Improved NARP-HPLC method for separating triglycerides of palm olein and its solid fractions obtained at low temperature storage
Non-aqueous reversed-phase high-performance liquid chromatography (NARP-HPLC) with refractive index (RI) detection is described and used for palm olein and its fractions obtained at 12.5 °C for 12–24 h. The calculation formula for fatty acid methyl esters (FAMEs) and carbon number (CN) from the data...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
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Elsevier
1996
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12755/ http://psasir.upm.edu.my/id/eprint/12755/1/Improved%20NARP-HPLC%20method%20for%20separating%20triglycerides%20of%20palm%20olein%20and%20its%20solid%20fractions%20obtained%20at%20low%20temperature%20storage.pdf http://psasir.upm.edu.my/id/eprint/12755/7/1-s2.0-0308814695001786-main.pdf |
| _version_ | 1848841921937539072 |
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| author | Phyo, Zaw Swe Che Man, Yaakob Mohd Ghazali, Hasanah |
| author_facet | Phyo, Zaw Swe Che Man, Yaakob Mohd Ghazali, Hasanah |
| author_sort | Phyo, Zaw Swe |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Non-aqueous reversed-phase high-performance liquid chromatography (NARP-HPLC) with refractive index (RI) detection is described and used for palm olein and its fractions obtained at 12.5 °C for 12–24 h. The calculation formula for fatty acid methyl esters (FAMEs) and carbon number (CN) from the data obtained by NARP-HPLC is described and correction factors for all carbon numbers and fatty acids are tabulated. The results were compared with those obtained from FAMEs analysed on 10% SP 2330 Supelco packed column gas-liquid chromatography (GLC) and CN analysed on 3% OV-1 Supelco packed column GLC. The results were found to agree well for C48, C50, C52, C16:0, C18:0 and C18:1 (correction factor ≈ 1.0); however, a slight variation was observed for components C54, C14:0 and C18:2 (correction factor 1.0 ± 0.37). |
| first_indexed | 2025-11-15T07:50:55Z |
| format | Article |
| id | upm-12755 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:50:55Z |
| publishDate | 1996 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-127552024-08-05T08:22:57Z http://psasir.upm.edu.my/id/eprint/12755/ Improved NARP-HPLC method for separating triglycerides of palm olein and its solid fractions obtained at low temperature storage Phyo, Zaw Swe Che Man, Yaakob Mohd Ghazali, Hasanah Non-aqueous reversed-phase high-performance liquid chromatography (NARP-HPLC) with refractive index (RI) detection is described and used for palm olein and its fractions obtained at 12.5 °C for 12–24 h. The calculation formula for fatty acid methyl esters (FAMEs) and carbon number (CN) from the data obtained by NARP-HPLC is described and correction factors for all carbon numbers and fatty acids are tabulated. The results were compared with those obtained from FAMEs analysed on 10% SP 2330 Supelco packed column gas-liquid chromatography (GLC) and CN analysed on 3% OV-1 Supelco packed column GLC. The results were found to agree well for C48, C50, C52, C16:0, C18:0 and C18:1 (correction factor ≈ 1.0); however, a slight variation was observed for components C54, C14:0 and C18:2 (correction factor 1.0 ± 0.37). Elsevier 1996-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/12755/1/Improved%20NARP-HPLC%20method%20for%20separating%20triglycerides%20of%20palm%20olein%20and%20its%20solid%20fractions%20obtained%20at%20low%20temperature%20storage.pdf text en http://psasir.upm.edu.my/id/eprint/12755/7/1-s2.0-0308814695001786-main.pdf Phyo, Zaw Swe and Che Man, Yaakob and Mohd Ghazali, Hasanah (1996) Improved NARP-HPLC method for separating triglycerides of palm olein and its solid fractions obtained at low temperature storage. Food Chemistry, 56 (2). pp. 181-186. ISSN 0308-8146; ESSN: 1873-7072 http://www.sciencedirect.com/science/article/pii/0308814695001786 10.1016/0308-8146(95)00178-6 |
| spellingShingle | Phyo, Zaw Swe Che Man, Yaakob Mohd Ghazali, Hasanah Improved NARP-HPLC method for separating triglycerides of palm olein and its solid fractions obtained at low temperature storage |
| title | Improved NARP-HPLC method for separating triglycerides of palm olein and its solid fractions obtained at low temperature storage |
| title_full | Improved NARP-HPLC method for separating triglycerides of palm olein and its solid fractions obtained at low temperature storage |
| title_fullStr | Improved NARP-HPLC method for separating triglycerides of palm olein and its solid fractions obtained at low temperature storage |
| title_full_unstemmed | Improved NARP-HPLC method for separating triglycerides of palm olein and its solid fractions obtained at low temperature storage |
| title_short | Improved NARP-HPLC method for separating triglycerides of palm olein and its solid fractions obtained at low temperature storage |
| title_sort | improved narp-hplc method for separating triglycerides of palm olein and its solid fractions obtained at low temperature storage |
| url | http://psasir.upm.edu.my/id/eprint/12755/ http://psasir.upm.edu.my/id/eprint/12755/ http://psasir.upm.edu.my/id/eprint/12755/ http://psasir.upm.edu.my/id/eprint/12755/1/Improved%20NARP-HPLC%20method%20for%20separating%20triglycerides%20of%20palm%20olein%20and%20its%20solid%20fractions%20obtained%20at%20low%20temperature%20storage.pdf http://psasir.upm.edu.my/id/eprint/12755/7/1-s2.0-0308814695001786-main.pdf |