Long-read sequencing and genome assembly of natural history collection samples and challenging specimens

Long-read sequencing and genome assembly of natural history collection samples and challenging specimens

Museum collections harbor millions of samples, largely unutilized for long-read sequencing. Here, we use ethanol-preserved samples containing kilobase-sized DNA to show that amplification-free protocols can yield contiguous genome assemblies. Additionally, using a modified amplification-based protoc...

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Bibliographic Details
Main Authors: Bein, Bernhard, Chrysostomakis, Ioannis, Arantes, Larissa S., Brown, Tom, Gerheim, Charlotte, Schell, Tilman, Schneider, Clément, Leushkin, Evgeny, Chen, Zeyuan, Sigwart, Julia, Gonzalez, Vanessa, Wong, Nur Leena W.S., Santos, Fabricio R., Blom, Mozes P.K., Mayer, Frieder, Mazzoni, Camila J., Böhne, Astrid, Winkler, Sylke, Greve, Carola, Hiller, Michael
Format: Article
Language:English
Published: BioMed Central 2025
Online Access:http://psasir.upm.edu.my/id/eprint/120192/
http://psasir.upm.edu.my/id/eprint/120192/1/120192.pdf

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