Cardio- and neuroprotective effects by pretreatment of dietary moringin from Moringa oleifera seeds and α-CD/moringin formulation in a rat model of isoproterenol-induced myocardial infarction
The aim of this study was to investigated the cardio- and neuroprotective effects of moringin (MG), a dietary isothiocyanate derived from Moringa oleifera seeds, in a rat model of isoproterenol (ISP)-induced myocardial infarction (MI). Thirty-two adult male Sprague Dawley rats were divided into four...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120969/ http://psasir.upm.edu.my/id/eprint/120969/1/120969.pdf |
| _version_ | 1848868261557436416 |
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| author | Razis, Ahmad Faizal Abdull Kamal, Ramla Muhammad De Nicola, Gina Rosalinda Montaut, Sabine Perimal, Enoch Kumar Ahmad, Hafandi Rollin, Patrick Rigaud, Sébastien Mazzon, Emanuela Djedaini-Pilard, Florence |
| author_facet | Razis, Ahmad Faizal Abdull Kamal, Ramla Muhammad De Nicola, Gina Rosalinda Montaut, Sabine Perimal, Enoch Kumar Ahmad, Hafandi Rollin, Patrick Rigaud, Sébastien Mazzon, Emanuela Djedaini-Pilard, Florence |
| author_sort | Razis, Ahmad Faizal Abdull |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The aim of this study was to investigated the cardio- and neuroprotective effects of moringin (MG), a dietary isothiocyanate derived from Moringa oleifera seeds, in a rat model of isoproterenol (ISP)-induced myocardial infarction (MI). Thirty-two adult male Sprague Dawley rats were divided into four groups: a control group, an MI group, a group pretreated with freshly prepared MG solution (MG + MI; glucomoringin 20 mg/kg + 30 µl myrosinase/rat), and a group pretreated with a stable α-cyclodextrin-based formulation of MG (α-CD/MG + MI, 42 mg/kg). Pretreatments were administered daily for seven days, followed by ISP injections (85 mg/kg, subcutaneously) on days six and seven at 24-hour intervals to induce MI. The MI group showed impaired hemodynamic and behavioral responses, elevated malondialdehyde (MDA) levels, and decreased activity of antioxidant enzymes—superoxide dismutase (SOD) and catalase (CAT)—in both myocardial and hippocampal tissues. In addition, serum levels of cardiac biomarkers, including cardiac troponin I (cTnI) and creatine kinase myocardial band (CK-MB), were significantly increased. Pretreatment with MG and α-CD/MG markedly improved locomotor and exploratory behavior, reduced heart rate (HR), and enhanced mean arterial pressure (MAP). Both treatments also decreased serum cardiac marker levels, restored antioxidant enzyme activity, normalized brain monoamine concentrations, and improved the structural integrity of myocardial and hippocampal tissues. These findings indicate that MG and α-CD/MG confer cardioprotective and neuroprotective benefits by attenuating oxidative stress and maintaining redox balance in ISP-induced MI, suggesting their potential as effective pretreatment strategies to mitigate the systemic complications associated with myocardial infarction. |
| first_indexed | 2025-11-15T14:49:35Z |
| format | Article |
| id | upm-120969 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:49:35Z |
| publishDate | 2025 |
| publisher | Cambridge University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1209692025-10-17T03:50:58Z http://psasir.upm.edu.my/id/eprint/120969/ Cardio- and neuroprotective effects by pretreatment of dietary moringin from Moringa oleifera seeds and α-CD/moringin formulation in a rat model of isoproterenol-induced myocardial infarction Razis, Ahmad Faizal Abdull Kamal, Ramla Muhammad De Nicola, Gina Rosalinda Montaut, Sabine Perimal, Enoch Kumar Ahmad, Hafandi Rollin, Patrick Rigaud, Sébastien Mazzon, Emanuela Djedaini-Pilard, Florence The aim of this study was to investigated the cardio- and neuroprotective effects of moringin (MG), a dietary isothiocyanate derived from Moringa oleifera seeds, in a rat model of isoproterenol (ISP)-induced myocardial infarction (MI). Thirty-two adult male Sprague Dawley rats were divided into four groups: a control group, an MI group, a group pretreated with freshly prepared MG solution (MG + MI; glucomoringin 20 mg/kg + 30 µl myrosinase/rat), and a group pretreated with a stable α-cyclodextrin-based formulation of MG (α-CD/MG + MI, 42 mg/kg). Pretreatments were administered daily for seven days, followed by ISP injections (85 mg/kg, subcutaneously) on days six and seven at 24-hour intervals to induce MI. The MI group showed impaired hemodynamic and behavioral responses, elevated malondialdehyde (MDA) levels, and decreased activity of antioxidant enzymes—superoxide dismutase (SOD) and catalase (CAT)—in both myocardial and hippocampal tissues. In addition, serum levels of cardiac biomarkers, including cardiac troponin I (cTnI) and creatine kinase myocardial band (CK-MB), were significantly increased. Pretreatment with MG and α-CD/MG markedly improved locomotor and exploratory behavior, reduced heart rate (HR), and enhanced mean arterial pressure (MAP). Both treatments also decreased serum cardiac marker levels, restored antioxidant enzyme activity, normalized brain monoamine concentrations, and improved the structural integrity of myocardial and hippocampal tissues. These findings indicate that MG and α-CD/MG confer cardioprotective and neuroprotective benefits by attenuating oxidative stress and maintaining redox balance in ISP-induced MI, suggesting their potential as effective pretreatment strategies to mitigate the systemic complications associated with myocardial infarction. Cambridge University Press 2025-09-04 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/120969/1/120969.pdf Razis, Ahmad Faizal Abdull and Kamal, Ramla Muhammad and De Nicola, Gina Rosalinda and Montaut, Sabine and Perimal, Enoch Kumar and Ahmad, Hafandi and Rollin, Patrick and Rigaud, Sébastien and Mazzon, Emanuela and Djedaini-Pilard, Florence (2025) Cardio- and neuroprotective effects by pretreatment of dietary moringin from Moringa oleifera seeds and α-CD/moringin formulation in a rat model of isoproterenol-induced myocardial infarction. Journal of Nutritional Science, 14. art. no. e62. pp. 1-13. ISSN 2048-6790 https://doi.org/10.1017/jns.2025.10035 10.1017/jns.2025.10035 |
| spellingShingle | Razis, Ahmad Faizal Abdull Kamal, Ramla Muhammad De Nicola, Gina Rosalinda Montaut, Sabine Perimal, Enoch Kumar Ahmad, Hafandi Rollin, Patrick Rigaud, Sébastien Mazzon, Emanuela Djedaini-Pilard, Florence Cardio- and neuroprotective effects by pretreatment of dietary moringin from Moringa oleifera seeds and α-CD/moringin formulation in a rat model of isoproterenol-induced myocardial infarction |
| title | Cardio- and neuroprotective effects by pretreatment of dietary moringin from Moringa oleifera seeds and α-CD/moringin formulation in a rat model of isoproterenol-induced myocardial infarction |
| title_full | Cardio- and neuroprotective effects by pretreatment of dietary moringin from Moringa oleifera seeds and α-CD/moringin formulation in a rat model of isoproterenol-induced myocardial infarction |
| title_fullStr | Cardio- and neuroprotective effects by pretreatment of dietary moringin from Moringa oleifera seeds and α-CD/moringin formulation in a rat model of isoproterenol-induced myocardial infarction |
| title_full_unstemmed | Cardio- and neuroprotective effects by pretreatment of dietary moringin from Moringa oleifera seeds and α-CD/moringin formulation in a rat model of isoproterenol-induced myocardial infarction |
| title_short | Cardio- and neuroprotective effects by pretreatment of dietary moringin from Moringa oleifera seeds and α-CD/moringin formulation in a rat model of isoproterenol-induced myocardial infarction |
| title_sort | cardio- and neuroprotective effects by pretreatment of dietary moringin from moringa oleifera seeds and α-cd/moringin formulation in a rat model of isoproterenol-induced myocardial infarction |
| url | http://psasir.upm.edu.my/id/eprint/120969/ http://psasir.upm.edu.my/id/eprint/120969/ http://psasir.upm.edu.my/id/eprint/120969/ http://psasir.upm.edu.my/id/eprint/120969/1/120969.pdf |