Further insights into the pathogenic mechanisms of haemotropic Mycoplasma ovis
In this study, we examined the effects of experimental intraperitoneal infection with haemotropic Mycoplasma ovis (0.5ml of blood containing 80% parasitaemia) on selected serum biomarkers and cellular pathology in mice. After infection, M. ovis cells appeared in the blood films within one week. A do...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Sains Malaysia,Universiti Sains Malaysia Press
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120365/ http://psasir.upm.edu.my/id/eprint/120365/1/120365.pdf |
| _version_ | 1848868169726296064 |
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| author | Thlama, Bura Paul Mohd Lila, Mohd Azmi Che' Amat, Azlan Chung, Eric Lim Teik Kamaludeen, Juriah Abdullah, Faez Firdaus Jesse |
| author_facet | Thlama, Bura Paul Mohd Lila, Mohd Azmi Che' Amat, Azlan Chung, Eric Lim Teik Kamaludeen, Juriah Abdullah, Faez Firdaus Jesse |
| author_sort | Thlama, Bura Paul |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, we examined the effects of experimental intraperitoneal infection with haemotropic Mycoplasma ovis (0.5ml of blood containing 80% parasitaemia) on selected serum biomarkers and cellular pathology in mice. After infection, M. ovis cells appeared in the blood films within one week. A dose-dependent peak of parasitemia was observed during the 3rd-week post-infection (pi), with a significant decrease in mean PCV between treatment versus control group at week 3 (t14 = -3.693, P < 0.02), week 5 (t14 = -2.096, P = 0.055), and week 7 (t14 = - 4.329, P = 0.001). There was a significantly (t8 = - 2.330, P = 0.048) lower serum oestrogen in treatment (10.38 ± 5.07) than control (17.43 ± 4.48), while serum progesterone was significantly (t8 = 5.415, P = 0.001) increased in treatment (27.37 ± 2.17) than control (15.92 ± 4.20). Serum haptoglobin was significantly (t8 = 8.525, P < 0.01) lower in treatment (8.72 ± 1.49) than control (18.16 ± 1.98) while the SAA was significantly (t8 = 3.362, P = 0.01) higher in treatment (16.79 ± 2.71) than control (11.59 ± 2.15). Prominent lesions observed in the ovary include degeneration, necrosis, vacuolation, and hypertrophy of the lutein cells in corpora lutea. In the lymph nodes, diffused cellular hyperplasia of the lymphoid tissue in the cortex. In the liver, degeneration and necrosis accompanied by leucocytic cellular infiltration and Kupffer cell proliferation within the sinusoids. There were diffused leucocytic infiltrations and proliferative lesions in the glomerulus of the kidneys. The disturbance in progesterone and ovarian pathology recorded here is a novel aspect of this study that highlights the potential role of haemotropic M. ovis in reproductive disorders. The observed changes in biomarkers and cellular reactions following M. ovis infection in the mouse may be further advanced in sheep and goats. |
| first_indexed | 2025-11-15T14:48:07Z |
| format | Article |
| id | upm-120365 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:48:07Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Sains Malaysia,Universiti Sains Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1203652025-10-01T01:36:13Z http://psasir.upm.edu.my/id/eprint/120365/ Further insights into the pathogenic mechanisms of haemotropic Mycoplasma ovis Thlama, Bura Paul Mohd Lila, Mohd Azmi Che' Amat, Azlan Chung, Eric Lim Teik Kamaludeen, Juriah Abdullah, Faez Firdaus Jesse In this study, we examined the effects of experimental intraperitoneal infection with haemotropic Mycoplasma ovis (0.5ml of blood containing 80% parasitaemia) on selected serum biomarkers and cellular pathology in mice. After infection, M. ovis cells appeared in the blood films within one week. A dose-dependent peak of parasitemia was observed during the 3rd-week post-infection (pi), with a significant decrease in mean PCV between treatment versus control group at week 3 (t14 = -3.693, P < 0.02), week 5 (t14 = -2.096, P = 0.055), and week 7 (t14 = - 4.329, P = 0.001). There was a significantly (t8 = - 2.330, P = 0.048) lower serum oestrogen in treatment (10.38 ± 5.07) than control (17.43 ± 4.48), while serum progesterone was significantly (t8 = 5.415, P = 0.001) increased in treatment (27.37 ± 2.17) than control (15.92 ± 4.20). Serum haptoglobin was significantly (t8 = 8.525, P < 0.01) lower in treatment (8.72 ± 1.49) than control (18.16 ± 1.98) while the SAA was significantly (t8 = 3.362, P = 0.01) higher in treatment (16.79 ± 2.71) than control (11.59 ± 2.15). Prominent lesions observed in the ovary include degeneration, necrosis, vacuolation, and hypertrophy of the lutein cells in corpora lutea. In the lymph nodes, diffused cellular hyperplasia of the lymphoid tissue in the cortex. In the liver, degeneration and necrosis accompanied by leucocytic cellular infiltration and Kupffer cell proliferation within the sinusoids. There were diffused leucocytic infiltrations and proliferative lesions in the glomerulus of the kidneys. The disturbance in progesterone and ovarian pathology recorded here is a novel aspect of this study that highlights the potential role of haemotropic M. ovis in reproductive disorders. The observed changes in biomarkers and cellular reactions following M. ovis infection in the mouse may be further advanced in sheep and goats. Penerbit Universiti Sains Malaysia,Universiti Sains Malaysia Press 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/120365/1/120365.pdf Thlama, Bura Paul and Mohd Lila, Mohd Azmi and Che' Amat, Azlan and Chung, Eric Lim Teik and Kamaludeen, Juriah and Abdullah, Faez Firdaus Jesse (2024) Further insights into the pathogenic mechanisms of haemotropic Mycoplasma ovis. Tropical Life Sciences Research, 35 (3). pp. 319-337. ISSN 1985-3718; eISSN: 2180-4249 https://ejournal.usm.my/tlsr/article/view/tlsr_vol35-no-3-2024_15 10.21315/tlsr2024.35.3.15 |
| spellingShingle | Thlama, Bura Paul Mohd Lila, Mohd Azmi Che' Amat, Azlan Chung, Eric Lim Teik Kamaludeen, Juriah Abdullah, Faez Firdaus Jesse Further insights into the pathogenic mechanisms of haemotropic Mycoplasma ovis |
| title | Further insights into the pathogenic mechanisms of haemotropic Mycoplasma ovis |
| title_full | Further insights into the pathogenic mechanisms of haemotropic Mycoplasma ovis |
| title_fullStr | Further insights into the pathogenic mechanisms of haemotropic Mycoplasma ovis |
| title_full_unstemmed | Further insights into the pathogenic mechanisms of haemotropic Mycoplasma ovis |
| title_short | Further insights into the pathogenic mechanisms of haemotropic Mycoplasma ovis |
| title_sort | further insights into the pathogenic mechanisms of haemotropic mycoplasma ovis |
| url | http://psasir.upm.edu.my/id/eprint/120365/ http://psasir.upm.edu.my/id/eprint/120365/ http://psasir.upm.edu.my/id/eprint/120365/ http://psasir.upm.edu.my/id/eprint/120365/1/120365.pdf |