Antibacterial and sporicidal activity of Eugenia polyantha Wight against Bacillus cereus and Bacillus subtilis
The spore-forming bacteria, Bacillus spp., have often been associated with the contamination of rice and other starchy products. In this study, the extract of Indonesian bay leaf (Eugenia polyantha) was assessed for its antibacterial and sporicidal activities against vegetative cells and spores of B...
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| Format: | Article |
| Language: | English |
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Excellent Publishers
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12929/ http://psasir.upm.edu.my/id/eprint/12929/1/Antibacterial%20and%20sporicidal%20activity%20of%20Eugenia%20polyantha%20Wight%20against%20Bacillus%20cereus%20and%20Bacillus%20subtilis.pdf |
| _version_ | 1848841971078004736 |
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| author | Lau, Kah Yan Mohd Zainin, Nurul Syazwani Abas, Faridah Rukayadi, Yaya |
| author_facet | Lau, Kah Yan Mohd Zainin, Nurul Syazwani Abas, Faridah Rukayadi, Yaya |
| author_sort | Lau, Kah Yan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The spore-forming bacteria, Bacillus spp., have often been associated with the contamination of rice and other starchy products. In this study, the extract of Indonesian bay leaf (Eugenia polyantha) was assessed for its antibacterial and sporicidal activities against vegetative cells and spores of Bacillus cereus ATCC33019 as well as Bacillus subtilis ATCC6633. The Minimum Inhibitory Concentration of E. polyantha extract against vegetative cells of B. cereus and B. subtilis were 0.31 and 0.63 mg/mL, respectively, while both their Minimum Bactericidal Concentration were 2.50 mg/mL. Time kill curves demonstrated that the bactericidal endpoint for B. cereus and B. subtilis was reached at concentration of 2.50 and 5.00 mg/mL, respectively (8× MIC) after 4 h of incubation. E. polyantha extract inactivated more than 3-log (99.99%) of B. cereus and B. subtilis spores at a concentration of 10.00 mg/mL (1.0%) after 1 h of incubation and the spores was completely killed at 25.00 mg/mL (2.5%). The sporicidal activity of E. polyantha extract was not affected by different temperatures treatment and alteration of pH. In conclusion, the potential anti-Bacillus activities of E. polyantha extract might support its use as a natural food preservative. |
| first_indexed | 2025-11-15T07:51:42Z |
| format | Article |
| id | upm-12929 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:51:42Z |
| publishDate | 2014 |
| publisher | Excellent Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-129292018-06-11T08:35:16Z http://psasir.upm.edu.my/id/eprint/12929/ Antibacterial and sporicidal activity of Eugenia polyantha Wight against Bacillus cereus and Bacillus subtilis Lau, Kah Yan Mohd Zainin, Nurul Syazwani Abas, Faridah Rukayadi, Yaya The spore-forming bacteria, Bacillus spp., have often been associated with the contamination of rice and other starchy products. In this study, the extract of Indonesian bay leaf (Eugenia polyantha) was assessed for its antibacterial and sporicidal activities against vegetative cells and spores of Bacillus cereus ATCC33019 as well as Bacillus subtilis ATCC6633. The Minimum Inhibitory Concentration of E. polyantha extract against vegetative cells of B. cereus and B. subtilis were 0.31 and 0.63 mg/mL, respectively, while both their Minimum Bactericidal Concentration were 2.50 mg/mL. Time kill curves demonstrated that the bactericidal endpoint for B. cereus and B. subtilis was reached at concentration of 2.50 and 5.00 mg/mL, respectively (8× MIC) after 4 h of incubation. E. polyantha extract inactivated more than 3-log (99.99%) of B. cereus and B. subtilis spores at a concentration of 10.00 mg/mL (1.0%) after 1 h of incubation and the spores was completely killed at 25.00 mg/mL (2.5%). The sporicidal activity of E. polyantha extract was not affected by different temperatures treatment and alteration of pH. In conclusion, the potential anti-Bacillus activities of E. polyantha extract might support its use as a natural food preservative. Excellent Publishers 2014 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/12929/1/Antibacterial%20and%20sporicidal%20activity%20of%20Eugenia%20polyantha%20Wight%20against%20Bacillus%20cereus%20and%20Bacillus%20subtilis.pdf Lau, Kah Yan and Mohd Zainin, Nurul Syazwani and Abas, Faridah and Rukayadi, Yaya (2014) Antibacterial and sporicidal activity of Eugenia polyantha Wight against Bacillus cereus and Bacillus subtilis. International Journal of Current Microbiology and Applied Sciences, 3 (12). pp. 499-510. ISSN 2319-7692; ESSN: 2319-7706 https://www.ijcmas.com/Archives-25.php |
| spellingShingle | Lau, Kah Yan Mohd Zainin, Nurul Syazwani Abas, Faridah Rukayadi, Yaya Antibacterial and sporicidal activity of Eugenia polyantha Wight against Bacillus cereus and Bacillus subtilis |
| title | Antibacterial and sporicidal activity of Eugenia polyantha Wight against Bacillus cereus and Bacillus subtilis |
| title_full | Antibacterial and sporicidal activity of Eugenia polyantha Wight against Bacillus cereus and Bacillus subtilis |
| title_fullStr | Antibacterial and sporicidal activity of Eugenia polyantha Wight against Bacillus cereus and Bacillus subtilis |
| title_full_unstemmed | Antibacterial and sporicidal activity of Eugenia polyantha Wight against Bacillus cereus and Bacillus subtilis |
| title_short | Antibacterial and sporicidal activity of Eugenia polyantha Wight against Bacillus cereus and Bacillus subtilis |
| title_sort | antibacterial and sporicidal activity of eugenia polyantha wight against bacillus cereus and bacillus subtilis |
| url | http://psasir.upm.edu.my/id/eprint/12929/ http://psasir.upm.edu.my/id/eprint/12929/ http://psasir.upm.edu.my/id/eprint/12929/1/Antibacterial%20and%20sporicidal%20activity%20of%20Eugenia%20polyantha%20Wight%20against%20Bacillus%20cereus%20and%20Bacillus%20subtilis.pdf |