A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria
In this research, we modify a previously developed assay for the quantification molybdenum blue to determine whether inhibitors to molybdate reduction in bacteria inhibits cellular reduction or inhibit the chemical formation of one of the intermediate of molybdenum blue; phosphomolybdate. We manage...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Asian Network for Scientific Information
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12722/ http://psasir.upm.edu.my/id/eprint/12722/1/A%20method%20to%20study%20the%20effects%20of%20chemical%20and%20biological%20reduction%20of%20molybdate%20to%20molybdenum%20blue%20in%20bacteria.pdf |
| _version_ | 1848841912850579456 |
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| author | Abd. Shukor, Mohd. Yunus Shamsuddin, Burhanuddin Mohamad, Othman Ithnin, Khalid Shamaan, Nor Aripin Syed, Mohd. Arif |
| author_facet | Abd. Shukor, Mohd. Yunus Shamsuddin, Burhanuddin Mohamad, Othman Ithnin, Khalid Shamaan, Nor Aripin Syed, Mohd. Arif |
| author_sort | Abd. Shukor, Mohd. Yunus |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this research, we modify a previously developed assay for the quantification molybdenum blue to determine whether inhibitors to molybdate reduction in bacteria inhibits cellular reduction or inhibit the chemical formation of one of the intermediate of molybdenum blue; phosphomolybdate. We manage to prove that inhibition of molybdate reduction by phosphate and arsenate is at the level of phosphomolybdate and not cellular. We also prove that mercury is a physiological inhibitor to molybdate reduction. We suggest the use of this method to assess the effect of inhibitors and activators to molybdate reduction in bacteria. |
| first_indexed | 2025-11-15T07:50:47Z |
| format | Article |
| id | upm-12722 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:50:47Z |
| publishDate | 2008 |
| publisher | Asian Network for Scientific Information |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-127222015-12-02T03:29:01Z http://psasir.upm.edu.my/id/eprint/12722/ A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria Abd. Shukor, Mohd. Yunus Shamsuddin, Burhanuddin Mohamad, Othman Ithnin, Khalid Shamaan, Nor Aripin Syed, Mohd. Arif In this research, we modify a previously developed assay for the quantification molybdenum blue to determine whether inhibitors to molybdate reduction in bacteria inhibits cellular reduction or inhibit the chemical formation of one of the intermediate of molybdenum blue; phosphomolybdate. We manage to prove that inhibition of molybdate reduction by phosphate and arsenate is at the level of phosphomolybdate and not cellular. We also prove that mercury is a physiological inhibitor to molybdate reduction. We suggest the use of this method to assess the effect of inhibitors and activators to molybdate reduction in bacteria. Asian Network for Scientific Information 2008-02-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12722/1/A%20method%20to%20study%20the%20effects%20of%20chemical%20and%20biological%20reduction%20of%20molybdate%20to%20molybdenum%20blue%20in%20bacteria.pdf Abd. Shukor, Mohd. Yunus and Shamsuddin, Burhanuddin and Mohamad, Othman and Ithnin, Khalid and Shamaan, Nor Aripin and Syed, Mohd. Arif (2008) A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria. Pakistan Journal of Biological Sciences, 11 (4). pp. 672-675. ISSN 1028-8880; ESSN: 1812-5735 http://scialert.net/abstract/?doi=pjbs.2008.672.675 10.3923/pjbs.2008.672.675 |
| spellingShingle | Abd. Shukor, Mohd. Yunus Shamsuddin, Burhanuddin Mohamad, Othman Ithnin, Khalid Shamaan, Nor Aripin Syed, Mohd. Arif A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria |
| title | A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria |
| title_full | A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria |
| title_fullStr | A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria |
| title_full_unstemmed | A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria |
| title_short | A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria |
| title_sort | method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria |
| url | http://psasir.upm.edu.my/id/eprint/12722/ http://psasir.upm.edu.my/id/eprint/12722/ http://psasir.upm.edu.my/id/eprint/12722/ http://psasir.upm.edu.my/id/eprint/12722/1/A%20method%20to%20study%20the%20effects%20of%20chemical%20and%20biological%20reduction%20of%20molybdate%20to%20molybdenum%20blue%20in%20bacteria.pdf |