Antimicrobial resistance of zoonotic bacteria isolated from shrimp aquaculture environments in Selangor, Malaysia
Intensive aquaculture farming has caused an increase in antimicrobial use (AMU) and the emergence of antimicrobial resistance (AMR). Malaysia is the ninth-largest shrimp exporter in the world. Nonetheless, the available data on AMR in shrimp aquaculture is insufficient to support efforts to combat t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier B.V.
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/121177/ http://psasir.upm.edu.my/id/eprint/121177/1/121177.pdf |
| _version_ | 1848868329160179712 |
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| author | Devadas, S. Zakaria, Z. Shariff, M. Bhassu, S. Karim, M. Natrah, I. |
| author_facet | Devadas, S. Zakaria, Z. Shariff, M. Bhassu, S. Karim, M. Natrah, I. |
| author_sort | Devadas, S. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Intensive aquaculture farming has caused an increase in antimicrobial use (AMU) and the emergence of antimicrobial resistance (AMR). Malaysia is the ninth-largest shrimp exporter in the world. Nonetheless, the available data on AMR in shrimp aquaculture is insufficient to support efforts to combat the issue effectively. This study analyses the antimicrobial susceptibility profile and multiple antibiotic resistance (MAR) indexes of Vibrio parahaemolyticus, Escherichia coli and Salmonella in shrimp aquaculture environments in Selangor State. In total, 47 bacterial isolates, consisting of 33 (70.2 %) V. parahaemolyticus, 12 (25.5 %) E. coli and two (4.3 %) Salmonella, were isolated. Other than the intrinsically resistant-related classes of antibiotics, the V. parahaemolyticus isolates showed the top five highest percentages of resistance towards ampicillin (81.8 %, n = 27), followed by erythromycin (69.7 %, n = 23), neomycin (60.6 %, n = 20), kanamycin (54.5 %, n = 18) and ciprofloxacin (39.4 %, n = 13). Likewise, the E. coli showed resistance towards ampicillin (66.7 %, n = 8), followed by 58.3 % (n = 7) to azithromycin and cephalexin and 41.7 % (n = 5) to tetracycline, sulfamethoxazole/trimethoprim and neomycin. Thirty (63.8 %) isolates exhibited a MAR index >0.2, while 31.9 % (n = 15) were classified as multidrug-resistant (MDR) and demonstrated seven types of resistance patterns. The AMU and other potential sources may contribute to the presence of AMR bacteria in aquaculture. Therefore, enhanced AMU and AMR monitoring and investigation into genome characteristics are crucial to better understanding and minimizing the risk of AMR in shrimp aquaculture, particularly in Malaysia, with implications for global aquaculture practices. |
| first_indexed | 2025-11-15T14:50:24Z |
| format | Article |
| id | upm-121177 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:50:39Z |
| publishDate | 2025 |
| publisher | Elsevier B.V. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1211772025-10-29T08:06:09Z http://psasir.upm.edu.my/id/eprint/121177/ Antimicrobial resistance of zoonotic bacteria isolated from shrimp aquaculture environments in Selangor, Malaysia Devadas, S. Zakaria, Z. Shariff, M. Bhassu, S. Karim, M. Natrah, I. Intensive aquaculture farming has caused an increase in antimicrobial use (AMU) and the emergence of antimicrobial resistance (AMR). Malaysia is the ninth-largest shrimp exporter in the world. Nonetheless, the available data on AMR in shrimp aquaculture is insufficient to support efforts to combat the issue effectively. This study analyses the antimicrobial susceptibility profile and multiple antibiotic resistance (MAR) indexes of Vibrio parahaemolyticus, Escherichia coli and Salmonella in shrimp aquaculture environments in Selangor State. In total, 47 bacterial isolates, consisting of 33 (70.2 %) V. parahaemolyticus, 12 (25.5 %) E. coli and two (4.3 %) Salmonella, were isolated. Other than the intrinsically resistant-related classes of antibiotics, the V. parahaemolyticus isolates showed the top five highest percentages of resistance towards ampicillin (81.8 %, n = 27), followed by erythromycin (69.7 %, n = 23), neomycin (60.6 %, n = 20), kanamycin (54.5 %, n = 18) and ciprofloxacin (39.4 %, n = 13). Likewise, the E. coli showed resistance towards ampicillin (66.7 %, n = 8), followed by 58.3 % (n = 7) to azithromycin and cephalexin and 41.7 % (n = 5) to tetracycline, sulfamethoxazole/trimethoprim and neomycin. Thirty (63.8 %) isolates exhibited a MAR index >0.2, while 31.9 % (n = 15) were classified as multidrug-resistant (MDR) and demonstrated seven types of resistance patterns. The AMU and other potential sources may contribute to the presence of AMR bacteria in aquaculture. Therefore, enhanced AMU and AMR monitoring and investigation into genome characteristics are crucial to better understanding and minimizing the risk of AMR in shrimp aquaculture, particularly in Malaysia, with implications for global aquaculture practices. Elsevier B.V. 2025 Article NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/121177/1/121177.pdf Devadas, S. and Zakaria, Z. and Shariff, M. and Bhassu, S. and Karim, M. and Natrah, I. (2025) Antimicrobial resistance of zoonotic bacteria isolated from shrimp aquaculture environments in Selangor, Malaysia. Aquaculture, 604 (undefined). art. no. 742480. undefined-undefined. ISSN 0044-8486 https://linkinghub.elsevier.com/retrieve/pii/S0044848625003667 10.1016/j.aquaculture.2025.742480 |
| spellingShingle | Devadas, S. Zakaria, Z. Shariff, M. Bhassu, S. Karim, M. Natrah, I. Antimicrobial resistance of zoonotic bacteria isolated from shrimp aquaculture environments in Selangor, Malaysia |
| title | Antimicrobial resistance of zoonotic bacteria isolated from shrimp aquaculture environments in Selangor, Malaysia |
| title_full | Antimicrobial resistance of zoonotic bacteria isolated from shrimp aquaculture environments in Selangor, Malaysia |
| title_fullStr | Antimicrobial resistance of zoonotic bacteria isolated from shrimp aquaculture environments in Selangor, Malaysia |
| title_full_unstemmed | Antimicrobial resistance of zoonotic bacteria isolated from shrimp aquaculture environments in Selangor, Malaysia |
| title_short | Antimicrobial resistance of zoonotic bacteria isolated from shrimp aquaculture environments in Selangor, Malaysia |
| title_sort | antimicrobial resistance of zoonotic bacteria isolated from shrimp aquaculture environments in selangor, malaysia |
| url | http://psasir.upm.edu.my/id/eprint/121177/ http://psasir.upm.edu.my/id/eprint/121177/ http://psasir.upm.edu.my/id/eprint/121177/ http://psasir.upm.edu.my/id/eprint/121177/1/121177.pdf |