Comparative genotyping of Malaysian clinical isolates of Streptococcus pneumoniae by multilocus sequence typing and multilocus variable-number tandem repeat analysis
Background: Streptococcus pneumoniae (pneumococcus) is an opportunistic pathogen that causes severe upper and lower respiratory tract infections, leading to life-threatening diseases. This study aims to determine the genetic variation of serotypes among a collection of clinical S. pneumoniae isolate...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Sains Malaysia
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120945/ http://psasir.upm.edu.my/id/eprint/120945/1/120945.pdf |
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| author | Abdul Rahman, Nurul Asyikin Muthanna, Abdulrahman Mohd Desa, Mohd Nasir Dzaraly, Nurul Diana Hazman, Hazmin Sulaiman, Nurshahira Mohamad, Norfazlina Meor Yahaya, Maitasha Alia Al-Obaidi, Mazen M Jamil Mohd Taib, Niazlin Masri, Siti Norbaya Al-Obaidi, Mazen M Jamil |
| author_facet | Abdul Rahman, Nurul Asyikin Muthanna, Abdulrahman Mohd Desa, Mohd Nasir Dzaraly, Nurul Diana Hazman, Hazmin Sulaiman, Nurshahira Mohamad, Norfazlina Meor Yahaya, Maitasha Alia Al-Obaidi, Mazen M Jamil Mohd Taib, Niazlin Masri, Siti Norbaya Al-Obaidi, Mazen M Jamil |
| author_sort | Abdul Rahman, Nurul Asyikin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Streptococcus pneumoniae (pneumococcus) is an opportunistic pathogen that causes severe upper and lower respiratory tract infections, leading to life-threatening diseases. This study aims to determine the genetic variation of serotypes among a collection of clinical S. pneumoniae isolates using multilocus sequence typing (MLST) and multilocus variable-number tandem repeat analysis (MLVA). Method: A total of 103 viable isolates were serotyped and subjected to MLVA; only those with discrete serotypes (n = 91) were subjected to MLST analysis. Results: The comparative phylogenetic analysis resulted in the segregation of clonal complexes (CC) and 36 singletons accordingly. The major clonal complex, MLST CC320 (n = 23; 25.3%), had a close association with the Taiwan 19F-14 clone, consisting of ST236, ST271, and ST320. The second largest group, MLST CC9 (n = 12; 13.2%), had an association with the England14-9 clone, comprising ST7 and ST9. MLVA analysis demonstrated its ability to differentiate subgroups within CCs that share the same sequence type (ST) with distinct MLVA types (MTs). Conclusion: The integration of MLST and MLVA in this study serves as a model for pneumococcal surveillance indicating some well-known globally circulating clones that have been persisting at this study setting. |
| first_indexed | 2025-11-15T14:49:28Z |
| format | Article |
| id | upm-120945 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:49:28Z |
| publishDate | 2025 |
| publisher | Penerbit Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1209452025-10-16T07:05:12Z http://psasir.upm.edu.my/id/eprint/120945/ Comparative genotyping of Malaysian clinical isolates of Streptococcus pneumoniae by multilocus sequence typing and multilocus variable-number tandem repeat analysis Abdul Rahman, Nurul Asyikin Muthanna, Abdulrahman Mohd Desa, Mohd Nasir Dzaraly, Nurul Diana Hazman, Hazmin Sulaiman, Nurshahira Mohamad, Norfazlina Meor Yahaya, Maitasha Alia Al-Obaidi, Mazen M Jamil Mohd Taib, Niazlin Masri, Siti Norbaya Al-Obaidi, Mazen M Jamil Background: Streptococcus pneumoniae (pneumococcus) is an opportunistic pathogen that causes severe upper and lower respiratory tract infections, leading to life-threatening diseases. This study aims to determine the genetic variation of serotypes among a collection of clinical S. pneumoniae isolates using multilocus sequence typing (MLST) and multilocus variable-number tandem repeat analysis (MLVA). Method: A total of 103 viable isolates were serotyped and subjected to MLVA; only those with discrete serotypes (n = 91) were subjected to MLST analysis. Results: The comparative phylogenetic analysis resulted in the segregation of clonal complexes (CC) and 36 singletons accordingly. The major clonal complex, MLST CC320 (n = 23; 25.3%), had a close association with the Taiwan 19F-14 clone, consisting of ST236, ST271, and ST320. The second largest group, MLST CC9 (n = 12; 13.2%), had an association with the England14-9 clone, comprising ST7 and ST9. MLVA analysis demonstrated its ability to differentiate subgroups within CCs that share the same sequence type (ST) with distinct MLVA types (MTs). Conclusion: The integration of MLST and MLVA in this study serves as a model for pneumococcal surveillance indicating some well-known globally circulating clones that have been persisting at this study setting. Penerbit Universiti Sains Malaysia 2025 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/120945/1/120945.pdf Abdul Rahman, Nurul Asyikin and Muthanna, Abdulrahman and Mohd Desa, Mohd Nasir and Dzaraly, Nurul Diana and Hazman, Hazmin and Sulaiman, Nurshahira and Mohamad, Norfazlina and Meor Yahaya, Maitasha Alia and Al-Obaidi, Mazen M Jamil and Mohd Taib, Niazlin and Masri, Siti Norbaya and Al-Obaidi, Mazen M Jamil (2025) Comparative genotyping of Malaysian clinical isolates of Streptococcus pneumoniae by multilocus sequence typing and multilocus variable-number tandem repeat analysis. Malaysian Journal of Medical Sciences, 32 (1). pp. 69-87. ISSN 1394-195X; eISSN: 2180-4303 http://www.mjms.usm.my/MJMS32012025/07_mjms-09-2024-677.pdf 10.21315/mjms-09-2024-677 |
| spellingShingle | Abdul Rahman, Nurul Asyikin Muthanna, Abdulrahman Mohd Desa, Mohd Nasir Dzaraly, Nurul Diana Hazman, Hazmin Sulaiman, Nurshahira Mohamad, Norfazlina Meor Yahaya, Maitasha Alia Al-Obaidi, Mazen M Jamil Mohd Taib, Niazlin Masri, Siti Norbaya Al-Obaidi, Mazen M Jamil Comparative genotyping of Malaysian clinical isolates of Streptococcus pneumoniae by multilocus sequence typing and multilocus variable-number tandem repeat analysis |
| title | Comparative genotyping of Malaysian clinical isolates of Streptococcus pneumoniae by multilocus sequence typing and multilocus variable-number tandem repeat analysis |
| title_full | Comparative genotyping of Malaysian clinical isolates of Streptococcus pneumoniae by multilocus sequence typing and multilocus variable-number tandem repeat analysis |
| title_fullStr | Comparative genotyping of Malaysian clinical isolates of Streptococcus pneumoniae by multilocus sequence typing and multilocus variable-number tandem repeat analysis |
| title_full_unstemmed | Comparative genotyping of Malaysian clinical isolates of Streptococcus pneumoniae by multilocus sequence typing and multilocus variable-number tandem repeat analysis |
| title_short | Comparative genotyping of Malaysian clinical isolates of Streptococcus pneumoniae by multilocus sequence typing and multilocus variable-number tandem repeat analysis |
| title_sort | comparative genotyping of malaysian clinical isolates of streptococcus pneumoniae by multilocus sequence typing and multilocus variable-number tandem repeat analysis |
| url | http://psasir.upm.edu.my/id/eprint/120945/ http://psasir.upm.edu.my/id/eprint/120945/ http://psasir.upm.edu.my/id/eprint/120945/ http://psasir.upm.edu.my/id/eprint/120945/1/120945.pdf |