Effect of 660nm LED photobiomodulation on proliferation and chondrogenic of meniscus-derived stem cells (MeSCs)
Background: Meniscus-derived stem cells (MeSCs), a unique type of MSC, have outstanding advantages in meniscal cytotherapy and tissue engineering, but the effects and molecular mechanisms of PBM on MeSCs are still unclear. Methods: We used 660-nm LED light with different energy densities to irradiat...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120364/ http://psasir.upm.edu.my/id/eprint/120364/1/120364.pdf |
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| author | Ling, Mok Pooi Jiabei, Tong Ramasamy, Rajesh Subbiah, Suresh Kumar Wu, Xiaoyun You, Yanyan Wang, Jiaojiao Bei, Tong Jia Rampal, Sanjiv |
| author_facet | Ling, Mok Pooi Jiabei, Tong Ramasamy, Rajesh Subbiah, Suresh Kumar Wu, Xiaoyun You, Yanyan Wang, Jiaojiao Bei, Tong Jia Rampal, Sanjiv |
| author_sort | Ling, Mok Pooi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Meniscus-derived stem cells (MeSCs), a unique type of MSC, have outstanding advantages in meniscal cytotherapy and tissue engineering, but the effects and molecular mechanisms of PBM on MeSCs are still unclear. Methods: We used 660-nm LED light with different energy densities to irradiate six human MeSC samples and tested their proliferation rate via cell counting, chondrogenic differentiation capacity via the DMMB assay, mitochondrial activity via the MTT assay, and gene expression via qPCR. Results: The proliferation ability, chondrogenic capacity and mitochondrial activity of the 18 J/cm2 group were greater than those of the 4 J/cm2 and control groups. The mRNA expression levels of Akt, PI3K, TGFβ3, Ki67 and Notch-1 in the 18 J/cm2 group were greater than those in the other groups in most samples. After chondrogenic induction, the expression of Col2A1, Sox9 and Aggrecan in the 18 J/cm2 group was significantly greater than that in the 4 J/cm2 and control groups in most of the samples. The variation in the MTT values and Src, PI3K, Akt, mTOR and GSK3β levels decreased with time. Conclusion: The results showed that 660-nm LED red light promoted proliferation and chondrogenic differentiation and affected the gene expression of MeSCs, and the effects on gene expression and mitochondrial activity decreased with time. |
| first_indexed | 2025-11-15T14:48:07Z |
| format | Article |
| id | upm-120364 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:48:07Z |
| publishDate | 2024 |
| publisher | Nature Portfolio |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1203642025-10-01T01:33:30Z http://psasir.upm.edu.my/id/eprint/120364/ Effect of 660nm LED photobiomodulation on proliferation and chondrogenic of meniscus-derived stem cells (MeSCs) Ling, Mok Pooi Jiabei, Tong Ramasamy, Rajesh Subbiah, Suresh Kumar Wu, Xiaoyun You, Yanyan Wang, Jiaojiao Bei, Tong Jia Rampal, Sanjiv Background: Meniscus-derived stem cells (MeSCs), a unique type of MSC, have outstanding advantages in meniscal cytotherapy and tissue engineering, but the effects and molecular mechanisms of PBM on MeSCs are still unclear. Methods: We used 660-nm LED light with different energy densities to irradiate six human MeSC samples and tested their proliferation rate via cell counting, chondrogenic differentiation capacity via the DMMB assay, mitochondrial activity via the MTT assay, and gene expression via qPCR. Results: The proliferation ability, chondrogenic capacity and mitochondrial activity of the 18 J/cm2 group were greater than those of the 4 J/cm2 and control groups. The mRNA expression levels of Akt, PI3K, TGFβ3, Ki67 and Notch-1 in the 18 J/cm2 group were greater than those in the other groups in most samples. After chondrogenic induction, the expression of Col2A1, Sox9 and Aggrecan in the 18 J/cm2 group was significantly greater than that in the 4 J/cm2 and control groups in most of the samples. The variation in the MTT values and Src, PI3K, Akt, mTOR and GSK3β levels decreased with time. Conclusion: The results showed that 660-nm LED red light promoted proliferation and chondrogenic differentiation and affected the gene expression of MeSCs, and the effects on gene expression and mitochondrial activity decreased with time. Nature Portfolio 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/120364/1/120364.pdf Ling, Mok Pooi and Jiabei, Tong and Ramasamy, Rajesh and Subbiah, Suresh Kumar and Wu, Xiaoyun and You, Yanyan and Wang, Jiaojiao and Bei, Tong Jia and Rampal, Sanjiv (2024) Effect of 660nm LED photobiomodulation on proliferation and chondrogenic of meniscus-derived stem cells (MeSCs). Scientific Reports, 14. art. no. 19735. pp. 1-23. ISSN 2045-2322; eISSN: 2045-2322 https://www.nature.com/articles/s41598-024-70258-0#:~:text=The%20results%20showed%20that%20660,mitochondrial%20activity%20decreased%20with%20time. 10.1038/s41598-024-70258-0 |
| spellingShingle | Ling, Mok Pooi Jiabei, Tong Ramasamy, Rajesh Subbiah, Suresh Kumar Wu, Xiaoyun You, Yanyan Wang, Jiaojiao Bei, Tong Jia Rampal, Sanjiv Effect of 660nm LED photobiomodulation on proliferation and chondrogenic of meniscus-derived stem cells (MeSCs) |
| title | Effect of 660nm LED photobiomodulation on proliferation and chondrogenic of meniscus-derived stem cells (MeSCs) |
| title_full | Effect of 660nm LED photobiomodulation on proliferation and chondrogenic of meniscus-derived stem cells (MeSCs) |
| title_fullStr | Effect of 660nm LED photobiomodulation on proliferation and chondrogenic of meniscus-derived stem cells (MeSCs) |
| title_full_unstemmed | Effect of 660nm LED photobiomodulation on proliferation and chondrogenic of meniscus-derived stem cells (MeSCs) |
| title_short | Effect of 660nm LED photobiomodulation on proliferation and chondrogenic of meniscus-derived stem cells (MeSCs) |
| title_sort | effect of 660nm led photobiomodulation on proliferation and chondrogenic of meniscus-derived stem cells (mescs) |
| url | http://psasir.upm.edu.my/id/eprint/120364/ http://psasir.upm.edu.my/id/eprint/120364/ http://psasir.upm.edu.my/id/eprint/120364/ http://psasir.upm.edu.my/id/eprint/120364/1/120364.pdf |