In-silico and phylogenetic analysis of acetate: succinate coatransferase (ASCT) from Angiostrongylus malaysiensis
The zoonotic capability of Angiostrongylus malaysiensis was recently observed after several years of doubt. This parasite was found in a high burden of Malaysian rats, which is alarming. There is currently no effective treatment for human neuroangiostrongyliasis. Acetate: succinate CoA-transferase (...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120902/ http://psasir.upm.edu.my/id/eprint/120902/1/120902.pdf |
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| author | Sipin, Quincie Suey, Yee Low Wan Ahmad Kamil, Wan Nur Ismah Wan, Kiew-Lian Ajat, Mokrish Kamaludeen, Juriah Syed-Hussain, Sharifah Salmah Ahmad, Nur Indah Abdul Aziz, Nor Azlina |
| author_facet | Sipin, Quincie Suey, Yee Low Wan Ahmad Kamil, Wan Nur Ismah Wan, Kiew-Lian Ajat, Mokrish Kamaludeen, Juriah Syed-Hussain, Sharifah Salmah Ahmad, Nur Indah Abdul Aziz, Nor Azlina |
| author_sort | Sipin, Quincie |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The zoonotic capability of Angiostrongylus malaysiensis was recently observed after several years of doubt. This parasite was found in a high burden of Malaysian rats, which is alarming. There is currently no effective treatment for human neuroangiostrongyliasis. Acetate: succinate CoA-transferase (ASCT) enzyme catalyses acetate production in helminth parasites. ASCT was classified into three subfamilies within the family I CoA-transferases (IA, IB, and IC). Acetate is an essential metabolic end product of many parasites, making it an attractive drug target since it is absent in mammalian hosts. The current study describes the in-silico analysis conducted for the identification and phylogenetic characterisation of A. malaysiensis ASCT and genetic variations between subfamilies of ASCT. The AmASCT was identified from the ongoing de novo transcriptome assembly and annotation of adult A. malaysiensis. The analysis of AmASCT physiochemical properties, multiple sequence alignment and phylogenetic relations with the ASCTs of other helminths are conducted using standard bioinformatic tools. Pairwise comparisons between subfamilies of ASCT have also been conducted in silico. AmASCT has the conserved regions of the family I CoA-transferases and is clustered with subfamily IB of ASCT. From the pairwise analysis, subfamilies IB and IC were most closely related between the three subfamilies. AmASCT was predicted to be overall hydrophilic and stable in a neutral to slightly alkaline environment within the parasite. The phylogenetic analysis confirmed that AmASCT belongs to subfamily IB of ASCTs. Further study on the biochemical activity of ASCT in A. malaysiensis is required to determine its enzymatic function. |
| first_indexed | 2025-11-15T14:49:16Z |
| format | Article |
| id | upm-120902 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:49:16Z |
| publishDate | 2025 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1209022025-10-15T03:22:39Z http://psasir.upm.edu.my/id/eprint/120902/ In-silico and phylogenetic analysis of acetate: succinate coatransferase (ASCT) from Angiostrongylus malaysiensis Sipin, Quincie Suey, Yee Low Wan Ahmad Kamil, Wan Nur Ismah Wan, Kiew-Lian Ajat, Mokrish Kamaludeen, Juriah Syed-Hussain, Sharifah Salmah Ahmad, Nur Indah Abdul Aziz, Nor Azlina The zoonotic capability of Angiostrongylus malaysiensis was recently observed after several years of doubt. This parasite was found in a high burden of Malaysian rats, which is alarming. There is currently no effective treatment for human neuroangiostrongyliasis. Acetate: succinate CoA-transferase (ASCT) enzyme catalyses acetate production in helminth parasites. ASCT was classified into three subfamilies within the family I CoA-transferases (IA, IB, and IC). Acetate is an essential metabolic end product of many parasites, making it an attractive drug target since it is absent in mammalian hosts. The current study describes the in-silico analysis conducted for the identification and phylogenetic characterisation of A. malaysiensis ASCT and genetic variations between subfamilies of ASCT. The AmASCT was identified from the ongoing de novo transcriptome assembly and annotation of adult A. malaysiensis. The analysis of AmASCT physiochemical properties, multiple sequence alignment and phylogenetic relations with the ASCTs of other helminths are conducted using standard bioinformatic tools. Pairwise comparisons between subfamilies of ASCT have also been conducted in silico. AmASCT has the conserved regions of the family I CoA-transferases and is clustered with subfamily IB of ASCT. From the pairwise analysis, subfamilies IB and IC were most closely related between the three subfamilies. AmASCT was predicted to be overall hydrophilic and stable in a neutral to slightly alkaline environment within the parasite. The phylogenetic analysis confirmed that AmASCT belongs to subfamily IB of ASCTs. Further study on the biochemical activity of ASCT in A. malaysiensis is required to determine its enzymatic function. Universiti Putra Malaysia 2025 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/120902/1/120902.pdf Sipin, Quincie and Suey, Yee Low and Wan Ahmad Kamil, Wan Nur Ismah and Wan, Kiew-Lian and Ajat, Mokrish and Kamaludeen, Juriah and Syed-Hussain, Sharifah Salmah and Ahmad, Nur Indah and Abdul Aziz, Nor Azlina (2025) In-silico and phylogenetic analysis of acetate: succinate coatransferase (ASCT) from Angiostrongylus malaysiensis. Pertanika Journal of Tropical Agricultural Science, 48 (3). pp. 747-766. ISSN 1511-3701; eISSN: 2231-8542 http://pertanika2.upm.edu.my/pjtas/browse/regular-issue?article=JTAS-3175-2024 10.47836/pjtas.48.3.06 |
| spellingShingle | Sipin, Quincie Suey, Yee Low Wan Ahmad Kamil, Wan Nur Ismah Wan, Kiew-Lian Ajat, Mokrish Kamaludeen, Juriah Syed-Hussain, Sharifah Salmah Ahmad, Nur Indah Abdul Aziz, Nor Azlina In-silico and phylogenetic analysis of acetate: succinate coatransferase (ASCT) from Angiostrongylus malaysiensis |
| title | In-silico and phylogenetic analysis of acetate: succinate coatransferase (ASCT) from Angiostrongylus malaysiensis |
| title_full | In-silico and phylogenetic analysis of acetate: succinate coatransferase (ASCT) from Angiostrongylus malaysiensis |
| title_fullStr | In-silico and phylogenetic analysis of acetate: succinate coatransferase (ASCT) from Angiostrongylus malaysiensis |
| title_full_unstemmed | In-silico and phylogenetic analysis of acetate: succinate coatransferase (ASCT) from Angiostrongylus malaysiensis |
| title_short | In-silico and phylogenetic analysis of acetate: succinate coatransferase (ASCT) from Angiostrongylus malaysiensis |
| title_sort | in-silico and phylogenetic analysis of acetate: succinate coatransferase (asct) from angiostrongylus malaysiensis |
| url | http://psasir.upm.edu.my/id/eprint/120902/ http://psasir.upm.edu.my/id/eprint/120902/ http://psasir.upm.edu.my/id/eprint/120902/ http://psasir.upm.edu.my/id/eprint/120902/1/120902.pdf |