Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi)
Andrographis paniculata (Burm.f.) Wall. (Acanthaceae) is revered for its medicinal properties. In vitro culture of medicinal plants has assisted in improving both the quantity and quality of their yield. The current study investigated the effects of different surface sterilization treatments, plant...
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute (MDPI)
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/121110/ http://psasir.upm.edu.my/id/eprint/121110/1/121110_2023_Plant%20Growth%20Regulator-%20and%20Elicitor-Med.pdf |
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| author | Patuhai, Aicah Wahab, Puteri Edaroyati Megat Mohammad Yusoff, Martini Dewir, Yaser Hassan Alsughayyir, Ali Hakiman, Mansor |
| author_facet | Patuhai, Aicah Wahab, Puteri Edaroyati Megat Mohammad Yusoff, Martini Dewir, Yaser Hassan Alsughayyir, Ali Hakiman, Mansor |
| author_sort | Patuhai, Aicah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Andrographis paniculata (Burm.f.) Wall. (Acanthaceae) is revered for its medicinal properties. In vitro culture of medicinal plants has assisted in improving both the quantity and quality of their yield. The current study investigated the effects of different surface sterilization treatments, plant growth regulators (PGRs), and elicitors on culture establishment and axillary shoot multiplication of A. paniculata. Subsequently, the production of andrographolide in the in vitro plantlets was evaluated using high-performance liquid chromatography (HPLC) analysis. The shoot-tip explant was successfully sterilized using 60% commercial bleach for 5 min of immersion with a 90% survival rate and 96.67% aseptic culture. The optimal PGR for shoot growth was 6-benzylaminopurine (BAP) at 17.76 µM, supplemented into Murashige and Skoog (MS) media, producing 23.57 ± 0.48 leaves, 7.33 ± 0.10 shoots, and a 3.06 ± 0.02 cm length of shoots. Subsequently, MS medium supplemented with 5 mg/L chitosan produced 26.07 ± 0.14 leaves, 8.33 ± 0.07 shoots, and a 3.63 ± 0.02 cm length of shoots. The highest andrographolide content was obtained using the plantlets harvested from 5 mg/L chitosan with 2463.03 ± 0.398 µg/mL compared to the control (without elicitation) with 256.73 ± 0.341 µg/mL (859.39% increase). The results imply that the protocol for the shoot-tip culture of A. paniculata was developed, and that elicitation enhanced the herbage yield and the production of andrographolide. |
| first_indexed | 2025-11-15T14:50:06Z |
| format | Article |
| id | upm-121110 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:50:06Z |
| publishDate | 2023 |
| publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1211102025-10-27T07:02:47Z http://psasir.upm.edu.my/id/eprint/121110/ Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi) Patuhai, Aicah Wahab, Puteri Edaroyati Megat Mohammad Yusoff, Martini Dewir, Yaser Hassan Alsughayyir, Ali Hakiman, Mansor Andrographis paniculata (Burm.f.) Wall. (Acanthaceae) is revered for its medicinal properties. In vitro culture of medicinal plants has assisted in improving both the quantity and quality of their yield. The current study investigated the effects of different surface sterilization treatments, plant growth regulators (PGRs), and elicitors on culture establishment and axillary shoot multiplication of A. paniculata. Subsequently, the production of andrographolide in the in vitro plantlets was evaluated using high-performance liquid chromatography (HPLC) analysis. The shoot-tip explant was successfully sterilized using 60% commercial bleach for 5 min of immersion with a 90% survival rate and 96.67% aseptic culture. The optimal PGR for shoot growth was 6-benzylaminopurine (BAP) at 17.76 µM, supplemented into Murashige and Skoog (MS) media, producing 23.57 ± 0.48 leaves, 7.33 ± 0.10 shoots, and a 3.06 ± 0.02 cm length of shoots. Subsequently, MS medium supplemented with 5 mg/L chitosan produced 26.07 ± 0.14 leaves, 8.33 ± 0.07 shoots, and a 3.63 ± 0.02 cm length of shoots. The highest andrographolide content was obtained using the plantlets harvested from 5 mg/L chitosan with 2463.03 ± 0.398 µg/mL compared to the control (without elicitation) with 256.73 ± 0.341 µg/mL (859.39% increase). The results imply that the protocol for the shoot-tip culture of A. paniculata was developed, and that elicitation enhanced the herbage yield and the production of andrographolide. Multidisciplinary Digital Publishing Institute (MDPI) 2023 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/121110/1/121110_2023_Plant%20Growth%20Regulator-%20and%20Elicitor-Med.pdf Patuhai, Aicah and Wahab, Puteri Edaroyati Megat and Mohammad Yusoff, Martini and Dewir, Yaser Hassan and Alsughayyir, Ali and Hakiman, Mansor (2023) Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi). Plants, 12 (16). art. no. 2953. pp. 1-11. ISSN 2223-7747 https://www.mdpi.com/2223-7747/12/16/2953 10.3390/plants12162953 |
| spellingShingle | Patuhai, Aicah Wahab, Puteri Edaroyati Megat Mohammad Yusoff, Martini Dewir, Yaser Hassan Alsughayyir, Ali Hakiman, Mansor Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi) |
| title | Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi) |
| title_full | Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi) |
| title_fullStr | Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi) |
| title_full_unstemmed | Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi) |
| title_short | Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi) |
| title_sort | plant growth regulator- and elicitor-mediated enhancement of biomass and andrographolide production of shoot tip-culture-derived plantlets of andrographis paniculata (burm.f.) wall. (hempedu bumi) |
| url | http://psasir.upm.edu.my/id/eprint/121110/ http://psasir.upm.edu.my/id/eprint/121110/ http://psasir.upm.edu.my/id/eprint/121110/ http://psasir.upm.edu.my/id/eprint/121110/1/121110_2023_Plant%20Growth%20Regulator-%20and%20Elicitor-Med.pdf |