Optimization, physicochemical stability and in vivo study of alginate-chitosan composites as nanocarriers for low molecular weight angiotensin I-converting enzyme (ACE)-inhibitory peptide
Chitosan and alginate, are non-toxic and biodegradable polymers used to enhance the stability of biotherapeutics by loading them into nanocarriers. In this study, the stone fish-derived low molecular weight peptide (Ala-Leu-Gly-Pro-Gln-Phe-Tyr), exhibited an in vitro ACE-inhibitory activity of 94.43...
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| Format: | Article |
| Language: | English |
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Taiwan Food and Drug Administration
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120015/ http://psasir.upm.edu.my/id/eprint/120015/1/120015.pdf |
| _version_ | 1848868099372089344 |
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| author | Auwal, Shehu Muhammad Ghanisma, Siti Balqis Muhammad Saari, Nazamid |
| author_facet | Auwal, Shehu Muhammad Ghanisma, Siti Balqis Muhammad Saari, Nazamid |
| author_sort | Auwal, Shehu Muhammad |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Chitosan and alginate, are non-toxic and biodegradable polymers used to enhance the stability of biotherapeutics by loading them into nanocarriers. In this study, the stone fish-derived low molecular weight peptide (Ala-Leu-Gly-Pro-Gln-Phe-Tyr), exhibited an in vitro ACE-inhibitory activity of 94.43 ± 2.05% and an IC50 of 0.012 ± 0.001 mM. The peptide was encapsulated via ionic gelation with alginate followed by polyelectrolyte complexation with chitosan. The resulting ACE-inhibitory peptide-loaded alginate-chitosan nanoparticles (ACE-I-ALG-CS NPs) were optimized to achieve small particle size (212.60 nm) and high encapsulation efficiency (EE, 74.48%). This was based on an optimum chitosan concentration (0.420%w/v), homogenization speed (6000 rpm), and homogenization time (30 min) using Box Behnken experimental design (BBED). Characterization of the ACE-I-ALG-CS NPs revealed a spherical, monodispersed morphology with high physicochemical stability during storage at 2°C, 7°C, and 12°C for 12 weeks. Moreover, the in vivo study conducted on spontaneously hypertensive rats (SHRs) demonstrated a significantly higher (p < 0.05) systolic blood pressure (SBP)-lowering effect of the ACE-I-ALG-CS NPs compared to captopril and unencapsulated peptide. Hence, alginate and chitosan can be used as biocompatible coating materials to enhance the stability and in vivo anti-hypertensive effect of Ala-Leu-Gly-Pro-Gln-Phe-Tyr through encapsulation, thereby making it potentially valuable for various applications in pharmaceuticals and food industry. |
| first_indexed | 2025-11-15T14:47:00Z |
| format | Article |
| id | upm-120015 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:47:00Z |
| publishDate | 2024 |
| publisher | Taiwan Food and Drug Administration |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1200152025-10-28T05:36:44Z http://psasir.upm.edu.my/id/eprint/120015/ Optimization, physicochemical stability and in vivo study of alginate-chitosan composites as nanocarriers for low molecular weight angiotensin I-converting enzyme (ACE)-inhibitory peptide Auwal, Shehu Muhammad Ghanisma, Siti Balqis Muhammad Saari, Nazamid Chitosan and alginate, are non-toxic and biodegradable polymers used to enhance the stability of biotherapeutics by loading them into nanocarriers. In this study, the stone fish-derived low molecular weight peptide (Ala-Leu-Gly-Pro-Gln-Phe-Tyr), exhibited an in vitro ACE-inhibitory activity of 94.43 ± 2.05% and an IC50 of 0.012 ± 0.001 mM. The peptide was encapsulated via ionic gelation with alginate followed by polyelectrolyte complexation with chitosan. The resulting ACE-inhibitory peptide-loaded alginate-chitosan nanoparticles (ACE-I-ALG-CS NPs) were optimized to achieve small particle size (212.60 nm) and high encapsulation efficiency (EE, 74.48%). This was based on an optimum chitosan concentration (0.420%w/v), homogenization speed (6000 rpm), and homogenization time (30 min) using Box Behnken experimental design (BBED). Characterization of the ACE-I-ALG-CS NPs revealed a spherical, monodispersed morphology with high physicochemical stability during storage at 2°C, 7°C, and 12°C for 12 weeks. Moreover, the in vivo study conducted on spontaneously hypertensive rats (SHRs) demonstrated a significantly higher (p < 0.05) systolic blood pressure (SBP)-lowering effect of the ACE-I-ALG-CS NPs compared to captopril and unencapsulated peptide. Hence, alginate and chitosan can be used as biocompatible coating materials to enhance the stability and in vivo anti-hypertensive effect of Ala-Leu-Gly-Pro-Gln-Phe-Tyr through encapsulation, thereby making it potentially valuable for various applications in pharmaceuticals and food industry. Taiwan Food and Drug Administration 2024 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/120015/1/120015.pdf Auwal, Shehu Muhammad and Ghanisma, Siti Balqis Muhammad and Saari, Nazamid (2024) Optimization, physicochemical stability and in vivo study of alginate-chitosan composites as nanocarriers for low molecular weight angiotensin I-converting enzyme (ACE)-inhibitory peptide. Journal of Food and Drug Analysis, 32 (3). art. no. 8. pp. 358-370. ISSN 1021-9498; eISSN: 2224-6614 https://www.jfda-online.com/journal/vol32/iss3/8/ 10.38212/2224-6614.3522 |
| spellingShingle | Auwal, Shehu Muhammad Ghanisma, Siti Balqis Muhammad Saari, Nazamid Optimization, physicochemical stability and in vivo study of alginate-chitosan composites as nanocarriers for low molecular weight angiotensin I-converting enzyme (ACE)-inhibitory peptide |
| title | Optimization, physicochemical stability and in vivo study of alginate-chitosan composites as nanocarriers for low molecular weight angiotensin I-converting enzyme (ACE)-inhibitory peptide |
| title_full | Optimization, physicochemical stability and in vivo study of alginate-chitosan composites as nanocarriers for low molecular weight angiotensin I-converting enzyme (ACE)-inhibitory peptide |
| title_fullStr | Optimization, physicochemical stability and in vivo study of alginate-chitosan composites as nanocarriers for low molecular weight angiotensin I-converting enzyme (ACE)-inhibitory peptide |
| title_full_unstemmed | Optimization, physicochemical stability and in vivo study of alginate-chitosan composites as nanocarriers for low molecular weight angiotensin I-converting enzyme (ACE)-inhibitory peptide |
| title_short | Optimization, physicochemical stability and in vivo study of alginate-chitosan composites as nanocarriers for low molecular weight angiotensin I-converting enzyme (ACE)-inhibitory peptide |
| title_sort | optimization, physicochemical stability and in vivo study of alginate-chitosan composites as nanocarriers for low molecular weight angiotensin i-converting enzyme (ace)-inhibitory peptide |
| url | http://psasir.upm.edu.my/id/eprint/120015/ http://psasir.upm.edu.my/id/eprint/120015/ http://psasir.upm.edu.my/id/eprint/120015/ http://psasir.upm.edu.my/id/eprint/120015/1/120015.pdf |