Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase
The fed-batch cultivation process for the production of the recombinant protein, superoxide dismutase (SOD) by E. coli BL21 (DE3) pLysS was carried out. The cultivation process for recombinant SOD (ESOD) production was optimized through several approaches and strategies. The first approach was to...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/12060/ http://psasir.upm.edu.my/id/eprint/12060/1/IB_2009_17_A.pdf |
| _version_ | 1848841740684886016 |
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| author | Wan Omar, Wan Siti Atikah |
| author_facet | Wan Omar, Wan Siti Atikah |
| author_sort | Wan Omar, Wan Siti Atikah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The fed-batch cultivation process for the production of the recombinant protein,
superoxide dismutase (SOD) by E. coli BL21 (DE3) pLysS was carried out. The
cultivation process for recombinant SOD (ESOD) production was optimized through
several approaches and strategies. The first approach was to optimize medium and
culture conditions of the ESOD culture in shake flask via conventional and the
Response Surface Methodology (RSM) methods. Laboratory scale batch cultivation of
ESOD was then performed using a 2 L stirred tank bioreactor (STB) in order to further
optimize the medium and culture conditions. The effects of glucose concentrations (15
and 20 g/L), agitation speeds (300 – 1000 rpm) and controlled dissolved oxygen tension
(DOT) via agitation speeds (20%, 50% and 80%) on the growth performance of the
recombinant E. coli strain were investigated. In the final stage, fed-batch techniques were applied to the process for the development of high cell density cultivation. The
performance and kinetics of the ESOD fed-batch and batch cultivations were then
evaluated and compared. |
| first_indexed | 2025-11-15T07:48:03Z |
| format | Thesis |
| id | upm-12060 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:48:03Z |
| publishDate | 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-120602013-05-27T07:50:57Z http://psasir.upm.edu.my/id/eprint/12060/ Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase Wan Omar, Wan Siti Atikah The fed-batch cultivation process for the production of the recombinant protein, superoxide dismutase (SOD) by E. coli BL21 (DE3) pLysS was carried out. The cultivation process for recombinant SOD (ESOD) production was optimized through several approaches and strategies. The first approach was to optimize medium and culture conditions of the ESOD culture in shake flask via conventional and the Response Surface Methodology (RSM) methods. Laboratory scale batch cultivation of ESOD was then performed using a 2 L stirred tank bioreactor (STB) in order to further optimize the medium and culture conditions. The effects of glucose concentrations (15 and 20 g/L), agitation speeds (300 – 1000 rpm) and controlled dissolved oxygen tension (DOT) via agitation speeds (20%, 50% and 80%) on the growth performance of the recombinant E. coli strain were investigated. In the final stage, fed-batch techniques were applied to the process for the development of high cell density cultivation. The performance and kinetics of the ESOD fed-batch and batch cultivations were then evaluated and compared. 2009-10 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12060/1/IB_2009_17_A.pdf Wan Omar, Wan Siti Atikah (2009) Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase. Masters thesis, Universiti Putra Malaysia. Cultivation Escherichia coli Superoxide dismutase English |
| spellingShingle | Cultivation Escherichia coli Superoxide dismutase Wan Omar, Wan Siti Atikah Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase |
| title | Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase
|
| title_full | Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase
|
| title_fullStr | Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase
|
| title_full_unstemmed | Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase
|
| title_short | Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase
|
| title_sort | development of fed-batch cultivation process for esherichia coli harboring superoxide dismutase |
| topic | Cultivation Escherichia coli Superoxide dismutase |
| url | http://psasir.upm.edu.my/id/eprint/12060/ http://psasir.upm.edu.my/id/eprint/12060/1/IB_2009_17_A.pdf |