Structural and proteomic insights into the antibiofilm efficacy of DMSO on Corynebacterium pseudotuberculosis
Corynebacterium pseudotuberculosis is an intracellular pathogen particularly responsible for causing caseous lymphadenitis in sheep and goats, resulting in substantial economic losses. Dimethyl sulfoxide (DMSO) has been shown to inhibit the formation of C. pseudotuberculosis biofilm. However, the mo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Malaya
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/121051/ http://psasir.upm.edu.my/id/eprint/121051/1/121051.pdf |
| Summary: | Corynebacterium pseudotuberculosis is an intracellular pathogen particularly responsible for causing caseous lymphadenitis in sheep and goats, resulting in substantial economic losses. Dimethyl sulfoxide (DMSO) has been shown to inhibit the formation of C. pseudotuberculosis biofilm. However, the molecular mechanisms underlying the antibiofilm efficacy of DMSO against C. pseudotuberculosis biofilm remain poorly understood. The present study was conducted to evaluate the inhibitory effects of DMSO on C. pseudotuberculosis biofilm and to determine the structural and proteomic changes in the biofilm following DMSO treatment. Pellicle assays, biomass assays, viability assays, FTIR spectroscopy, and FESEM were used to assess biofilm formation, biomass, viability, biochemical composition, and morphology of C. pseudotuberculosis, respectively. Additionally, one-dimensional SDS-PAGE in combination with tandem mass spectrometry and bioinformatics were performed to identify proteins expressed in C. pseudotuberculosis biofilm following treatment with 50% (v/v) DMSO. Results showed that DMSO treatment significantly inhibited the pellicle formation, biomass, and viability of C. pseudotuberculosis biofilm. Biochemical and morphological changes in C. pseudotuberculosis were observed following treatment with 50% (v/v) DMSO. 1D SDS-PAGE revealed differential protein expression in the range between 33.7 kDa and 150 kDa following treatment with 50% (v/v) DMSO. A total of 387 proteins were differentially expressed between the control and 50% (v/v) DMSO-treated biofilm. Analysis of the protein-protein interaction network of proteins with differential expression revealed that 50% (v/v) DMSO had an impact on 10 biological processes and 18 hub proteins. It is postulated that DMSO may inhibit C. pseudotuberculosis biofilm by modulating multiple biological pathways. |
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