An evaluation of beta-hydroxybutyrate in milk and blood for prediction of subclinical ketosis in dairy cows.

The first objective of this study was to investigate the relationship between concentrations of beta-hydroxybutyrate (BHBA) in milk and blood to assess the reliability of the BHBA concentrations in milk measured by a semi quantitative keto-test paper to detect subclinical ketosis (SCK) in 50 fresh h...

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Main Authors: Samiei, A., Juan, Boo Liang, Ghorbani, G. R., Hirooka, H., Yaakub, Halimatun, Tabatabaei, Meisam
Format: Article
Language:English
Published: http://www.uwm.edu.pl/pjvsci/main.html 2010
Online Access:http://psasir.upm.edu.my/id/eprint/13030/
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author Samiei, A.
Juan, Boo Liang
Ghorbani, G. R.
Hirooka, H.
Yaakub, Halimatun
Tabatabaei, Meisam
author_facet Samiei, A.
Juan, Boo Liang
Ghorbani, G. R.
Hirooka, H.
Yaakub, Halimatun
Tabatabaei, Meisam
author_sort Samiei, A.
building UPM Institutional Repository
collection Online Access
description The first objective of this study was to investigate the relationship between concentrations of beta-hydroxybutyrate (BHBA) in milk and blood to assess the reliability of the BHBA concentrations in milk measured by a semi quantitative keto-test paper to detect subclinical ketosis (SCK) in 50 fresh high-producing Iranian Holstein cows in Golestan Province, Iran. The second objective was the effects of SCK on milk yield and components. Concentrations of nonesterified fatty acids (NEFA) and BHBA were analyzed quantitatively in blood plasma and commercial keto-test paper was used for semi quantitative determination of BHBA concentration in milk. Milk yield was measured until 60 d after calving but milk compositions were measured until 30 d after calving. The mean plasma BHBA, milk BHBA, plasma NEFA, milk yield, milk fat percentage and milk fat: protein ratio were 1,234 micromol/L, 145 micromol/L, 0.482 mEq/L, 29.5 kg, 3.9% and 1.4, respectively. Fifty eight percent of the cows had SCK during the first month of lactation. High correlation coefficients were observed between blood BHBA and blood NEFA, and between blood and milk BHBA. The milk yield of cattle with SCK decreased (P < 0.01) but the fat percentage and milk fat: protein ratio increased (P < 0.01). The commercial keto-test paper used had a low false positive result at a cut-off point of 200 fmol of BHBA/L of milk. The results showed that the best time to assess SCK using the commercial keto-test paper was d 10, 14 and 17 after calving.
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spelling upm-130302014-04-23T08:29:43Z http://psasir.upm.edu.my/id/eprint/13030/ An evaluation of beta-hydroxybutyrate in milk and blood for prediction of subclinical ketosis in dairy cows. Samiei, A. Juan, Boo Liang Ghorbani, G. R. Hirooka, H. Yaakub, Halimatun Tabatabaei, Meisam The first objective of this study was to investigate the relationship between concentrations of beta-hydroxybutyrate (BHBA) in milk and blood to assess the reliability of the BHBA concentrations in milk measured by a semi quantitative keto-test paper to detect subclinical ketosis (SCK) in 50 fresh high-producing Iranian Holstein cows in Golestan Province, Iran. The second objective was the effects of SCK on milk yield and components. Concentrations of nonesterified fatty acids (NEFA) and BHBA were analyzed quantitatively in blood plasma and commercial keto-test paper was used for semi quantitative determination of BHBA concentration in milk. Milk yield was measured until 60 d after calving but milk compositions were measured until 30 d after calving. The mean plasma BHBA, milk BHBA, plasma NEFA, milk yield, milk fat percentage and milk fat: protein ratio were 1,234 micromol/L, 145 micromol/L, 0.482 mEq/L, 29.5 kg, 3.9% and 1.4, respectively. Fifty eight percent of the cows had SCK during the first month of lactation. High correlation coefficients were observed between blood BHBA and blood NEFA, and between blood and milk BHBA. The milk yield of cattle with SCK decreased (P < 0.01) but the fat percentage and milk fat: protein ratio increased (P < 0.01). The commercial keto-test paper used had a low false positive result at a cut-off point of 200 fmol of BHBA/L of milk. The results showed that the best time to assess SCK using the commercial keto-test paper was d 10, 14 and 17 after calving. http://www.uwm.edu.pl/pjvsci/main.html 2010 Article PeerReviewed Samiei, A. and Juan, Boo Liang and Ghorbani, G. R. and Hirooka, H. and Yaakub, Halimatun and Tabatabaei, Meisam (2010) An evaluation of beta-hydroxybutyrate in milk and blood for prediction of subclinical ketosis in dairy cows. Polish Journal of Veterinary Sciences, 13 (2). pp. 349-356. ISSN 1505-1773 English
spellingShingle Samiei, A.
Juan, Boo Liang
Ghorbani, G. R.
Hirooka, H.
Yaakub, Halimatun
Tabatabaei, Meisam
An evaluation of beta-hydroxybutyrate in milk and blood for prediction of subclinical ketosis in dairy cows.
title An evaluation of beta-hydroxybutyrate in milk and blood for prediction of subclinical ketosis in dairy cows.
title_full An evaluation of beta-hydroxybutyrate in milk and blood for prediction of subclinical ketosis in dairy cows.
title_fullStr An evaluation of beta-hydroxybutyrate in milk and blood for prediction of subclinical ketosis in dairy cows.
title_full_unstemmed An evaluation of beta-hydroxybutyrate in milk and blood for prediction of subclinical ketosis in dairy cows.
title_short An evaluation of beta-hydroxybutyrate in milk and blood for prediction of subclinical ketosis in dairy cows.
title_sort evaluation of beta-hydroxybutyrate in milk and blood for prediction of subclinical ketosis in dairy cows.
url http://psasir.upm.edu.my/id/eprint/13030/