Effects of low glucose microenvironment on the proliferation, migration and senescence of meniscus-derived stem cells
Meniscus-derived stem cells (MeSCs) hold great promise for cytotherapy of the meniscus. Large-scale cell expansion in vitro is usually accompanied by decreased proliferation and migration and increased senescence, leading to a decrease in treatment efficiency. Therefore, the present study aimed to c...
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| Format: | Article |
| Language: | English |
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Nature Research
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120153/ http://psasir.upm.edu.my/id/eprint/120153/1/120153.pdf |
| _version_ | 1848868124882894848 |
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| author | Jiabei, Tong Alzahrani, Badr Subbiah, Suresh Kumar Rampal, Sanjiv Ramasamy, Rajesh Zhijie, Ma Xiaoyun, Wu Pooi, Ling Mok |
| author_facet | Jiabei, Tong Alzahrani, Badr Subbiah, Suresh Kumar Rampal, Sanjiv Ramasamy, Rajesh Zhijie, Ma Xiaoyun, Wu Pooi, Ling Mok |
| author_sort | Jiabei, Tong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Meniscus-derived stem cells (MeSCs) hold great promise for cytotherapy of the meniscus. Large-scale cell expansion in vitro is usually accompanied by decreased proliferation and migration and increased senescence, leading to a decrease in treatment efficiency. Therefore, the present study aimed to compare the effects of different concentrations of glucose on the proliferation, migration, senescence and protein expression of MeSCs. In this study, human MeSCs were cultured in two types of expansion media: low-glucose (LG) DMEM (5.5 mM glucose) and high-glucose (HG) DMEM (25 mM glucose). At specific passage number, the proliferation rate was evaluated by cell counting, the migration rate was evaluated by a scratch-wound assay, cell senescence was evaluated by β-GAL assays, and protein expression/phosphorylation was evaluated by ELISA. The results showed that a lower concentration of glucose promoted proliferation and migration, reduced cell senescence, and activated PI3K/Akt-related signaling pathways. This study provides a preliminary basis for the use of this medium composition for MeSC expansion. |
| first_indexed | 2025-11-15T14:47:25Z |
| format | Article |
| id | upm-120153 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:47:25Z |
| publishDate | 2025 |
| publisher | Nature Research |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1201532025-09-24T02:11:34Z http://psasir.upm.edu.my/id/eprint/120153/ Effects of low glucose microenvironment on the proliferation, migration and senescence of meniscus-derived stem cells Jiabei, Tong Alzahrani, Badr Subbiah, Suresh Kumar Rampal, Sanjiv Ramasamy, Rajesh Zhijie, Ma Xiaoyun, Wu Pooi, Ling Mok Meniscus-derived stem cells (MeSCs) hold great promise for cytotherapy of the meniscus. Large-scale cell expansion in vitro is usually accompanied by decreased proliferation and migration and increased senescence, leading to a decrease in treatment efficiency. Therefore, the present study aimed to compare the effects of different concentrations of glucose on the proliferation, migration, senescence and protein expression of MeSCs. In this study, human MeSCs were cultured in two types of expansion media: low-glucose (LG) DMEM (5.5 mM glucose) and high-glucose (HG) DMEM (25 mM glucose). At specific passage number, the proliferation rate was evaluated by cell counting, the migration rate was evaluated by a scratch-wound assay, cell senescence was evaluated by β-GAL assays, and protein expression/phosphorylation was evaluated by ELISA. The results showed that a lower concentration of glucose promoted proliferation and migration, reduced cell senescence, and activated PI3K/Akt-related signaling pathways. This study provides a preliminary basis for the use of this medium composition for MeSC expansion. Nature Research 2025 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/120153/1/120153.pdf Jiabei, Tong and Alzahrani, Badr and Subbiah, Suresh Kumar and Rampal, Sanjiv and Ramasamy, Rajesh and Zhijie, Ma and Xiaoyun, Wu and Pooi, Ling Mok (2025) Effects of low glucose microenvironment on the proliferation, migration and senescence of meniscus-derived stem cells. Scientific Reports, 15 (1). art. no. 23748. pp. 1-17. ISSN 2045-2322 https://www.nature.com/articles/s41598-025-07642-x?error=cookies_not_supported&code=02b885f8-4fa6-4019-aff5-400a5491261d 10.1038/s41598-025-07642-x |
| spellingShingle | Jiabei, Tong Alzahrani, Badr Subbiah, Suresh Kumar Rampal, Sanjiv Ramasamy, Rajesh Zhijie, Ma Xiaoyun, Wu Pooi, Ling Mok Effects of low glucose microenvironment on the proliferation, migration and senescence of meniscus-derived stem cells |
| title | Effects of low glucose microenvironment on the proliferation, migration and senescence of meniscus-derived stem cells |
| title_full | Effects of low glucose microenvironment on the proliferation, migration and senescence of meniscus-derived stem cells |
| title_fullStr | Effects of low glucose microenvironment on the proliferation, migration and senescence of meniscus-derived stem cells |
| title_full_unstemmed | Effects of low glucose microenvironment on the proliferation, migration and senescence of meniscus-derived stem cells |
| title_short | Effects of low glucose microenvironment on the proliferation, migration and senescence of meniscus-derived stem cells |
| title_sort | effects of low glucose microenvironment on the proliferation, migration and senescence of meniscus-derived stem cells |
| url | http://psasir.upm.edu.my/id/eprint/120153/ http://psasir.upm.edu.my/id/eprint/120153/ http://psasir.upm.edu.my/id/eprint/120153/ http://psasir.upm.edu.my/id/eprint/120153/1/120153.pdf |