Host range evaluation and morphological characterization of Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew in Malaysia

A total of 29 isolates of Pseudoperonospora cubensis were collected from various cucurbit farms in West Malaysia. Sporangia of 13 isolates had the ability to germinate at 14°C and were used for host range (pathotype) study using leaf disc assay on a set of twelve cucurbit cultivars. Twelve differen...

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Main Authors: Salati, Mansour, Wong, Mui Yun, Meon, Sariah, Nik Hassan, Nik Masdek
Format: Article
Language:English
Published: Academic Journals 2010
Online Access:http://psasir.upm.edu.my/id/eprint/12404/
http://psasir.upm.edu.my/id/eprint/12404/1/Host_range.pdf
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author Salati, Mansour
Wong, Mui Yun
Meon, Sariah
Nik Hassan, Nik Masdek
author_facet Salati, Mansour
Wong, Mui Yun
Meon, Sariah
Nik Hassan, Nik Masdek
author_sort Salati, Mansour
building UPM Institutional Repository
collection Online Access
description A total of 29 isolates of Pseudoperonospora cubensis were collected from various cucurbit farms in West Malaysia. Sporangia of 13 isolates had the ability to germinate at 14°C and were used for host range (pathotype) study using leaf disc assay on a set of twelve cucurbit cultivars. Twelve different pathotypes of P. cubensis were determined and this demonstrated that P. cubensis isolates from cucurbit farms in West Malaysia are highly variable. Based on the host range study, majority of P. cubensis isolates were categorized into medium and high pathogenicity groupings and this fact shows the potential of this pathogen in invading cucurbit fields in the tropical regions. However, the pathotypes could not be differentiated based on morphological characterization of the sporangia and sporangiophores. Therefore, molecular characterization of the 13 isolates of P. cubensis will be useful to study the relationships among them.
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spelling upm-124042019-10-21T06:49:53Z http://psasir.upm.edu.my/id/eprint/12404/ Host range evaluation and morphological characterization of Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew in Malaysia Salati, Mansour Wong, Mui Yun Meon, Sariah Nik Hassan, Nik Masdek A total of 29 isolates of Pseudoperonospora cubensis were collected from various cucurbit farms in West Malaysia. Sporangia of 13 isolates had the ability to germinate at 14°C and were used for host range (pathotype) study using leaf disc assay on a set of twelve cucurbit cultivars. Twelve different pathotypes of P. cubensis were determined and this demonstrated that P. cubensis isolates from cucurbit farms in West Malaysia are highly variable. Based on the host range study, majority of P. cubensis isolates were categorized into medium and high pathogenicity groupings and this fact shows the potential of this pathogen in invading cucurbit fields in the tropical regions. However, the pathotypes could not be differentiated based on morphological characterization of the sporangia and sporangiophores. Therefore, molecular characterization of the 13 isolates of P. cubensis will be useful to study the relationships among them. Academic Journals 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12404/1/Host_range.pdf Salati, Mansour and Wong, Mui Yun and Meon, Sariah and Nik Hassan, Nik Masdek (2010) Host range evaluation and morphological characterization of Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew in Malaysia. African Journal of Biotechnology, 9 (31). art. no. B8D9C1323921. pp. 4897-4903. ISSN 1684-5315 http://www.academicjournals.org/journal/AJB/article-abstract/B8D9C1323921 10.5897/AJB09.1922
spellingShingle Salati, Mansour
Wong, Mui Yun
Meon, Sariah
Nik Hassan, Nik Masdek
Host range evaluation and morphological characterization of Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew in Malaysia
title Host range evaluation and morphological characterization of Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew in Malaysia
title_full Host range evaluation and morphological characterization of Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew in Malaysia
title_fullStr Host range evaluation and morphological characterization of Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew in Malaysia
title_full_unstemmed Host range evaluation and morphological characterization of Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew in Malaysia
title_short Host range evaluation and morphological characterization of Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew in Malaysia
title_sort host range evaluation and morphological characterization of pseudoperonospora cubensis, the causal agent of cucurbit downy mildew in malaysia
url http://psasir.upm.edu.my/id/eprint/12404/
http://psasir.upm.edu.my/id/eprint/12404/
http://psasir.upm.edu.my/id/eprint/12404/
http://psasir.upm.edu.my/id/eprint/12404/1/Host_range.pdf