A study on the use of organic additives on the protocorm-like bodies (PLBS) growth of Phalaenopsis violacea orchid

The potential of different concentrations of various banana cultivars extracts, papaya extract, tomato extract and coconut water (0, 10, 20, 30%) to the control (without carbon source and plant growth regulator free medium) for reliable proliferation of Phalenopsis violacea protocorm-like bodies (PL...

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Main Authors: Gnasekaran, Pavallekoodi, Rathinam, Xavier, Sinniah, Uma Rani, Subramaniam, Sreeramanan
Format: Article
Language:English
English
Published: Mohammad Abdul Mushin Agriculture and Engineering Consultants 2010
Online Access:http://psasir.upm.edu.my/id/eprint/12882/
http://psasir.upm.edu.my/id/eprint/12882/1/A%20study%20on%20the%20use%20of%20organic%20additives%20on%20the%20protocorm.pdf
http://psasir.upm.edu.my/id/eprint/12882/2/A%20study%20on%20the%20use%20of%20organic%20additives%20on%20the%20protocorm.pdf
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author Gnasekaran, Pavallekoodi
Rathinam, Xavier
Sinniah, Uma Rani
Subramaniam, Sreeramanan
author_facet Gnasekaran, Pavallekoodi
Rathinam, Xavier
Sinniah, Uma Rani
Subramaniam, Sreeramanan
author_sort Gnasekaran, Pavallekoodi
building UPM Institutional Repository
collection Online Access
description The potential of different concentrations of various banana cultivars extracts, papaya extract, tomato extract and coconut water (0, 10, 20, 30%) to the control (without carbon source and plant growth regulator free medium) for reliable proliferation of Phalenopsis violacea protocorm-like bodies (PLB) under in vitro condition. The results indicated that organic extracts were taken up from the media as shown by the increased in percentage of PLB proliferation rate. Maximum growth of PLB was obtained in half-strength Murashige and Skoog (MS) semi-solid medium supplemented with 10% of Mas (AA) banana pulp extract. However, it has been noticed that at a higher concentrations of organic extract tend to be inhibitory to the PLB proliferation which could be due to the osmotic stress. PLBs grown in half-strength MS supplemented with papaya extract had the lowest PLB proliferation rate among the different organic extracts. It can be concluded that, adding Mas (AA) banana pulp extract at concentration of 10% can be a potential replacement of sucrose in the media. Therefore, the short time length of in vitro culture and its high efficiency makes addition of suitable organic extracts well suited for mass propagation of Phalenopsis violacea orchid.
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language English
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spelling upm-128822015-11-27T08:30:23Z http://psasir.upm.edu.my/id/eprint/12882/ A study on the use of organic additives on the protocorm-like bodies (PLBS) growth of Phalaenopsis violacea orchid Gnasekaran, Pavallekoodi Rathinam, Xavier Sinniah, Uma Rani Subramaniam, Sreeramanan The potential of different concentrations of various banana cultivars extracts, papaya extract, tomato extract and coconut water (0, 10, 20, 30%) to the control (without carbon source and plant growth regulator free medium) for reliable proliferation of Phalenopsis violacea protocorm-like bodies (PLB) under in vitro condition. The results indicated that organic extracts were taken up from the media as shown by the increased in percentage of PLB proliferation rate. Maximum growth of PLB was obtained in half-strength Murashige and Skoog (MS) semi-solid medium supplemented with 10% of Mas (AA) banana pulp extract. However, it has been noticed that at a higher concentrations of organic extract tend to be inhibitory to the PLB proliferation which could be due to the osmotic stress. PLBs grown in half-strength MS supplemented with papaya extract had the lowest PLB proliferation rate among the different organic extracts. It can be concluded that, adding Mas (AA) banana pulp extract at concentration of 10% can be a potential replacement of sucrose in the media. Therefore, the short time length of in vitro culture and its high efficiency makes addition of suitable organic extracts well suited for mass propagation of Phalenopsis violacea orchid. Mohammad Abdul Mushin Agriculture and Engineering Consultants 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12882/1/A%20study%20on%20the%20use%20of%20organic%20additives%20on%20the%20protocorm.pdf application/pdf en http://psasir.upm.edu.my/id/eprint/12882/2/A%20study%20on%20the%20use%20of%20organic%20additives%20on%20the%20protocorm.pdf Gnasekaran, Pavallekoodi and Rathinam, Xavier and Sinniah, Uma Rani and Subramaniam, Sreeramanan (2010) A study on the use of organic additives on the protocorm-like bodies (PLBS) growth of Phalaenopsis violacea orchid. Journal of Phytology, 2 (1). pp. 29-33. ISSN 2075-6240 http://journal-phytology.com/index.php/phyto/article/view/1262
spellingShingle Gnasekaran, Pavallekoodi
Rathinam, Xavier
Sinniah, Uma Rani
Subramaniam, Sreeramanan
A study on the use of organic additives on the protocorm-like bodies (PLBS) growth of Phalaenopsis violacea orchid
title A study on the use of organic additives on the protocorm-like bodies (PLBS) growth of Phalaenopsis violacea orchid
title_full A study on the use of organic additives on the protocorm-like bodies (PLBS) growth of Phalaenopsis violacea orchid
title_fullStr A study on the use of organic additives on the protocorm-like bodies (PLBS) growth of Phalaenopsis violacea orchid
title_full_unstemmed A study on the use of organic additives on the protocorm-like bodies (PLBS) growth of Phalaenopsis violacea orchid
title_short A study on the use of organic additives on the protocorm-like bodies (PLBS) growth of Phalaenopsis violacea orchid
title_sort study on the use of organic additives on the protocorm-like bodies (plbs) growth of phalaenopsis violacea orchid
url http://psasir.upm.edu.my/id/eprint/12882/
http://psasir.upm.edu.my/id/eprint/12882/
http://psasir.upm.edu.my/id/eprint/12882/1/A%20study%20on%20the%20use%20of%20organic%20additives%20on%20the%20protocorm.pdf
http://psasir.upm.edu.my/id/eprint/12882/2/A%20study%20on%20the%20use%20of%20organic%20additives%20on%20the%20protocorm.pdf