Ethanol production from lignocellulosic waste materials: kinetics and optimization studies

This study investigates the composition, hydrolysis, fermentation, kinetic studies and optimization by response surface methodology (RSM) of ten different lignocellulosic materials in ethanol production using enzymatic hydrolysis of isolated Trichoderma reesei and Aspergillus niger and fermentation...

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Main Authors: Ibrahim, Naeemah A., Majeed, Halah Hameed, Abid, Rand A., Alsultan, G. Abdulkareem, Mijan, N. Asikin, Lee, H. V., Kurniawan, Tonni Agustiono, Taufiq-Yap, Yun Hin
Format: Article
Language:English
Published: Royal Society of Chemistry 2025
Online Access:http://psasir.upm.edu.my/id/eprint/120475/
http://psasir.upm.edu.my/id/eprint/120475/1/120475.pdf
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author Ibrahim, Naeemah A.
Majeed, Halah Hameed
Abid, Rand A.
Alsultan, G. Abdulkareem
Mijan, N. Asikin
Lee, H. V.
Kurniawan, Tonni Agustiono
Taufiq-Yap, Yun Hin
author_facet Ibrahim, Naeemah A.
Majeed, Halah Hameed
Abid, Rand A.
Alsultan, G. Abdulkareem
Mijan, N. Asikin
Lee, H. V.
Kurniawan, Tonni Agustiono
Taufiq-Yap, Yun Hin
author_sort Ibrahim, Naeemah A.
building UPM Institutional Repository
collection Online Access
description This study investigates the composition, hydrolysis, fermentation, kinetic studies and optimization by response surface methodology (RSM) of ten different lignocellulosic materials in ethanol production using enzymatic hydrolysis of isolated Trichoderma reesei and Aspergillus niger and fermentation by Zymomonas mobilis and Saccharomyces cerevisiae. Proximate and ultimate analyses reveal that sugarcane bagasse and rice husk are ideal feedstocks due to their high volatile matter, low moisture, and ash content, offering more fermentable carbohydrates. The highest glucose concentrations were achieved from sugarcane bagasse (0.5689 g L−1) using T. reesei and from rice husk (0.5803 g L−1) using A. niger. Pretreatment increased glucose yields, with rice husk (RHAn) yielding 9.3 g L−1 ethanol in 60 h and sugarcane bagasse (SBTr) yielding 8.1 g L−1 in 48 h, and the particle size reduction to 75 μm enhanced glucose yields due to increased surface area. Kinetic models, including the Monod and Michaelis-Menten models, were used to describe ethanol production, with RHAn exhibiting the highest growth parameters. This study reports optimized ethanol production that achieved maximum yields under controlled conditions, further supporting the feasibility of large-scale bioethanol production.
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institution Universiti Putra Malaysia
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spelling upm-1204752025-10-02T07:30:41Z http://psasir.upm.edu.my/id/eprint/120475/ Ethanol production from lignocellulosic waste materials: kinetics and optimization studies Ibrahim, Naeemah A. Majeed, Halah Hameed Abid, Rand A. Alsultan, G. Abdulkareem Mijan, N. Asikin Lee, H. V. Kurniawan, Tonni Agustiono Taufiq-Yap, Yun Hin This study investigates the composition, hydrolysis, fermentation, kinetic studies and optimization by response surface methodology (RSM) of ten different lignocellulosic materials in ethanol production using enzymatic hydrolysis of isolated Trichoderma reesei and Aspergillus niger and fermentation by Zymomonas mobilis and Saccharomyces cerevisiae. Proximate and ultimate analyses reveal that sugarcane bagasse and rice husk are ideal feedstocks due to their high volatile matter, low moisture, and ash content, offering more fermentable carbohydrates. The highest glucose concentrations were achieved from sugarcane bagasse (0.5689 g L−1) using T. reesei and from rice husk (0.5803 g L−1) using A. niger. Pretreatment increased glucose yields, with rice husk (RHAn) yielding 9.3 g L−1 ethanol in 60 h and sugarcane bagasse (SBTr) yielding 8.1 g L−1 in 48 h, and the particle size reduction to 75 μm enhanced glucose yields due to increased surface area. Kinetic models, including the Monod and Michaelis-Menten models, were used to describe ethanol production, with RHAn exhibiting the highest growth parameters. This study reports optimized ethanol production that achieved maximum yields under controlled conditions, further supporting the feasibility of large-scale bioethanol production. Royal Society of Chemistry 2025 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/120475/1/120475.pdf Ibrahim, Naeemah A. and Majeed, Halah Hameed and Abid, Rand A. and Alsultan, G. Abdulkareem and Mijan, N. Asikin and Lee, H. V. and Kurniawan, Tonni Agustiono and Taufiq-Yap, Yun Hin (2025) Ethanol production from lignocellulosic waste materials: kinetics and optimization studies. RSC Advances, 15 (32). pp. 26091-26103. ISSN 2046-2069 https://pubs.rsc.org/en/content/articlelanding/2025/ra/d5ra02272j 10.1039/d5ra02272j
spellingShingle Ibrahim, Naeemah A.
Majeed, Halah Hameed
Abid, Rand A.
Alsultan, G. Abdulkareem
Mijan, N. Asikin
Lee, H. V.
Kurniawan, Tonni Agustiono
Taufiq-Yap, Yun Hin
Ethanol production from lignocellulosic waste materials: kinetics and optimization studies
title Ethanol production from lignocellulosic waste materials: kinetics and optimization studies
title_full Ethanol production from lignocellulosic waste materials: kinetics and optimization studies
title_fullStr Ethanol production from lignocellulosic waste materials: kinetics and optimization studies
title_full_unstemmed Ethanol production from lignocellulosic waste materials: kinetics and optimization studies
title_short Ethanol production from lignocellulosic waste materials: kinetics and optimization studies
title_sort ethanol production from lignocellulosic waste materials: kinetics and optimization studies
url http://psasir.upm.edu.my/id/eprint/120475/
http://psasir.upm.edu.my/id/eprint/120475/
http://psasir.upm.edu.my/id/eprint/120475/
http://psasir.upm.edu.my/id/eprint/120475/1/120475.pdf