A new thermostable lipase by Aneurinibacillus thermoaerophilus strain HZ: nutritional studies
A thermophilic Aneurinibacillus thermoaerophilus strain HZ producing a thermostable lipase was isolated from hot spring in Malaysia. Maximum lipase production byA. thermoaerophilus strain HZ was obtained at pH 7.5 when grown under shaking condition (150 rpm) at 60 °C for 48 h. In order to increase t...
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| Format: | Article |
| Language: | English |
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Springer
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12757/ http://psasir.upm.edu.my/id/eprint/12757/1/A%20new%20thermostable%20lipase%20by%20Aneurinibacillus%20thermoaerophilus%20strain%20HZ.pdf |
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| author | Raja Abdul Rahman, Raja Noor Zaliha Masomian, Malihe Salleh, Abu Bakar Basri, Mahiran |
| author_facet | Raja Abdul Rahman, Raja Noor Zaliha Masomian, Malihe Salleh, Abu Bakar Basri, Mahiran |
| author_sort | Raja Abdul Rahman, Raja Noor Zaliha |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A thermophilic Aneurinibacillus thermoaerophilus strain HZ producing a thermostable lipase was isolated from hot spring in Malaysia. Maximum lipase production byA. thermoaerophilus strain HZ was obtained at pH 7.5 when grown under shaking condition (150 rpm) at 60 °C for 48 h. In order to increase the lipase production, optimization of nutritional factors was studied. Maximum lipase production was obtained in the presence of peptone as the best nitrogen source. Among the various natural and synthetic triglycerides used, olive oil served as the best substrate for production of extracellular lipase. Additional carbon sources added in this study did not significantly increase the lipase production while metal ions, Mg2+, Na+, Ca2+ and K+ were found to enhance lipase production. In addition, lipase production was stimulated by Tween 85 as a surfactant. |
| first_indexed | 2025-11-15T07:50:56Z |
| format | Article |
| id | upm-12757 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:50:56Z |
| publishDate | 2009 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-127572016-09-26T09:11:06Z http://psasir.upm.edu.my/id/eprint/12757/ A new thermostable lipase by Aneurinibacillus thermoaerophilus strain HZ: nutritional studies Raja Abdul Rahman, Raja Noor Zaliha Masomian, Malihe Salleh, Abu Bakar Basri, Mahiran A thermophilic Aneurinibacillus thermoaerophilus strain HZ producing a thermostable lipase was isolated from hot spring in Malaysia. Maximum lipase production byA. thermoaerophilus strain HZ was obtained at pH 7.5 when grown under shaking condition (150 rpm) at 60 °C for 48 h. In order to increase the lipase production, optimization of nutritional factors was studied. Maximum lipase production was obtained in the presence of peptone as the best nitrogen source. Among the various natural and synthetic triglycerides used, olive oil served as the best substrate for production of extracellular lipase. Additional carbon sources added in this study did not significantly increase the lipase production while metal ions, Mg2+, Na+, Ca2+ and K+ were found to enhance lipase production. In addition, lipase production was stimulated by Tween 85 as a surfactant. Springer 2009-03-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12757/1/A%20new%20thermostable%20lipase%20by%20Aneurinibacillus%20thermoaerophilus%20strain%20HZ.pdf Raja Abdul Rahman, Raja Noor Zaliha and Masomian, Malihe and Salleh, Abu Bakar and Basri, Mahiran (2009) A new thermostable lipase by Aneurinibacillus thermoaerophilus strain HZ: nutritional studies. Annals of Microbiology, 59 (1). pp. 133-139. ISSN 1590-4261; ESSN: 1869-2044 http://link.springer.com/article/10.1007/BF03175610?view=classic 10.1007/BF03175610 |
| spellingShingle | Raja Abdul Rahman, Raja Noor Zaliha Masomian, Malihe Salleh, Abu Bakar Basri, Mahiran A new thermostable lipase by Aneurinibacillus thermoaerophilus strain HZ: nutritional studies |
| title | A new thermostable lipase by Aneurinibacillus thermoaerophilus strain HZ: nutritional studies |
| title_full | A new thermostable lipase by Aneurinibacillus thermoaerophilus strain HZ: nutritional studies |
| title_fullStr | A new thermostable lipase by Aneurinibacillus thermoaerophilus strain HZ: nutritional studies |
| title_full_unstemmed | A new thermostable lipase by Aneurinibacillus thermoaerophilus strain HZ: nutritional studies |
| title_short | A new thermostable lipase by Aneurinibacillus thermoaerophilus strain HZ: nutritional studies |
| title_sort | new thermostable lipase by aneurinibacillus thermoaerophilus strain hz: nutritional studies |
| url | http://psasir.upm.edu.my/id/eprint/12757/ http://psasir.upm.edu.my/id/eprint/12757/ http://psasir.upm.edu.my/id/eprint/12757/ http://psasir.upm.edu.my/id/eprint/12757/1/A%20new%20thermostable%20lipase%20by%20Aneurinibacillus%20thermoaerophilus%20strain%20HZ.pdf |