Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis)
Chromosome analysis was undertaken on Murrah,swamp , F1 (Murrah x swamp ) and backcross (F1 female x Murrah male) buffaloes (Bubalus bubalis) using a modified leucocyte culture method combined with Giemsa (G) constitutive heterochromatin (C) and nucleolus organiser region (NOR) banding procedure...
| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
1984
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/12263/ http://psasir.upm.edu.my/id/eprint/12263/1/FPV_1984_3_.pdf |
| _version_ | 1848841796688281600 |
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| author | Abdullah, Mohamad Hilmi |
| author_facet | Abdullah, Mohamad Hilmi |
| author_sort | Abdullah, Mohamad Hilmi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Chromosome analysis was undertaken on Murrah,swamp , F1
(Murrah x swamp ) and backcross (F1 female x Murrah male) buffaloes
(Bubalus bubalis) using a modified leucocyte culture method
combined with Giemsa (G) constitutive heterochromatin (C) and
nucleolus organiser region (NOR) banding procedures . A combination
of 80% medium RPMI 1640 , 20% calf serum , pokeweed mitogen and buffy
coat gave many good quality metaphases . The Murrah , swamp and F1
buffaloes had chromosome complement s of 2n = 50 , 2n = 48 and
2n = 49 respectively . The Murrah karyotype had 5 pairs of submetacentric
chromosome s and 20 pairs of acrocentric chromosomes while
the swamp karyotype had 1 pair of large metacentric chromosomes , 4 submetacentric pairs and 19 pairs of acrocentric chromosomes. /F1
hybrid had an intermediate karyotype. Nine backcross animals had a
2n = 49 complement while 3 backcross animals had a 2n = 50 complement. |
| first_indexed | 2025-11-15T07:48:56Z |
| format | Thesis |
| id | upm-12263 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:48:56Z |
| publishDate | 1984 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-122632024-07-10T08:04:31Z http://psasir.upm.edu.my/id/eprint/12263/ Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis) Abdullah, Mohamad Hilmi Chromosome analysis was undertaken on Murrah,swamp , F1 (Murrah x swamp ) and backcross (F1 female x Murrah male) buffaloes (Bubalus bubalis) using a modified leucocyte culture method combined with Giemsa (G) constitutive heterochromatin (C) and nucleolus organiser region (NOR) banding procedures . A combination of 80% medium RPMI 1640 , 20% calf serum , pokeweed mitogen and buffy coat gave many good quality metaphases . The Murrah , swamp and F1 buffaloes had chromosome complement s of 2n = 50 , 2n = 48 and 2n = 49 respectively . The Murrah karyotype had 5 pairs of submetacentric chromosome s and 20 pairs of acrocentric chromosomes while the swamp karyotype had 1 pair of large metacentric chromosomes , 4 submetacentric pairs and 19 pairs of acrocentric chromosomes. /F1 hybrid had an intermediate karyotype. Nine backcross animals had a 2n = 49 complement while 3 backcross animals had a 2n = 50 complement. 1984-02 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/12263/1/FPV_1984_3_.pdf Abdullah, Mohamad Hilmi (1984) Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis). Doctoral thesis, Universiti Pertanian Malaysia. Water buffalo - Cytogenetics. English |
| spellingShingle | Water buffalo - Cytogenetics. Abdullah, Mohamad Hilmi Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis) |
| title | Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis) |
| title_full | Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis) |
| title_fullStr | Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis) |
| title_full_unstemmed | Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis) |
| title_short | Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis) |
| title_sort | cytogenetic studies on the water buffalo (bubalus bubalis) |
| topic | Water buffalo - Cytogenetics. |
| url | http://psasir.upm.edu.my/id/eprint/12263/ http://psasir.upm.edu.my/id/eprint/12263/1/FPV_1984_3_.pdf |