A rapid method for determination of commercial β-carotene in RBD palm olein by fourier transform infrared spectroscopy.

A rapid method for the determination of commercial β-carotene in refined bleached and deodorized (RBD) palm olein using Fourier transform infrared (FTIR) spectroscopy was developed. The fifty RBD palm olein samples spiked by a known amount of commercial (30%)β-carotene to produce a wide range of con...

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Main Authors: Ammawath, Wanna, Che Man, Yaakob
Format: Article
Language:English
English
Published: Asian Journal of Food and Agro-Industry 2010
Online Access:http://psasir.upm.edu.my/id/eprint/12804/
http://psasir.upm.edu.my/id/eprint/12804/1/A%20rapid%20method%20for%20determination%20of%20commercial%20%CE%B2.pdf
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author Ammawath, Wanna
Che Man, Yaakob
author_facet Ammawath, Wanna
Che Man, Yaakob
author_sort Ammawath, Wanna
building UPM Institutional Repository
collection Online Access
description A rapid method for the determination of commercial β-carotene in refined bleached and deodorized (RBD) palm olein using Fourier transform infrared (FTIR) spectroscopy was developed. The fifty RBD palm olein samples spiked by a known amount of commercial (30%)β-carotene to produce a wide range of concentrations up to 2000 ppm were used. Samples were separated into two groups for the calibration and validation models. The partial least squares (PLS) calibration models for predicting β-carotene was developed by using the FTIR spectral region at 980-915 cm-1 which is associated with trans double bond CH absorption. The accuracy of the method was comparable to that of the HPLC method with a coefficient of determination (R2) and standard error of calibration (SEC) for commercial β-carotene 0.9934 and 52.29, respectively. The FTIR method developed was shown to be efficient, accurate and suitable for routine quality control analysis for the food industry with results obtainable in about 2.5 min.
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publishDate 2010
publisher Asian Journal of Food and Agro-Industry
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spelling upm-128042015-11-12T07:07:56Z http://psasir.upm.edu.my/id/eprint/12804/ A rapid method for determination of commercial β-carotene in RBD palm olein by fourier transform infrared spectroscopy. Ammawath, Wanna Che Man, Yaakob A rapid method for the determination of commercial β-carotene in refined bleached and deodorized (RBD) palm olein using Fourier transform infrared (FTIR) spectroscopy was developed. The fifty RBD palm olein samples spiked by a known amount of commercial (30%)β-carotene to produce a wide range of concentrations up to 2000 ppm were used. Samples were separated into two groups for the calibration and validation models. The partial least squares (PLS) calibration models for predicting β-carotene was developed by using the FTIR spectral region at 980-915 cm-1 which is associated with trans double bond CH absorption. The accuracy of the method was comparable to that of the HPLC method with a coefficient of determination (R2) and standard error of calibration (SEC) for commercial β-carotene 0.9934 and 52.29, respectively. The FTIR method developed was shown to be efficient, accurate and suitable for routine quality control analysis for the food industry with results obtainable in about 2.5 min. Asian Journal of Food and Agro-Industry 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12804/1/A%20rapid%20method%20for%20determination%20of%20commercial%20%CE%B2.pdf Ammawath, Wanna and Che Man, Yaakob (2010) A rapid method for determination of commercial β-carotene in RBD palm olein by fourier transform infrared spectroscopy. Asian Journal of Food and Agro-Industry, 3 (4). pp. 443-452. ISSN 1906-3040 English
spellingShingle Ammawath, Wanna
Che Man, Yaakob
A rapid method for determination of commercial β-carotene in RBD palm olein by fourier transform infrared spectroscopy.
title A rapid method for determination of commercial β-carotene in RBD palm olein by fourier transform infrared spectroscopy.
title_full A rapid method for determination of commercial β-carotene in RBD palm olein by fourier transform infrared spectroscopy.
title_fullStr A rapid method for determination of commercial β-carotene in RBD palm olein by fourier transform infrared spectroscopy.
title_full_unstemmed A rapid method for determination of commercial β-carotene in RBD palm olein by fourier transform infrared spectroscopy.
title_short A rapid method for determination of commercial β-carotene in RBD palm olein by fourier transform infrared spectroscopy.
title_sort rapid method for determination of commercial β-carotene in rbd palm olein by fourier transform infrared spectroscopy.
url http://psasir.upm.edu.my/id/eprint/12804/
http://psasir.upm.edu.my/id/eprint/12804/1/A%20rapid%20method%20for%20determination%20of%20commercial%20%CE%B2.pdf