Temporal dynamics of chicken host’s molecular response against Fowl adenovirus serotype 8b infection via RNA-sequencing
Background: Fowl adenovirus serotype 8b (FAdV-8b) is the etiological agent of inclusion body hepatitis’ outbreaks in chicken farms worldwide and a threat to the poultry industry. The isolation and identification of isolates of this pathogen are abundant, yet the pathogenesis and subsequent molecular...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BioMed Central
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120380/ http://psasir.upm.edu.my/id/eprint/120380/1/120380.pdf |
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| author | Azli, Bahiyah Salim, Nur Farhana Hair-Bejo, Mohd Omar, Abdul Rahman Mohamed Sohaimi, Norfitriah Clifford, Ugwu Chidozie Abdul Hamid, Nor Asilah Wati Mat Isa, Nurulfiza |
| author_facet | Azli, Bahiyah Salim, Nur Farhana Hair-Bejo, Mohd Omar, Abdul Rahman Mohamed Sohaimi, Norfitriah Clifford, Ugwu Chidozie Abdul Hamid, Nor Asilah Wati Mat Isa, Nurulfiza |
| author_sort | Azli, Bahiyah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Fowl adenovirus serotype 8b (FAdV-8b) is the etiological agent of inclusion body hepatitis’ outbreaks in chicken farms worldwide and a threat to the poultry industry. The isolation and identification of isolates of this pathogen are abundant, yet the pathogenesis and subsequent molecular mechanism are significantly understudied. Hence, this study aims to identify the differential gene expression profile of specific pathogen-free chicken livers infected with FAdV-8b strain UPMT1901 isolate. Results: The harvested chicken livers were subjected to biological pooling and transcriptomic profiling via RNA-sequencing, according to the days of post-infections (dpi). A total of 21,662 genes were identified in both control and infected groups, enriched in various biological processes and pathways, displaying patterns of disease progressions. The transcriptome analysis results revealed a three-fold reduced pattern of gene regulation with 9146, 7335, and 3800 significant differential expressed genes (DEGs) at 2-, 5-, and 7-dpi (dpi), respectively. Conclusion: These findings elucidate insights on the holistic disease progression of FAdV-8b in liver, from viral incubation and replication at 0–2-dpi, major metabolite hijacking at 3–5-dpi, followed by constant regulation of immune response at 6- and 7-dpi. Based on intensive literature review conducted prior to this study, this study is considered as the first study of the serotype 8b infection pathogenesis at the target organ, providing a better understanding and wide basis of pathogen-host interaction in FAdV-8b in chickens and its progression. |
| first_indexed | 2025-11-15T14:48:11Z |
| format | Article |
| id | upm-120380 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:48:11Z |
| publishDate | 2025 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1203802025-10-01T02:18:33Z http://psasir.upm.edu.my/id/eprint/120380/ Temporal dynamics of chicken host’s molecular response against Fowl adenovirus serotype 8b infection via RNA-sequencing Azli, Bahiyah Salim, Nur Farhana Hair-Bejo, Mohd Omar, Abdul Rahman Mohamed Sohaimi, Norfitriah Clifford, Ugwu Chidozie Abdul Hamid, Nor Asilah Wati Mat Isa, Nurulfiza Background: Fowl adenovirus serotype 8b (FAdV-8b) is the etiological agent of inclusion body hepatitis’ outbreaks in chicken farms worldwide and a threat to the poultry industry. The isolation and identification of isolates of this pathogen are abundant, yet the pathogenesis and subsequent molecular mechanism are significantly understudied. Hence, this study aims to identify the differential gene expression profile of specific pathogen-free chicken livers infected with FAdV-8b strain UPMT1901 isolate. Results: The harvested chicken livers were subjected to biological pooling and transcriptomic profiling via RNA-sequencing, according to the days of post-infections (dpi). A total of 21,662 genes were identified in both control and infected groups, enriched in various biological processes and pathways, displaying patterns of disease progressions. The transcriptome analysis results revealed a three-fold reduced pattern of gene regulation with 9146, 7335, and 3800 significant differential expressed genes (DEGs) at 2-, 5-, and 7-dpi (dpi), respectively. Conclusion: These findings elucidate insights on the holistic disease progression of FAdV-8b in liver, from viral incubation and replication at 0–2-dpi, major metabolite hijacking at 3–5-dpi, followed by constant regulation of immune response at 6- and 7-dpi. Based on intensive literature review conducted prior to this study, this study is considered as the first study of the serotype 8b infection pathogenesis at the target organ, providing a better understanding and wide basis of pathogen-host interaction in FAdV-8b in chickens and its progression. BioMed Central 2025 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/120380/1/120380.pdf Azli, Bahiyah and Salim, Nur Farhana and Hair-Bejo, Mohd and Omar, Abdul Rahman and Mohamed Sohaimi, Norfitriah and Clifford, Ugwu Chidozie and Abdul Hamid, Nor Asilah Wati and Mat Isa, Nurulfiza (2025) Temporal dynamics of chicken host’s molecular response against Fowl adenovirus serotype 8b infection via RNA-sequencing. BMC Genomics, 26 (1). art. no. 690. pp. 1-23. ISSN 1471-2164 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-025-11853-x 10.1186/s12864-025-11853-x |
| spellingShingle | Azli, Bahiyah Salim, Nur Farhana Hair-Bejo, Mohd Omar, Abdul Rahman Mohamed Sohaimi, Norfitriah Clifford, Ugwu Chidozie Abdul Hamid, Nor Asilah Wati Mat Isa, Nurulfiza Temporal dynamics of chicken host’s molecular response against Fowl adenovirus serotype 8b infection via RNA-sequencing |
| title | Temporal dynamics of chicken host’s molecular response against Fowl adenovirus serotype 8b infection via RNA-sequencing |
| title_full | Temporal dynamics of chicken host’s molecular response against Fowl adenovirus serotype 8b infection via RNA-sequencing |
| title_fullStr | Temporal dynamics of chicken host’s molecular response against Fowl adenovirus serotype 8b infection via RNA-sequencing |
| title_full_unstemmed | Temporal dynamics of chicken host’s molecular response against Fowl adenovirus serotype 8b infection via RNA-sequencing |
| title_short | Temporal dynamics of chicken host’s molecular response against Fowl adenovirus serotype 8b infection via RNA-sequencing |
| title_sort | temporal dynamics of chicken host’s molecular response against fowl adenovirus serotype 8b infection via rna-sequencing |
| url | http://psasir.upm.edu.my/id/eprint/120380/ http://psasir.upm.edu.my/id/eprint/120380/ http://psasir.upm.edu.my/id/eprint/120380/ http://psasir.upm.edu.my/id/eprint/120380/1/120380.pdf |