Bio-prospecting locally relevant industrial yeast strains from fermented carbohydrate-rich food waste from Nigeria

Nigerian fermented food is characterized by high carbohydrate concentration. Widely spread yeast strains in the local fermented food waste amass traits useful to biofuel industry. This study aims to identify, characterize and assess the level of stress tolerance of local yeast strains residing in fo...

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Main Authors: Kazeem, Muinat Olanike, Olabamiji, Samson Oluwatosin, Ajijolakewu, Kamoldeen A., Hafid, Halimatun Saadiah
Format: Article
Language:English
Published: University of Baghdad-College of Science 2025
Online Access:http://psasir.upm.edu.my/id/eprint/121191/
http://psasir.upm.edu.my/id/eprint/121191/1/121191.pdf
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author Kazeem, Muinat Olanike
Olabamiji, Samson Oluwatosin
Ajijolakewu, Kamoldeen A.
Hafid, Halimatun Saadiah
author_facet Kazeem, Muinat Olanike
Olabamiji, Samson Oluwatosin
Ajijolakewu, Kamoldeen A.
Hafid, Halimatun Saadiah
author_sort Kazeem, Muinat Olanike
building UPM Institutional Repository
collection Online Access
description Nigerian fermented food is characterized by high carbohydrate concentration. Widely spread yeast strains in the local fermented food waste amass traits useful to biofuel industry. This study aims to identify, characterize and assess the level of stress tolerance of local yeast strains residing in food waste produced from Nigerian "fufu," a fermented cassava product. Through the use of morphological, cellular, and amplification of TS1/ITS4 and D1/D2 rDNA sequencing, the yeast strains were identified. To test the yeast strains' resilience to stress, different concentrations of ethanol (15% - 20%v/v), sugar (5% - 25%w/v), and temperatures (30°C - 45°C) were applied. Out of the eight yeast strains, the isolate Y4, also known as Kazachstania pseudohumilis Y4 (Accession number: ON876535:1), was most promising since it could withstand ethanol at 15% and 20% (v/v). The strain showed tolerance to glucose at 20% (w/v) and temperatures (30°C-45°C). The carbohydrate-rich fermented food waste was a valuable source of stress-tolerant yeast, a vital resource for the bioethanol industry.
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spelling upm-1211912025-10-29T00:40:00Z http://psasir.upm.edu.my/id/eprint/121191/ Bio-prospecting locally relevant industrial yeast strains from fermented carbohydrate-rich food waste from Nigeria Kazeem, Muinat Olanike Olabamiji, Samson Oluwatosin Ajijolakewu, Kamoldeen A. Hafid, Halimatun Saadiah Nigerian fermented food is characterized by high carbohydrate concentration. Widely spread yeast strains in the local fermented food waste amass traits useful to biofuel industry. This study aims to identify, characterize and assess the level of stress tolerance of local yeast strains residing in food waste produced from Nigerian "fufu," a fermented cassava product. Through the use of morphological, cellular, and amplification of TS1/ITS4 and D1/D2 rDNA sequencing, the yeast strains were identified. To test the yeast strains' resilience to stress, different concentrations of ethanol (15% - 20%v/v), sugar (5% - 25%w/v), and temperatures (30°C - 45°C) were applied. Out of the eight yeast strains, the isolate Y4, also known as Kazachstania pseudohumilis Y4 (Accession number: ON876535:1), was most promising since it could withstand ethanol at 15% and 20% (v/v). The strain showed tolerance to glucose at 20% (w/v) and temperatures (30°C-45°C). The carbohydrate-rich fermented food waste was a valuable source of stress-tolerant yeast, a vital resource for the bioethanol industry. University of Baghdad-College of Science 2025-01-30 Article PeerReviewed text en cc_by_nc_4 http://psasir.upm.edu.my/id/eprint/121191/1/121191.pdf Kazeem, Muinat Olanike and Olabamiji, Samson Oluwatosin and Ajijolakewu, Kamoldeen A. and Hafid, Halimatun Saadiah (2025) Bio-prospecting locally relevant industrial yeast strains from fermented carbohydrate-rich food waste from Nigeria. Iraqi Journal of Science, 66 (1). pp. 90-102. ISSN 0067-2904; eISSN: 2312-1637 https://ijs.uobaghdad.edu.iq/index.php/eijs/article/view/10548 10.24996/ijs.2025.66.1.8
spellingShingle Kazeem, Muinat Olanike
Olabamiji, Samson Oluwatosin
Ajijolakewu, Kamoldeen A.
Hafid, Halimatun Saadiah
Bio-prospecting locally relevant industrial yeast strains from fermented carbohydrate-rich food waste from Nigeria
title Bio-prospecting locally relevant industrial yeast strains from fermented carbohydrate-rich food waste from Nigeria
title_full Bio-prospecting locally relevant industrial yeast strains from fermented carbohydrate-rich food waste from Nigeria
title_fullStr Bio-prospecting locally relevant industrial yeast strains from fermented carbohydrate-rich food waste from Nigeria
title_full_unstemmed Bio-prospecting locally relevant industrial yeast strains from fermented carbohydrate-rich food waste from Nigeria
title_short Bio-prospecting locally relevant industrial yeast strains from fermented carbohydrate-rich food waste from Nigeria
title_sort bio-prospecting locally relevant industrial yeast strains from fermented carbohydrate-rich food waste from nigeria
url http://psasir.upm.edu.my/id/eprint/121191/
http://psasir.upm.edu.my/id/eprint/121191/
http://psasir.upm.edu.my/id/eprint/121191/
http://psasir.upm.edu.my/id/eprint/121191/1/121191.pdf