The current landscape of CRISPR-Cas9-based technologies for imaging of genomic loci in fixed and live cells
Visualization of the spatiotemporal organization and dynamics of the genome in the nucleus is essential to elucidate how chromatin structure and nuclear function are intertwined. In the past, such studies were limited by the lack of appropriate tools. However, various imaging techniques now allow di...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Xia and He Publishing Inc.
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120430/ http://psasir.upm.edu.my/id/eprint/120430/1/120430.pdf |
| _version_ | 1848868185584959488 |
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| author | Yu, Choo Yee Yean, Chan Yean Chan, Kok Gan Ang, Geik Yong |
| author_facet | Yu, Choo Yee Yean, Chan Yean Chan, Kok Gan Ang, Geik Yong |
| author_sort | Yu, Choo Yee |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Visualization of the spatiotemporal organization and dynamics of the genome in the nucleus is essential to elucidate how chromatin structure and nuclear function are intertwined. In the past, such studies were limited by the lack of appropriate tools. However, various imaging techniques now allow direct visualization of genomic loci in living cells. One of the main drivers behind this progress is the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) technology. Initially used as an efficient and specific genome editing tool, the versatility of the CRISPR/Cas system has rapidly expanded its application range to include diagnostics, therapeutics, and, more recently, live cell imaging. In contrast to traditional fluorescent in situ hybridization, CRISPR/Cas-based imaging systems enable real-time dynamic tracking of genomic sequences down to the level of single cells and even a single locus. Recognizing the vital role played by CRISPR/ Cas-based imaging systems in advancing our understanding of genome function in relation to its spatiotemporal organization, this review aimed to depict the current landscape of CRISPR/Cas-based technologies in genomic imaging. It discusses the principles, notable features, advantages, and limitations of these CRISPR/Cas-based imaging systems and concludes with a brief outlook into the future. |
| first_indexed | 2025-11-15T14:48:22Z |
| format | Article |
| id | upm-120430 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:48:22Z |
| publishDate | 2024 |
| publisher | Xia and He Publishing Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1204302025-10-01T08:19:08Z http://psasir.upm.edu.my/id/eprint/120430/ The current landscape of CRISPR-Cas9-based technologies for imaging of genomic loci in fixed and live cells Yu, Choo Yee Yean, Chan Yean Chan, Kok Gan Ang, Geik Yong Visualization of the spatiotemporal organization and dynamics of the genome in the nucleus is essential to elucidate how chromatin structure and nuclear function are intertwined. In the past, such studies were limited by the lack of appropriate tools. However, various imaging techniques now allow direct visualization of genomic loci in living cells. One of the main drivers behind this progress is the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) technology. Initially used as an efficient and specific genome editing tool, the versatility of the CRISPR/Cas system has rapidly expanded its application range to include diagnostics, therapeutics, and, more recently, live cell imaging. In contrast to traditional fluorescent in situ hybridization, CRISPR/Cas-based imaging systems enable real-time dynamic tracking of genomic sequences down to the level of single cells and even a single locus. Recognizing the vital role played by CRISPR/ Cas-based imaging systems in advancing our understanding of genome function in relation to its spatiotemporal organization, this review aimed to depict the current landscape of CRISPR/Cas-based technologies in genomic imaging. It discusses the principles, notable features, advantages, and limitations of these CRISPR/Cas-based imaging systems and concludes with a brief outlook into the future. Xia and He Publishing Inc. 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/120430/1/120430.pdf Yu, Choo Yee and Yean, Chan Yean and Chan, Kok Gan and Ang, Geik Yong (2024) The current landscape of CRISPR-Cas9-based technologies for imaging of genomic loci in fixed and live cells. Gene Expression The Journal of Liver Research, 23 (3). art. no. undefined. pp. 211-222. ISSN eISSN: 1555-3884 https://www.xiahepublishing.com/1555-3884/GE-2024-00035 10.14218/ge.2024.00035 |
| spellingShingle | Yu, Choo Yee Yean, Chan Yean Chan, Kok Gan Ang, Geik Yong The current landscape of CRISPR-Cas9-based technologies for imaging of genomic loci in fixed and live cells |
| title | The current landscape of CRISPR-Cas9-based technologies for imaging of genomic loci in fixed and live cells |
| title_full | The current landscape of CRISPR-Cas9-based technologies for imaging of genomic loci in fixed and live cells |
| title_fullStr | The current landscape of CRISPR-Cas9-based technologies for imaging of genomic loci in fixed and live cells |
| title_full_unstemmed | The current landscape of CRISPR-Cas9-based technologies for imaging of genomic loci in fixed and live cells |
| title_short | The current landscape of CRISPR-Cas9-based technologies for imaging of genomic loci in fixed and live cells |
| title_sort | current landscape of crispr-cas9-based technologies for imaging of genomic loci in fixed and live cells |
| url | http://psasir.upm.edu.my/id/eprint/120430/ http://psasir.upm.edu.my/id/eprint/120430/ http://psasir.upm.edu.my/id/eprint/120430/ http://psasir.upm.edu.my/id/eprint/120430/1/120430.pdf |