Effect of N, N-dimethylglycine (DMG) supplementation on haematological parameters and frequency of CD4+ and CD8+ T cells in cats post-vaccination
N, N-Dimethylglycine (DMG) is a commonly used nutraceutical in veterinary medicine, which is claimed to have immunomodulating properties. This novel study investigated the effect of DMG supplementation on feline haematological parameters and the percentages of CD4+ and CD8+ T cells after vaccination...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120899/ http://psasir.upm.edu.my/id/eprint/120899/1/120899.pdf |
| _version_ | 1848868241161584640 |
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| author | Shahril Agus, Syahir Aiman Shamsul-Bahri, Nurul Afiqah Juliana Syafinaz, . Mohd Sadali, Muhammad Farris Hazilawati Hamzah, . Mustaffa-Kamal, Farina |
| author_facet | Shahril Agus, Syahir Aiman Shamsul-Bahri, Nurul Afiqah Juliana Syafinaz, . Mohd Sadali, Muhammad Farris Hazilawati Hamzah, . Mustaffa-Kamal, Farina |
| author_sort | Shahril Agus, Syahir Aiman |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | N, N-Dimethylglycine (DMG) is a commonly used nutraceutical in veterinary medicine, which is claimed to have immunomodulating properties. This novel study investigated the effect of DMG supplementation on feline haematological parameters and the percentages of CD4+ and CD8+ T cells after vaccination with a single-dose commercial core vaccine. A total of twelve neutered cats were divided into control (n=6) and treatment groups (n=6) and received one dose of feline core vaccine at day 0. The treatment group then received oral DMG supplementation (125 mg/mL) twice daily for 14 days. Blood samples were collected on days 0 and 15 for haematological, differential cell count, and T cell phenotyping analysis. Haematological analysis revealed no significant difference between the control and treatment groups after 14 days of the experiment. At post-treatment, the neutrophil percentage of the treatment group was significantly lower (p=0.0238) compared to the control group, while the lymphocyte percentage of the treatment group was significantly higher (p=0.013) compared to the control group. The reduction in neutrophil percentage could be due to bromelain, an anti-inflammatory enzyme, while an increase in lymphocyte percentage could be attributed to an increase in B cell or NK cell subsets instead of T cells. Future research is warranted to investigate the effects of DMG on B cells and natural killer cell activation and to explore the long-term effects of DMG supplementation on feline immune health. |
| first_indexed | 2025-11-15T14:49:15Z |
| format | Article |
| id | upm-120899 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:49:15Z |
| publishDate | 2025 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1208992025-10-15T03:10:07Z http://psasir.upm.edu.my/id/eprint/120899/ Effect of N, N-dimethylglycine (DMG) supplementation on haematological parameters and frequency of CD4+ and CD8+ T cells in cats post-vaccination Shahril Agus, Syahir Aiman Shamsul-Bahri, Nurul Afiqah Juliana Syafinaz, . Mohd Sadali, Muhammad Farris Hazilawati Hamzah, . Mustaffa-Kamal, Farina N, N-Dimethylglycine (DMG) is a commonly used nutraceutical in veterinary medicine, which is claimed to have immunomodulating properties. This novel study investigated the effect of DMG supplementation on feline haematological parameters and the percentages of CD4+ and CD8+ T cells after vaccination with a single-dose commercial core vaccine. A total of twelve neutered cats were divided into control (n=6) and treatment groups (n=6) and received one dose of feline core vaccine at day 0. The treatment group then received oral DMG supplementation (125 mg/mL) twice daily for 14 days. Blood samples were collected on days 0 and 15 for haematological, differential cell count, and T cell phenotyping analysis. Haematological analysis revealed no significant difference between the control and treatment groups after 14 days of the experiment. At post-treatment, the neutrophil percentage of the treatment group was significantly lower (p=0.0238) compared to the control group, while the lymphocyte percentage of the treatment group was significantly higher (p=0.013) compared to the control group. The reduction in neutrophil percentage could be due to bromelain, an anti-inflammatory enzyme, while an increase in lymphocyte percentage could be attributed to an increase in B cell or NK cell subsets instead of T cells. Future research is warranted to investigate the effects of DMG on B cells and natural killer cell activation and to explore the long-term effects of DMG supplementation on feline immune health. Universiti Putra Malaysia 2025 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/120899/1/120899.pdf Shahril Agus, Syahir Aiman and Shamsul-Bahri, Nurul Afiqah and Juliana Syafinaz, . and Mohd Sadali, Muhammad Farris and Hazilawati Hamzah, . and Mustaffa-Kamal, Farina (2025) Effect of N, N-dimethylglycine (DMG) supplementation on haematological parameters and frequency of CD4+ and CD8+ T cells in cats post-vaccination. Pertanika Journal of Tropical Agricultural Science, 48 (3). pp. 867-877. ISSN 1511-3701; eISSN: 2231-8542 http://pertanika2.upm.edu.my/pjtas/browse/regular-issue?article=JTAS-3205-2024 10.47836/pjtas.48.3.12 |
| spellingShingle | Shahril Agus, Syahir Aiman Shamsul-Bahri, Nurul Afiqah Juliana Syafinaz, . Mohd Sadali, Muhammad Farris Hazilawati Hamzah, . Mustaffa-Kamal, Farina Effect of N, N-dimethylglycine (DMG) supplementation on haematological parameters and frequency of CD4+ and CD8+ T cells in cats post-vaccination |
| title | Effect of N, N-dimethylglycine (DMG) supplementation on haematological parameters and frequency of CD4+ and CD8+ T cells in cats post-vaccination |
| title_full | Effect of N, N-dimethylglycine (DMG) supplementation on haematological parameters and frequency of CD4+ and CD8+ T cells in cats post-vaccination |
| title_fullStr | Effect of N, N-dimethylglycine (DMG) supplementation on haematological parameters and frequency of CD4+ and CD8+ T cells in cats post-vaccination |
| title_full_unstemmed | Effect of N, N-dimethylglycine (DMG) supplementation on haematological parameters and frequency of CD4+ and CD8+ T cells in cats post-vaccination |
| title_short | Effect of N, N-dimethylglycine (DMG) supplementation on haematological parameters and frequency of CD4+ and CD8+ T cells in cats post-vaccination |
| title_sort | effect of n, n-dimethylglycine (dmg) supplementation on haematological parameters and frequency of cd4+ and cd8+ t cells in cats post-vaccination |
| url | http://psasir.upm.edu.my/id/eprint/120899/ http://psasir.upm.edu.my/id/eprint/120899/ http://psasir.upm.edu.my/id/eprint/120899/ http://psasir.upm.edu.my/id/eprint/120899/1/120899.pdf |