Assessment of Opuntia ficus-indica supplementation on enhancing antioxidant levels

Opuntia ficus-indica (OFi) is a major fruit source prevalent in semiarid and arid regions across various countries worldwide. It is widely recognised for its potential health benefits; however, most studies investigating its effects have been limited to pre-clinical models, highlighting the need for...

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Main Authors: Zaman, Rahela, Sin, Eugenie Sing Tan, Ahmad Bustami, Normina, Amini, Farahnaz, Seghayat, Marjan Sadat, Yu, Bin Ho, Chung, Keat Tan
Format: Article
Language:English
Published: Nature Research 2025
Online Access:http://psasir.upm.edu.my/id/eprint/120218/
http://psasir.upm.edu.my/id/eprint/120218/1/120218.pdf
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author Zaman, Rahela
Sin, Eugenie Sing Tan
Ahmad Bustami, Normina
Amini, Farahnaz
Seghayat, Marjan Sadat
Yu, Bin Ho
Chung, Keat Tan
author_facet Zaman, Rahela
Sin, Eugenie Sing Tan
Ahmad Bustami, Normina
Amini, Farahnaz
Seghayat, Marjan Sadat
Yu, Bin Ho
Chung, Keat Tan
author_sort Zaman, Rahela
building UPM Institutional Repository
collection Online Access
description Opuntia ficus-indica (OFi) is a major fruit source prevalent in semiarid and arid regions across various countries worldwide. It is widely recognised for its potential health benefits; however, most studies investigating its effects have been limited to pre-clinical models, highlighting the need for further validation through clinical trials. This study aimed to evaluate the effectiveness of OFi supplementation in enhancing antioxidant levels. Fifty healthy participants, aged 18 years and older, including males and females, received a daily OFi supplement of 1500 mg for 3 months. These findings revealed a significant 48.1% increase in salivary total antioxidant capacity (TAC) (P < 0.001), indicating improved antioxidant activity. Simultaneously, oxidative stress biomarkers showed substantial reductions: malondialdehyde (MDA) decreased by 28.3%, nitrotyrosine (3-NT) decreased by 51.5%, and 8-hydroxy-2’-deoxyguanosine (8-OHdG) decreased by 59.8% (P < 0.001). Furthermore, participants reported a 20.1% improvement (P < 0.001) in Visual Analogue Scales (VAS), reflecting a notable enhancement in overall well-being. In conclusion, OFi exhibited promising efficacy in elevating antioxidant levels and mitigating oxidative stress. These findings suggest its potential as an adjuvant therapy for managing chronic conditions associated with oxidative stress.
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spelling upm-1202182025-09-25T07:06:55Z http://psasir.upm.edu.my/id/eprint/120218/ Assessment of Opuntia ficus-indica supplementation on enhancing antioxidant levels Zaman, Rahela Sin, Eugenie Sing Tan Ahmad Bustami, Normina Amini, Farahnaz Seghayat, Marjan Sadat Yu, Bin Ho Chung, Keat Tan Opuntia ficus-indica (OFi) is a major fruit source prevalent in semiarid and arid regions across various countries worldwide. It is widely recognised for its potential health benefits; however, most studies investigating its effects have been limited to pre-clinical models, highlighting the need for further validation through clinical trials. This study aimed to evaluate the effectiveness of OFi supplementation in enhancing antioxidant levels. Fifty healthy participants, aged 18 years and older, including males and females, received a daily OFi supplement of 1500 mg for 3 months. These findings revealed a significant 48.1% increase in salivary total antioxidant capacity (TAC) (P < 0.001), indicating improved antioxidant activity. Simultaneously, oxidative stress biomarkers showed substantial reductions: malondialdehyde (MDA) decreased by 28.3%, nitrotyrosine (3-NT) decreased by 51.5%, and 8-hydroxy-2’-deoxyguanosine (8-OHdG) decreased by 59.8% (P < 0.001). Furthermore, participants reported a 20.1% improvement (P < 0.001) in Visual Analogue Scales (VAS), reflecting a notable enhancement in overall well-being. In conclusion, OFi exhibited promising efficacy in elevating antioxidant levels and mitigating oxidative stress. These findings suggest its potential as an adjuvant therapy for managing chronic conditions associated with oxidative stress. Nature Research 2025 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/120218/1/120218.pdf Zaman, Rahela and Sin, Eugenie Sing Tan and Ahmad Bustami, Normina and Amini, Farahnaz and Seghayat, Marjan Sadat and Yu, Bin Ho and Chung, Keat Tan (2025) Assessment of Opuntia ficus-indica supplementation on enhancing antioxidant levels. Scientific Reports, 15 (1). art. no. 3507. pp. 1-11. ISSN 2045-2322 https://www.nature.com/articles/s41598-025-87680-7?error=cookies_not_supported&code=6cb54602-ae23-4402-86d1-018b57b6e979 10.1038/s41598-025-87680-7
spellingShingle Zaman, Rahela
Sin, Eugenie Sing Tan
Ahmad Bustami, Normina
Amini, Farahnaz
Seghayat, Marjan Sadat
Yu, Bin Ho
Chung, Keat Tan
Assessment of Opuntia ficus-indica supplementation on enhancing antioxidant levels
title Assessment of Opuntia ficus-indica supplementation on enhancing antioxidant levels
title_full Assessment of Opuntia ficus-indica supplementation on enhancing antioxidant levels
title_fullStr Assessment of Opuntia ficus-indica supplementation on enhancing antioxidant levels
title_full_unstemmed Assessment of Opuntia ficus-indica supplementation on enhancing antioxidant levels
title_short Assessment of Opuntia ficus-indica supplementation on enhancing antioxidant levels
title_sort assessment of opuntia ficus-indica supplementation on enhancing antioxidant levels
url http://psasir.upm.edu.my/id/eprint/120218/
http://psasir.upm.edu.my/id/eprint/120218/
http://psasir.upm.edu.my/id/eprint/120218/
http://psasir.upm.edu.my/id/eprint/120218/1/120218.pdf