Jasmonic acid signaling pathway is involved in agaro-oligosaccharides-induced disease resistance of peach fruit through PpMYC2-activated transcription of PpPGIP1
Agaro-oligosaccharides (AO) can enhance peach fruit resistance to Monilinia fructicola and reduce postharvest decay, but its molecular mechanism is unclear. In this study, transcriptome analysis and RT-qPCR analysis revealed that AO activated the expression of genes associated with jasmonic acid (JA...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier B.V.
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/121020/ http://psasir.upm.edu.my/id/eprint/121020/1/121020.pdf |
| _version_ | 1848868274709725184 |
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| author | Li, Qian Wei, Yingying Jiang, Shu Ye, Jianfen Chen, Yi Xu, Feng Lou, Yongjiang Ding, Phebe Shao, Xingfeng |
| author_facet | Li, Qian Wei, Yingying Jiang, Shu Ye, Jianfen Chen, Yi Xu, Feng Lou, Yongjiang Ding, Phebe Shao, Xingfeng |
| author_sort | Li, Qian |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Agaro-oligosaccharides (AO) can enhance peach fruit resistance to Monilinia fructicola and reduce postharvest decay, but its molecular mechanism is unclear. In this study, transcriptome analysis and RT-qPCR analysis revealed that AO activated the expression of genes associated with jasmonic acid (JA) synthesis (PpLOX5–1/PpLOX5–2/PpAOC/PpAOS/PpOPR2) and signal transduction (PpMYC2/PpJAZ5), along with a rise in JA content. Additionally, AO significantly up-regulated the expression of the gene encoding polygalacturonase-inhibiting protein 1 (PpPGIP1) participating in plant-pathogen interaction. Yeast one-hybrid assay and electrophoretic mobility shift assay revealed that PpMYC2 bound to the PpPGIP1 promoter through the G-box element. Dual luciferase reporter assay in tobacco, as well as transient overexpression and silencing of PpMYC2 in peach fruit, demonstrated that PpMYC2 positively regulated PpPGIP1 expression. In summary, our findings indicated that AO activated JA synthesis and signal transduction, subsequently triggering PpMYC2 to positively regulate PpPGIP1 expression, thereby boosting the resistance of peach fruit to M. fructicola infection. |
| first_indexed | 2025-11-15T14:49:47Z |
| format | Article |
| id | upm-121020 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:49:47Z |
| publishDate | 2025 |
| publisher | Elsevier B.V. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1210202025-10-22T02:57:11Z http://psasir.upm.edu.my/id/eprint/121020/ Jasmonic acid signaling pathway is involved in agaro-oligosaccharides-induced disease resistance of peach fruit through PpMYC2-activated transcription of PpPGIP1 Li, Qian Wei, Yingying Jiang, Shu Ye, Jianfen Chen, Yi Xu, Feng Lou, Yongjiang Ding, Phebe Shao, Xingfeng Agaro-oligosaccharides (AO) can enhance peach fruit resistance to Monilinia fructicola and reduce postharvest decay, but its molecular mechanism is unclear. In this study, transcriptome analysis and RT-qPCR analysis revealed that AO activated the expression of genes associated with jasmonic acid (JA) synthesis (PpLOX5–1/PpLOX5–2/PpAOC/PpAOS/PpOPR2) and signal transduction (PpMYC2/PpJAZ5), along with a rise in JA content. Additionally, AO significantly up-regulated the expression of the gene encoding polygalacturonase-inhibiting protein 1 (PpPGIP1) participating in plant-pathogen interaction. Yeast one-hybrid assay and electrophoretic mobility shift assay revealed that PpMYC2 bound to the PpPGIP1 promoter through the G-box element. Dual luciferase reporter assay in tobacco, as well as transient overexpression and silencing of PpMYC2 in peach fruit, demonstrated that PpMYC2 positively regulated PpPGIP1 expression. In summary, our findings indicated that AO activated JA synthesis and signal transduction, subsequently triggering PpMYC2 to positively regulate PpPGIP1 expression, thereby boosting the resistance of peach fruit to M. fructicola infection. Elsevier B.V. 2025-02-24 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/121020/1/121020.pdf Li, Qian and Wei, Yingying and Jiang, Shu and Ye, Jianfen and Chen, Yi and Xu, Feng and Lou, Yongjiang and Ding, Phebe and Shao, Xingfeng (2025) Jasmonic acid signaling pathway is involved in agaro-oligosaccharides-induced disease resistance of peach fruit through PpMYC2-activated transcription of PpPGIP1. Postharvest Biology and Technology, 224. art. no. 113477. pp. 1-10. ISSN 0925-5214 https://linkinghub.elsevier.com/retrieve/pii/S0925521425000894 10.1016/j.postharvbio.2025.113477 |
| spellingShingle | Li, Qian Wei, Yingying Jiang, Shu Ye, Jianfen Chen, Yi Xu, Feng Lou, Yongjiang Ding, Phebe Shao, Xingfeng Jasmonic acid signaling pathway is involved in agaro-oligosaccharides-induced disease resistance of peach fruit through PpMYC2-activated transcription of PpPGIP1 |
| title | Jasmonic acid signaling pathway is involved in agaro-oligosaccharides-induced disease resistance of peach fruit through PpMYC2-activated transcription of PpPGIP1 |
| title_full | Jasmonic acid signaling pathway is involved in agaro-oligosaccharides-induced disease resistance of peach fruit through PpMYC2-activated transcription of PpPGIP1 |
| title_fullStr | Jasmonic acid signaling pathway is involved in agaro-oligosaccharides-induced disease resistance of peach fruit through PpMYC2-activated transcription of PpPGIP1 |
| title_full_unstemmed | Jasmonic acid signaling pathway is involved in agaro-oligosaccharides-induced disease resistance of peach fruit through PpMYC2-activated transcription of PpPGIP1 |
| title_short | Jasmonic acid signaling pathway is involved in agaro-oligosaccharides-induced disease resistance of peach fruit through PpMYC2-activated transcription of PpPGIP1 |
| title_sort | jasmonic acid signaling pathway is involved in agaro-oligosaccharides-induced disease resistance of peach fruit through ppmyc2-activated transcription of pppgip1 |
| url | http://psasir.upm.edu.my/id/eprint/121020/ http://psasir.upm.edu.my/id/eprint/121020/ http://psasir.upm.edu.my/id/eprint/121020/ http://psasir.upm.edu.my/id/eprint/121020/1/121020.pdf |