An inhibitive determination method for heavy metals using bromelain, a cysteine protease

An inhibitive determination method for heavy metals using bromelain, a cysteine protease

A heavy-metal assay has been developed using bromelain, a protease. The enzyme is assayed using casein as a substrate with Coomassie dye to track completion of hydrolysis of casein. In the absence of inhibitors, casein is hydrolysed to completion, and the solution is brown. In the presence of metal...

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Bibliographic Details
Main Authors: Abd. Shukor, Mohd. Yunus, Masdor, Noor Azlina, Baharom, Nor Azlan, Jamal, Jamia Azdina, Abdullah, Mohd Puad, Shamaan, Nor Aripin, Syed, Mohd Arif
Format: Article
Language:English
Published: Humana Press 2008
Online Access:http://psasir.upm.edu.my/id/eprint/13043/
http://psasir.upm.edu.my/id/eprint/13043/1/An%20inhibitive%20determination%20method%20for%20heavy%20metals%20using%20bromelain.pdf

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