Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column
The main parameters which influence the behaviour of phase separation in a single-stage Kühni-type aqueous two-phase extraction column containing polyethylene (PEG) and di-potassium hydrogen phosphate were characterised. Two aqueous two-phase system (ATPS) composed of 12% (w/w) PEG 1450 and 12% (w/w...
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| Format: | Article |
| Language: | English English |
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2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12125/ http://psasir.upm.edu.my/id/eprint/12125/1/Physical%20characterisations%20of%20a%20single.pdf |
| _version_ | 1848841758985682944 |
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| author | Luechau, Frank Tau, Chuan Ling Lyddiatt, Andrew |
| author_facet | Luechau, Frank Tau, Chuan Ling Lyddiatt, Andrew |
| author_sort | Luechau, Frank |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The main parameters which influence the behaviour of phase separation in a single-stage Kühni-type aqueous two-phase extraction column containing polyethylene (PEG) and di-potassium hydrogen phosphate were characterised. Two aqueous two-phase system (ATPS) composed of 12% (w/w) PEG 1450 and 12% (w/w) di-potassium hydrogen phosphate (designated as 12/12) and 12% (w/w) PEG 1450 and 11% (w/w) di-potassium hydrogen phosphate (designated as 12/11) were chosen in this study. The hold-up D increased with increasing impeller speeds and mobile phase flow rates. Phase separation for the 12/11 system was slower than that for the 12/12 system, which resulted in higher dispersed phase hold-up values for the 12/11 system. For 12/12 system, mass transfer of plasmid DNA (pDNA) from the dispersed mobile phase to the stationary phase increased rapidly with increasing impeller speeds of 130, 160 and 200 rpm which was reflected in the decreased values for CT/CTo. The degree of back-mixing quantified by the axial dispersion coefficient Dax was estimated to be 2.7 × 10−6 m2 s−1.
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| first_indexed | 2025-11-15T07:48:20Z |
| format | Article |
| id | upm-12125 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:48:20Z |
| publishDate | 2010 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-121252015-10-05T03:12:11Z http://psasir.upm.edu.my/id/eprint/12125/ Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column Luechau, Frank Tau, Chuan Ling Lyddiatt, Andrew The main parameters which influence the behaviour of phase separation in a single-stage Kühni-type aqueous two-phase extraction column containing polyethylene (PEG) and di-potassium hydrogen phosphate were characterised. Two aqueous two-phase system (ATPS) composed of 12% (w/w) PEG 1450 and 12% (w/w) di-potassium hydrogen phosphate (designated as 12/12) and 12% (w/w) PEG 1450 and 11% (w/w) di-potassium hydrogen phosphate (designated as 12/11) were chosen in this study. The hold-up D increased with increasing impeller speeds and mobile phase flow rates. Phase separation for the 12/11 system was slower than that for the 12/12 system, which resulted in higher dispersed phase hold-up values for the 12/11 system. For 12/12 system, mass transfer of plasmid DNA (pDNA) from the dispersed mobile phase to the stationary phase increased rapidly with increasing impeller speeds of 130, 160 and 200 rpm which was reflected in the decreased values for CT/CTo. The degree of back-mixing quantified by the axial dispersion coefficient Dax was estimated to be 2.7 × 10−6 m2 s−1. 2010-07-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12125/1/Physical%20characterisations%20of%20a%20single.pdf Luechau, Frank and Tau, Chuan Ling and Lyddiatt, Andrew (2010) Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column. Biochemical Engineering Journal, 50 (3). pp. 90-98. ISSN 1369-703X http://dx.doi.org/10.1016/j.bej.2010.02.010 English |
| spellingShingle | Luechau, Frank Tau, Chuan Ling Lyddiatt, Andrew Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column |
| title | Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column |
| title_full | Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column |
| title_fullStr | Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column |
| title_full_unstemmed | Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column |
| title_short | Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column |
| title_sort | physical characterisations of a single-stage kühni-type aqueous two-phase extraction column |
| url | http://psasir.upm.edu.my/id/eprint/12125/ http://psasir.upm.edu.my/id/eprint/12125/ http://psasir.upm.edu.my/id/eprint/12125/1/Physical%20characterisations%20of%20a%20single.pdf |