Morphological and genetic identification of Halophila species and a new distribution record of Halophila nipponica at the Tanjung Adang Laut shoal, Johor, Malaysia
The Halophila species exhibit complex characteristics due to their high degree of variation across different bioregions. This study delves into the intricate characteristics of Halophila species in the Tanjung Adang Laut shoal, Johor, Malaysia, and offers valuable insights through morphological and...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120298/ http://psasir.upm.edu.my/id/eprint/120298/1/120298.pdf |
| _version_ | 1848868157111926784 |
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| author | Che Alias, Muhammad Afif Zakaria, Muta Harah Ramaiya, Shiamala Devi Esa, Yuzine Ab. Ghani, Nurul Izza Bujang, Japar Sidik |
| author_facet | Che Alias, Muhammad Afif Zakaria, Muta Harah Ramaiya, Shiamala Devi Esa, Yuzine Ab. Ghani, Nurul Izza Bujang, Japar Sidik |
| author_sort | Che Alias, Muhammad Afif |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The Halophila species exhibit complex characteristics due to their high degree of variation across different bioregions. This study delves into the intricate characteristics of Halophila species in the Tanjung Adang Laut shoal, Johor, Malaysia, and offers valuable insights through morphological and genetic evidence. Employing internal transcribed sequences (ITS), we investigated the phylogeny of Halophila species, revealing distinct clades for H. ovalis, H. major, H. spinulosa, and the newly recorded H. nipponica. Notably, H. nipponica from the Tanjung Adang Laut shoal formed a conspecific relationship with its counterparts from Japan and Korea (98.3-98.5% similarity; 5-11 bp differences). Morphologically, distinguishing features, including the ratio of the half-lamina width (1:4.76-6.13 mm) and crossvein count (4-7 pairs), supported the identification of H. nipponica. Genetic distance analyses revealed differences between H. nipponica, H. ovalis, and H. major, indicating haplotype diversity. Geographical variations were evident, as H. nipponica presented unique haplotypes (H24) in its clade. The 47 haplotypes network identified significant mutation sites, providing a comprehensive understanding of genetic and morphological distinctions. In conclusion, this study highlights the intricate characteristics and phylogeny of Halophila species in the Tanjung Adang Laut shoal, Johor, and provides valuable insights into their genetic and morphological diversity. |
| first_indexed | 2025-11-15T14:47:55Z |
| format | Article |
| id | upm-120298 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:47:55Z |
| publishDate | 2024 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1202982025-10-05T18:13:36Z http://psasir.upm.edu.my/id/eprint/120298/ Morphological and genetic identification of Halophila species and a new distribution record of Halophila nipponica at the Tanjung Adang Laut shoal, Johor, Malaysia Che Alias, Muhammad Afif Zakaria, Muta Harah Ramaiya, Shiamala Devi Esa, Yuzine Ab. Ghani, Nurul Izza Bujang, Japar Sidik The Halophila species exhibit complex characteristics due to their high degree of variation across different bioregions. This study delves into the intricate characteristics of Halophila species in the Tanjung Adang Laut shoal, Johor, Malaysia, and offers valuable insights through morphological and genetic evidence. Employing internal transcribed sequences (ITS), we investigated the phylogeny of Halophila species, revealing distinct clades for H. ovalis, H. major, H. spinulosa, and the newly recorded H. nipponica. Notably, H. nipponica from the Tanjung Adang Laut shoal formed a conspecific relationship with its counterparts from Japan and Korea (98.3-98.5% similarity; 5-11 bp differences). Morphologically, distinguishing features, including the ratio of the half-lamina width (1:4.76-6.13 mm) and crossvein count (4-7 pairs), supported the identification of H. nipponica. Genetic distance analyses revealed differences between H. nipponica, H. ovalis, and H. major, indicating haplotype diversity. Geographical variations were evident, as H. nipponica presented unique haplotypes (H24) in its clade. The 47 haplotypes network identified significant mutation sites, providing a comprehensive understanding of genetic and morphological distinctions. In conclusion, this study highlights the intricate characteristics and phylogeny of Halophila species in the Tanjung Adang Laut shoal, Johor, and provides valuable insights into their genetic and morphological diversity. Public Library of Science 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/120298/1/120298.pdf Che Alias, Muhammad Afif and Zakaria, Muta Harah and Ramaiya, Shiamala Devi and Esa, Yuzine and Ab. Ghani, Nurul Izza and Bujang, Japar Sidik (2024) Morphological and genetic identification of Halophila species and a new distribution record of Halophila nipponica at the Tanjung Adang Laut shoal, Johor, Malaysia. PLoS ONE, 19 (10 October). art. no. e0309143. ISSN eISSN: 1932-6203 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0309143 10.1371/journal.pone.0309143 |
| spellingShingle | Che Alias, Muhammad Afif Zakaria, Muta Harah Ramaiya, Shiamala Devi Esa, Yuzine Ab. Ghani, Nurul Izza Bujang, Japar Sidik Morphological and genetic identification of Halophila species and a new distribution record of Halophila nipponica at the Tanjung Adang Laut shoal, Johor, Malaysia |
| title | Morphological and genetic identification of Halophila species and a new distribution record of Halophila nipponica at the Tanjung Adang Laut shoal, Johor, Malaysia |
| title_full | Morphological and genetic identification of Halophila species and a new distribution record of Halophila nipponica at the Tanjung Adang Laut shoal, Johor, Malaysia |
| title_fullStr | Morphological and genetic identification of Halophila species and a new distribution record of Halophila nipponica at the Tanjung Adang Laut shoal, Johor, Malaysia |
| title_full_unstemmed | Morphological and genetic identification of Halophila species and a new distribution record of Halophila nipponica at the Tanjung Adang Laut shoal, Johor, Malaysia |
| title_short | Morphological and genetic identification of Halophila species and a new distribution record of Halophila nipponica at the Tanjung Adang Laut shoal, Johor, Malaysia |
| title_sort | morphological and genetic identification of halophila species and a new distribution record of halophila nipponica at the tanjung adang laut shoal, johor, malaysia |
| url | http://psasir.upm.edu.my/id/eprint/120298/ http://psasir.upm.edu.my/id/eprint/120298/ http://psasir.upm.edu.my/id/eprint/120298/ http://psasir.upm.edu.my/id/eprint/120298/1/120298.pdf |