Antagonistic potential of selected fungal and bacterial biocontrol agents against Colletotrichum truncatum of soybean seeds
Ten fungal and bacterial biocontrol agents (BCAs) were evaluated in vitro for their antagonistic potential against Colletotrichum truncatum isolated from soybean seeds. Two fungal BCAs namely Trichoderma virens isolate UPM23 and Trichoderma harzianum isolate UPM40 and a bacterial BCA namely Pseudomo...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13116/ http://psasir.upm.edu.my/id/eprint/13116/1/Antagonistic%20Potential%20of%20Selected%20Fungal%20and%20Bacterial%20Biocontrol%20Agents%20against%20Colletotrichum%20truncatum%20of%20Soybean%20Seeds.pdf |
| _version_ | 1848842023999635456 |
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| author | Begum, Most Mahbuba Meon, Sariah Mior Ahmad, Zainal Abidin Puteh, Adam A. Rahman, Mahfar |
| author_facet | Begum, Most Mahbuba Meon, Sariah Mior Ahmad, Zainal Abidin Puteh, Adam A. Rahman, Mahfar |
| author_sort | Begum, Most Mahbuba |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Ten fungal and bacterial biocontrol agents (BCAs) were evaluated in vitro for their antagonistic potential against Colletotrichum truncatum isolated from soybean seeds. Two fungal BCAs namely Trichoderma virens isolate UPM23 and Trichoderma harzianum isolate UPM40 and a bacterial BCA namely Pseudomonas aeruginosa isolate UPM13B8 strongly inhibited the growth of C. truncatum based on high PIRG values in dual culture and culture filtrate tests. Studies on the mechanism of action using mycoparasitism technique and antibiosis observed under light microscope revealed that T. virens and T. harzianum inhibited the growth of C. truncatum by coiling and penetration into the hyphae. Consequently, the hyphae of C. truncatum became malformed and swollen. Pseudomonas aeruginosa also caused mycelial malformation; the mycelia turned vacuoled and swollen in or at tips of hyphae. Pseudomonas aeruginosa gave the highest PIRG value in the culture filtrate test, suggesting that antibiosis could be the main mechanism of antagonism. No inhibitory effect was observed on soybean seeds and seedlings when the seeds were artificially inoculated with T. virens, T. harzianum and P. aeruginosa. On the contrary, T. virens and T. harzianum were found to enhance seed germination and seedling establishment, while P. aeruginosa enhanced fresh and dry weights of seedlings. |
| first_indexed | 2025-11-15T07:52:33Z |
| format | Article |
| id | upm-13116 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:52:33Z |
| publishDate | 2008 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-131162015-09-18T02:27:25Z http://psasir.upm.edu.my/id/eprint/13116/ Antagonistic potential of selected fungal and bacterial biocontrol agents against Colletotrichum truncatum of soybean seeds Begum, Most Mahbuba Meon, Sariah Mior Ahmad, Zainal Abidin Puteh, Adam A. Rahman, Mahfar Ten fungal and bacterial biocontrol agents (BCAs) were evaluated in vitro for their antagonistic potential against Colletotrichum truncatum isolated from soybean seeds. Two fungal BCAs namely Trichoderma virens isolate UPM23 and Trichoderma harzianum isolate UPM40 and a bacterial BCA namely Pseudomonas aeruginosa isolate UPM13B8 strongly inhibited the growth of C. truncatum based on high PIRG values in dual culture and culture filtrate tests. Studies on the mechanism of action using mycoparasitism technique and antibiosis observed under light microscope revealed that T. virens and T. harzianum inhibited the growth of C. truncatum by coiling and penetration into the hyphae. Consequently, the hyphae of C. truncatum became malformed and swollen. Pseudomonas aeruginosa also caused mycelial malformation; the mycelia turned vacuoled and swollen in or at tips of hyphae. Pseudomonas aeruginosa gave the highest PIRG value in the culture filtrate test, suggesting that antibiosis could be the main mechanism of antagonism. No inhibitory effect was observed on soybean seeds and seedlings when the seeds were artificially inoculated with T. virens, T. harzianum and P. aeruginosa. On the contrary, T. virens and T. harzianum were found to enhance seed germination and seedling establishment, while P. aeruginosa enhanced fresh and dry weights of seedlings. Universiti Putra Malaysia Press 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13116/1/Antagonistic%20Potential%20of%20Selected%20Fungal%20and%20Bacterial%20Biocontrol%20Agents%20against%20Colletotrichum%20truncatum%20of%20Soybean%20Seeds.pdf Begum, Most Mahbuba and Meon, Sariah and Mior Ahmad, Zainal Abidin and Puteh, Adam and A. Rahman, Mahfar (2008) Antagonistic potential of selected fungal and bacterial biocontrol agents against Colletotrichum truncatum of soybean seeds. Pertanika Journal of Tropical Agricultural Science, 31 (1). pp. 45-53. ISSN 1511-3701 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2031%20(1)%20Feb.%202008/09%20PAGE%2045-53.pdf |
| spellingShingle | Begum, Most Mahbuba Meon, Sariah Mior Ahmad, Zainal Abidin Puteh, Adam A. Rahman, Mahfar Antagonistic potential of selected fungal and bacterial biocontrol agents against Colletotrichum truncatum of soybean seeds |
| title | Antagonistic potential of selected fungal and bacterial biocontrol agents against Colletotrichum truncatum of soybean seeds |
| title_full | Antagonistic potential of selected fungal and bacterial biocontrol agents against Colletotrichum truncatum of soybean seeds |
| title_fullStr | Antagonistic potential of selected fungal and bacterial biocontrol agents against Colletotrichum truncatum of soybean seeds |
| title_full_unstemmed | Antagonistic potential of selected fungal and bacterial biocontrol agents against Colletotrichum truncatum of soybean seeds |
| title_short | Antagonistic potential of selected fungal and bacterial biocontrol agents against Colletotrichum truncatum of soybean seeds |
| title_sort | antagonistic potential of selected fungal and bacterial biocontrol agents against colletotrichum truncatum of soybean seeds |
| url | http://psasir.upm.edu.my/id/eprint/13116/ http://psasir.upm.edu.my/id/eprint/13116/ http://psasir.upm.edu.my/id/eprint/13116/1/Antagonistic%20Potential%20of%20Selected%20Fungal%20and%20Bacterial%20Biocontrol%20Agents%20against%20Colletotrichum%20truncatum%20of%20Soybean%20Seeds.pdf |