Characterisation and expression analyses of two putative cerato-platanin proteins isolated from Ganoderma boninense Pat.
Cerato-platanin (CP) proteins are small extracellular proteins that play essential roles in the plant-pathogen interactions of some economically important filamentous fungi. At present, there is a lack of information about the roles of CP proteins secreted by Ganoderma boninense, the causal agent of...
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| Format: | Article |
| Language: | English |
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Academic Press
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120098/ http://psasir.upm.edu.my/id/eprint/120098/1/120098.pdf |
| _version_ | 1848868112299982848 |
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| author | Hamid, Sathyapriya Chai, Ling Ho Abdullah, Siti Nor Akmar Ti, Eng Leslie Low Nagappan, Jayanthi Mui, Yun Wong |
| author_facet | Hamid, Sathyapriya Chai, Ling Ho Abdullah, Siti Nor Akmar Ti, Eng Leslie Low Nagappan, Jayanthi Mui, Yun Wong |
| author_sort | Hamid, Sathyapriya |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Cerato-platanin (CP) proteins are small extracellular proteins that play essential roles in the plant-pathogen interactions of some economically important filamentous fungi. At present, there is a lack of information about the roles of CP proteins secreted by Ganoderma boninense, the causal agent of basal stem rot disease in African oil palm, during the early host-pathogen interaction. This work studies the properties and roles of two CP proteins from G. boninense strain PER71. Using bioassay-guided fractionation and nano-LC-MS/MS analysis, we identified peptide matches for G. sinense ZZ0214-1 CP-like and other elicitor proteins in the culture filtrate of G. boninense strain PER71. We found that GbCP2 and GbCP6, two out of nine CP gene models identified in the fungal genome of G. boninense strain PER71, exhibited potential effector properties. We further validated their effector properties by isolating and characterising their coding regions. Interestingly, GbCP6 showed a significant expression level at 11 days post inoculation, indicating its significance in fungal pathogenesis. On the other hand, GbCP2 did not show differential expression at any sampling point, suggesting a different function or role in the pathogenicity of G. boninense. This study identifies GbCP6 as a potential biomarker for early detection of basal stem rot disease in oil palm. |
| first_indexed | 2025-11-15T14:47:13Z |
| format | Article |
| id | upm-120098 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:47:13Z |
| publishDate | 2024 |
| publisher | Academic Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1200982025-09-23T04:04:08Z http://psasir.upm.edu.my/id/eprint/120098/ Characterisation and expression analyses of two putative cerato-platanin proteins isolated from Ganoderma boninense Pat. Hamid, Sathyapriya Chai, Ling Ho Abdullah, Siti Nor Akmar Ti, Eng Leslie Low Nagappan, Jayanthi Mui, Yun Wong Cerato-platanin (CP) proteins are small extracellular proteins that play essential roles in the plant-pathogen interactions of some economically important filamentous fungi. At present, there is a lack of information about the roles of CP proteins secreted by Ganoderma boninense, the causal agent of basal stem rot disease in African oil palm, during the early host-pathogen interaction. This work studies the properties and roles of two CP proteins from G. boninense strain PER71. Using bioassay-guided fractionation and nano-LC-MS/MS analysis, we identified peptide matches for G. sinense ZZ0214-1 CP-like and other elicitor proteins in the culture filtrate of G. boninense strain PER71. We found that GbCP2 and GbCP6, two out of nine CP gene models identified in the fungal genome of G. boninense strain PER71, exhibited potential effector properties. We further validated their effector properties by isolating and characterising their coding regions. Interestingly, GbCP6 showed a significant expression level at 11 days post inoculation, indicating its significance in fungal pathogenesis. On the other hand, GbCP2 did not show differential expression at any sampling point, suggesting a different function or role in the pathogenicity of G. boninense. This study identifies GbCP6 as a potential biomarker for early detection of basal stem rot disease in oil palm. Academic Press 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/120098/1/120098.pdf Hamid, Sathyapriya and Chai, Ling Ho and Abdullah, Siti Nor Akmar and Ti, Eng Leslie Low and Nagappan, Jayanthi and Mui, Yun Wong (2024) Characterisation and expression analyses of two putative cerato-platanin proteins isolated from Ganoderma boninense Pat. Physiological and Molecular Plant Pathology, 131. art. no. 102289. pp. 1-12. ISSN 0885-5765; eISSN: 1096-1178 https://linkinghub.elsevier.com/retrieve/pii/S0885576524000730 10.1016/j.pmpp.2024.102289 |
| spellingShingle | Hamid, Sathyapriya Chai, Ling Ho Abdullah, Siti Nor Akmar Ti, Eng Leslie Low Nagappan, Jayanthi Mui, Yun Wong Characterisation and expression analyses of two putative cerato-platanin proteins isolated from Ganoderma boninense Pat. |
| title | Characterisation and expression analyses of two putative cerato-platanin proteins isolated from Ganoderma boninense Pat. |
| title_full | Characterisation and expression analyses of two putative cerato-platanin proteins isolated from Ganoderma boninense Pat. |
| title_fullStr | Characterisation and expression analyses of two putative cerato-platanin proteins isolated from Ganoderma boninense Pat. |
| title_full_unstemmed | Characterisation and expression analyses of two putative cerato-platanin proteins isolated from Ganoderma boninense Pat. |
| title_short | Characterisation and expression analyses of two putative cerato-platanin proteins isolated from Ganoderma boninense Pat. |
| title_sort | characterisation and expression analyses of two putative cerato-platanin proteins isolated from ganoderma boninense pat. |
| url | http://psasir.upm.edu.my/id/eprint/120098/ http://psasir.upm.edu.my/id/eprint/120098/ http://psasir.upm.edu.my/id/eprint/120098/ http://psasir.upm.edu.my/id/eprint/120098/1/120098.pdf |