Recovery of glucose and acetic acid from Piper betle Linn leaves by subcritical water hydrolysis

Piper betle Linn (PBL) leaves contain high carbohydrates, which can be hydrolyzed into glucose and acetic acid by hydrolysis. Subcritical water hydrolysis (SWH) is an environmentally friendly method that uses water as a solvent and is suitable for hydrolysis. Thus, this study aims to evaluate the gl...

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Main Authors: Rahmah, Nur Lailatul, Mustapa Kamal, Siti Mazlina, Sulaiman, Alifdalino, Taip, Farah Saleena, Siajam, Shamsul Izhar
Format: Article
Language:English
Published: Gadjah Mada University 2025
Online Access:http://psasir.upm.edu.my/id/eprint/121075/
http://psasir.upm.edu.my/id/eprint/121075/1/121075.pdf
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author Rahmah, Nur Lailatul
Mustapa Kamal, Siti Mazlina
Sulaiman, Alifdalino
Taip, Farah Saleena
Siajam, Shamsul Izhar
author_facet Rahmah, Nur Lailatul
Mustapa Kamal, Siti Mazlina
Sulaiman, Alifdalino
Taip, Farah Saleena
Siajam, Shamsul Izhar
author_sort Rahmah, Nur Lailatul
building UPM Institutional Repository
collection Online Access
description Piper betle Linn (PBL) leaves contain high carbohydrates, which can be hydrolyzed into glucose and acetic acid by hydrolysis. Subcritical water hydrolysis (SWH) is an environmentally friendly method that uses water as a solvent and is suitable for hydrolysis. Thus, this study aims to evaluate the glucose and acetic acid recovered from PBL leaves using SWH. Using a factorial design, SWH was performed under different process conditions (temperature range 100 to 275°C and time range 5 to 30 min). Glucose and acetic acid were determined using high-performance liquid chromatography (HPLC) with a refractive index (RI) detector. The ANOVA results show that temperature, time, and the interaction of temperature and time significantly impact glucose yield and acetic acid concentration. The particle size of PBL leaves of 220.70± 2.33 µm was effective, and it can be used to recover glucose and acetic acid by SWH. The yield of glucose and acetic acid shows a different trend, with glucose degrading to acetic acid. The highest glucose concentration was achieved at 200°C for 5 min (6.143 mg/g extract), while the highest acetic acid concentration was obtained at 225°C for 20 min (2.856 mg/g extract).
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spelling upm-1210752025-10-24T04:06:44Z http://psasir.upm.edu.my/id/eprint/121075/ Recovery of glucose and acetic acid from Piper betle Linn leaves by subcritical water hydrolysis Rahmah, Nur Lailatul Mustapa Kamal, Siti Mazlina Sulaiman, Alifdalino Taip, Farah Saleena Siajam, Shamsul Izhar Piper betle Linn (PBL) leaves contain high carbohydrates, which can be hydrolyzed into glucose and acetic acid by hydrolysis. Subcritical water hydrolysis (SWH) is an environmentally friendly method that uses water as a solvent and is suitable for hydrolysis. Thus, this study aims to evaluate the glucose and acetic acid recovered from PBL leaves using SWH. Using a factorial design, SWH was performed under different process conditions (temperature range 100 to 275°C and time range 5 to 30 min). Glucose and acetic acid were determined using high-performance liquid chromatography (HPLC) with a refractive index (RI) detector. The ANOVA results show that temperature, time, and the interaction of temperature and time significantly impact glucose yield and acetic acid concentration. The particle size of PBL leaves of 220.70± 2.33 µm was effective, and it can be used to recover glucose and acetic acid by SWH. The yield of glucose and acetic acid shows a different trend, with glucose degrading to acetic acid. The highest glucose concentration was achieved at 200°C for 5 min (6.143 mg/g extract), while the highest acetic acid concentration was obtained at 225°C for 20 min (2.856 mg/g extract). Gadjah Mada University 2025-04-29 Article PeerReviewed text en cc_by_nc_4 http://psasir.upm.edu.my/id/eprint/121075/1/121075.pdf Rahmah, Nur Lailatul and Mustapa Kamal, Siti Mazlina and Sulaiman, Alifdalino and Taip, Farah Saleena and Siajam, Shamsul Izhar (2025) Recovery of glucose and acetic acid from Piper betle Linn leaves by subcritical water hydrolysis. ASEAN Journal of Chemical Engineering, 25 (1). pp. 62-74. ISSN 1655-4418; eISSN: 2655-5409 https://jurnal.ugm.ac.id/v3/AJChE/article/view/14875 10.22146/ajche.14875
spellingShingle Rahmah, Nur Lailatul
Mustapa Kamal, Siti Mazlina
Sulaiman, Alifdalino
Taip, Farah Saleena
Siajam, Shamsul Izhar
Recovery of glucose and acetic acid from Piper betle Linn leaves by subcritical water hydrolysis
title Recovery of glucose and acetic acid from Piper betle Linn leaves by subcritical water hydrolysis
title_full Recovery of glucose and acetic acid from Piper betle Linn leaves by subcritical water hydrolysis
title_fullStr Recovery of glucose and acetic acid from Piper betle Linn leaves by subcritical water hydrolysis
title_full_unstemmed Recovery of glucose and acetic acid from Piper betle Linn leaves by subcritical water hydrolysis
title_short Recovery of glucose and acetic acid from Piper betle Linn leaves by subcritical water hydrolysis
title_sort recovery of glucose and acetic acid from piper betle linn leaves by subcritical water hydrolysis
url http://psasir.upm.edu.my/id/eprint/121075/
http://psasir.upm.edu.my/id/eprint/121075/
http://psasir.upm.edu.my/id/eprint/121075/
http://psasir.upm.edu.my/id/eprint/121075/1/121075.pdf