Identification of gene diversity of melanocortin 4 receptor for commercial rabbit breeds
Molecular genetic markers are regularly implemented in rabbit management for better breeding by selecting for specific genotypes. This study aimed to examine the diversity of the melanocortin-4 receptor gene in rabbits in Central Java, Indonesia. A total of 35 bucks were used in this study, comprisi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Gadjah Mada University
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/121039/ http://psasir.upm.edu.my/id/eprint/121039/1/121039.pdf |
| _version_ | 1848868279532126208 |
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| author | Kamila, Firda Tasya Kamalludin, Mamat Hamidi Sutopo, Sutopo Lestari, Dela Ayu Setiatin, Enny Tantini Agustine, Ananda Dwi Latifa, Alfa Putri Nabilah, Zata Philco, Syaddad Verahry Setiaji, Asep |
| author_facet | Kamila, Firda Tasya Kamalludin, Mamat Hamidi Sutopo, Sutopo Lestari, Dela Ayu Setiatin, Enny Tantini Agustine, Ananda Dwi Latifa, Alfa Putri Nabilah, Zata Philco, Syaddad Verahry Setiaji, Asep |
| author_sort | Kamila, Firda Tasya |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Molecular genetic markers are regularly implemented in rabbit management for better breeding by selecting for specific genotypes. This study aimed to examine the diversity of the melanocortin-4 receptor gene in rabbits in Central Java, Indonesia. A total of 35 bucks were used in this study, comprising 10 New Zealand White, 11 Hyla and 14 Hycole. The DNA was extracted by Gene JET Whole Blood Genomic DNA extraction kit. Single Nucleotide Polymorphism (SNP) was detected by Polymerase Chain Reaction (PCR) and DNA sequencing. Hot carcass weight, cold carcass weight, reference weight and cut point 1-5 weight were among the parameters that were measured. This study showed that Melanocortin 4 Receptor (MC4R) genes were polymorphic, containing missense mutations and one SNP at 519G→A SNP 519GA had two alleles, A and G, with three genotypes (AA, GG, and AG). The rabbit population did not achieve Hardy-Weinberg Equilibrium (HWE) and showed a low level of genetic diversity as indicated by the heterozygosity results. The result of this study concluded that the frecuency AG as a genotype was higher than either GG or AA and A had a higher allele frequency than G. We recommended improving the validity of the data across various rabbit populations. |
| first_indexed | 2025-11-15T14:49:52Z |
| format | Article |
| id | upm-121039 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:49:52Z |
| publishDate | 2025 |
| publisher | Gadjah Mada University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1210392025-10-23T01:32:38Z http://psasir.upm.edu.my/id/eprint/121039/ Identification of gene diversity of melanocortin 4 receptor for commercial rabbit breeds Kamila, Firda Tasya Kamalludin, Mamat Hamidi Sutopo, Sutopo Lestari, Dela Ayu Setiatin, Enny Tantini Agustine, Ananda Dwi Latifa, Alfa Putri Nabilah, Zata Philco, Syaddad Verahry Setiaji, Asep Molecular genetic markers are regularly implemented in rabbit management for better breeding by selecting for specific genotypes. This study aimed to examine the diversity of the melanocortin-4 receptor gene in rabbits in Central Java, Indonesia. A total of 35 bucks were used in this study, comprising 10 New Zealand White, 11 Hyla and 14 Hycole. The DNA was extracted by Gene JET Whole Blood Genomic DNA extraction kit. Single Nucleotide Polymorphism (SNP) was detected by Polymerase Chain Reaction (PCR) and DNA sequencing. Hot carcass weight, cold carcass weight, reference weight and cut point 1-5 weight were among the parameters that were measured. This study showed that Melanocortin 4 Receptor (MC4R) genes were polymorphic, containing missense mutations and one SNP at 519G→A SNP 519GA had two alleles, A and G, with three genotypes (AA, GG, and AG). The rabbit population did not achieve Hardy-Weinberg Equilibrium (HWE) and showed a low level of genetic diversity as indicated by the heterozygosity results. The result of this study concluded that the frecuency AG as a genotype was higher than either GG or AA and A had a higher allele frequency than G. We recommended improving the validity of the data across various rabbit populations. Gadjah Mada University 2025 Article PeerReviewed text en cc_by_sa_4 http://psasir.upm.edu.my/id/eprint/121039/1/121039.pdf Kamila, Firda Tasya and Kamalludin, Mamat Hamidi and Sutopo, Sutopo and Lestari, Dela Ayu and Setiatin, Enny Tantini and Agustine, Ananda Dwi and Latifa, Alfa Putri and Nabilah, Zata and Philco, Syaddad Verahry and Setiaji, Asep (2025) Identification of gene diversity of melanocortin 4 receptor for commercial rabbit breeds. Buletin Peternakan, 49 (1). pp. 28-34. ISSN 0126-4400; eISSN: 2407-876X https://jurnal.ugm.ac.id/buletinpeternakan/article/view/99303 10.21059/buletinpeternak.v49i1.99303 |
| spellingShingle | Kamila, Firda Tasya Kamalludin, Mamat Hamidi Sutopo, Sutopo Lestari, Dela Ayu Setiatin, Enny Tantini Agustine, Ananda Dwi Latifa, Alfa Putri Nabilah, Zata Philco, Syaddad Verahry Setiaji, Asep Identification of gene diversity of melanocortin 4 receptor for commercial rabbit breeds |
| title | Identification of gene diversity of melanocortin 4 receptor for commercial rabbit breeds |
| title_full | Identification of gene diversity of melanocortin 4 receptor for commercial rabbit breeds |
| title_fullStr | Identification of gene diversity of melanocortin 4 receptor for commercial rabbit breeds |
| title_full_unstemmed | Identification of gene diversity of melanocortin 4 receptor for commercial rabbit breeds |
| title_short | Identification of gene diversity of melanocortin 4 receptor for commercial rabbit breeds |
| title_sort | identification of gene diversity of melanocortin 4 receptor for commercial rabbit breeds |
| url | http://psasir.upm.edu.my/id/eprint/121039/ http://psasir.upm.edu.my/id/eprint/121039/ http://psasir.upm.edu.my/id/eprint/121039/ http://psasir.upm.edu.my/id/eprint/121039/1/121039.pdf |