A comparative study of Metallothionein gene expression in peripheral lymphocytes and blood cadmium level among die casting male workers
Cadmium were found in the die casting factory as by product of zinc alloy that used in the manufacturing of die cast. Metallothionein (MT) a carrier protein plays an important role in the detoxification process of cadmium in human. The usefulness of MT gene expression in peripheral blood lymphocytes...
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Canadian Center of Science and Education
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12664/ |
| _version_ | 1848841896276787200 |
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| author | Ooi, Keok Loo Adon, Mohd Yusoff Cheah, Yoke Kqueen Ismail, Patimah Mat Jais, Abdul Manan |
| author_facet | Ooi, Keok Loo Adon, Mohd Yusoff Cheah, Yoke Kqueen Ismail, Patimah Mat Jais, Abdul Manan |
| author_sort | Ooi, Keok Loo |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Cadmium were found in the die casting factory as by product of zinc alloy that used in the manufacturing of die cast. Metallothionein (MT) a carrier protein plays an important role in the detoxification process of cadmium in human. The usefulness of MT gene expression in peripheral blood lymphocytes (PBLs) as a biomarker of cadmium exposure and susceptibility could be determine by reverse transcriptase–polymerase chain reaction (RT-PCR). 41 male workers from die casting factory were involved in the cross-sectional comparative study and were divided into exposed and comparative group. MT gene expressions were found to proportionates increase with blood cadmium (BCd) levels. MT basal expression levels were significantly correlated with the BCd levels with r-value 0.616 for exposed group and 0.639 for comparative group. MT induction expression level were significant correlated with BCd level (r = 0.188 for exposed group, r = 0.342 for comparative group). This
suggested that MT gene expression in PBLs can be used as a biomarker of susceptibility to cadmium. |
| first_indexed | 2025-11-15T07:50:31Z |
| format | Article |
| id | upm-12664 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T07:50:31Z |
| publishDate | 2010 |
| publisher | Canadian Center of Science and Education |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-126642015-05-29T04:34:20Z http://psasir.upm.edu.my/id/eprint/12664/ A comparative study of Metallothionein gene expression in peripheral lymphocytes and blood cadmium level among die casting male workers Ooi, Keok Loo Adon, Mohd Yusoff Cheah, Yoke Kqueen Ismail, Patimah Mat Jais, Abdul Manan Cadmium were found in the die casting factory as by product of zinc alloy that used in the manufacturing of die cast. Metallothionein (MT) a carrier protein plays an important role in the detoxification process of cadmium in human. The usefulness of MT gene expression in peripheral blood lymphocytes (PBLs) as a biomarker of cadmium exposure and susceptibility could be determine by reverse transcriptase–polymerase chain reaction (RT-PCR). 41 male workers from die casting factory were involved in the cross-sectional comparative study and were divided into exposed and comparative group. MT gene expressions were found to proportionates increase with blood cadmium (BCd) levels. MT basal expression levels were significantly correlated with the BCd levels with r-value 0.616 for exposed group and 0.639 for comparative group. MT induction expression level were significant correlated with BCd level (r = 0.188 for exposed group, r = 0.342 for comparative group). This suggested that MT gene expression in PBLs can be used as a biomarker of susceptibility to cadmium. Canadian Center of Science and Education 2010-10 Article PeerReviewed Ooi, Keok Loo and Adon, Mohd Yusoff and Cheah, Yoke Kqueen and Ismail, Patimah and Mat Jais, Abdul Manan (2010) A comparative study of Metallothionein gene expression in peripheral lymphocytes and blood cadmium level among die casting male workers. Global Journal of Health Science, 2 (2). pp. 129-136. ISSN 1916-9736; ESSN: 1916-9744 http://www.ccsenet.org/journal/index.php/gjhs/article/view/5162 10.5539/gjhs.v2n2p129 |
| spellingShingle | Ooi, Keok Loo Adon, Mohd Yusoff Cheah, Yoke Kqueen Ismail, Patimah Mat Jais, Abdul Manan A comparative study of Metallothionein gene expression in peripheral lymphocytes and blood cadmium level among die casting male workers |
| title | A comparative study of Metallothionein gene expression in peripheral lymphocytes and blood cadmium level among die casting male workers |
| title_full | A comparative study of Metallothionein gene expression in peripheral lymphocytes and blood cadmium level among die casting male workers |
| title_fullStr | A comparative study of Metallothionein gene expression in peripheral lymphocytes and blood cadmium level among die casting male workers |
| title_full_unstemmed | A comparative study of Metallothionein gene expression in peripheral lymphocytes and blood cadmium level among die casting male workers |
| title_short | A comparative study of Metallothionein gene expression in peripheral lymphocytes and blood cadmium level among die casting male workers |
| title_sort | comparative study of metallothionein gene expression in peripheral lymphocytes and blood cadmium level among die casting male workers |
| url | http://psasir.upm.edu.my/id/eprint/12664/ http://psasir.upm.edu.my/id/eprint/12664/ http://psasir.upm.edu.my/id/eprint/12664/ |