemmed Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
title_short Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
title_sort antibiotic resistance and plasmid profiling of vibrio parahaemolyticus isolated from cockles in padang, indonesia
url http://psasir.upm.edu.my/id/eprint/13136/
http://psasir.upm.edu.my/id/eprint/13136/
http://psasir.upm.edu.my/id/eprint/13136/1/13136.pdf