Sequence, structure and biophysical characterization of CsoR-like hypothetical protein from Geobacillus zalihae strain T1
Aims: To date, nine classes of copper regulation mechanisms have been discovered in bacteria and CsoR regulator protein is the most recent among them. Only a few have been structurally and functionally characterized. The present study was aimed to isolate and characterize the sequence, structure and...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2024
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/119836/ http://psasir.upm.edu.my/id/eprint/119836/1/119836.pdf |
| _version_ | 1848868064451362816 |
|---|---|
| author | Musa, Nasihah Mangavelu, Ashwaani Au, Shaw Xian Mohd Padzil, Azyyati Kriznik, Alexandre Mohammad Latif, Muhammad Alif Jonet, Mohd Anuar Ahmad Rodzli, Nazahiyah Leow, Thean Chor Mohamad Ali, Mohd Shukuri Raja Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar M. Normi, Yahaya |
| author_facet | Musa, Nasihah Mangavelu, Ashwaani Au, Shaw Xian Mohd Padzil, Azyyati Kriznik, Alexandre Mohammad Latif, Muhammad Alif Jonet, Mohd Anuar Ahmad Rodzli, Nazahiyah Leow, Thean Chor Mohamad Ali, Mohd Shukuri Raja Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar M. Normi, Yahaya |
| author_sort | Musa, Nasihah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aims: To date, nine classes of copper regulation mechanisms have been discovered in bacteria and CsoR regulator protein is the most recent among them. Only a few have been structurally and functionally characterized. The present study was aimed to isolate and characterize the sequence, structure and biochemical properties of CsoRGz-like hypothetical protein (HP) to be potentially used as a copper sensor protein. Methodology and results: A scan of the complete genome of a Geobacillus zalihae strain T1 revealed the presence of CsoR-like (CsoRGz) HP, which contains CsoR-like_DUF156 domain and highly conserved Cys-His-Cys residues essential for copper binding. It only shares moderate sequence identity with structurally characterized CsoR proteins. CsoRGz-like HP was subjected to sequence analyses to identify important domains, motifs and residues, while circular dichroism and X-ray crystallography were used to determine its secondary and tertiary structures. CsoRGz appears to be a dimer comprising mainly α-helices, with putative, conserved metal-binding ligands, Cys46-His71-Cys75, located on the α2 helix of the protein. Biophysical characterization of CsoRGz using UV/Vis and fluorescence spectrophotometry confirmed its interaction with Cu(I). Docking of Cu(I) to the dimeric structure of CsoRGz showed that Cu(I) could be coordinated by the above metal-binding residues, further stabilized by the hydrophobic core at the metal-binding site. Conclusion, significance and impact of study: The findings in this study suggest that CsoRGz-like HP might be a novel CsoR protein. This adds to the breadth and numbers of possible CsoR proteins, particularly uncharacterized ones, existing in the pool of hypothetical proteins, to be further characterized and compared across bacterial taxa. |
| first_indexed | 2025-11-15T14:46:27Z |
| format | Article |
| id | upm-119836 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:46:27Z |
| publishDate | 2024 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1198362025-09-19T08:14:04Z http://psasir.upm.edu.my/id/eprint/119836/ Sequence, structure and biophysical characterization of CsoR-like hypothetical protein from Geobacillus zalihae strain T1 Musa, Nasihah Mangavelu, Ashwaani Au, Shaw Xian Mohd Padzil, Azyyati Kriznik, Alexandre Mohammad Latif, Muhammad Alif Jonet, Mohd Anuar Ahmad Rodzli, Nazahiyah Leow, Thean Chor Mohamad Ali, Mohd Shukuri Raja Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar M. Normi, Yahaya Aims: To date, nine classes of copper regulation mechanisms have been discovered in bacteria and CsoR regulator protein is the most recent among them. Only a few have been structurally and functionally characterized. The present study was aimed to isolate and characterize the sequence, structure and biochemical properties of CsoRGz-like hypothetical protein (HP) to be potentially used as a copper sensor protein. Methodology and results: A scan of the complete genome of a Geobacillus zalihae strain T1 revealed the presence of CsoR-like (CsoRGz) HP, which contains CsoR-like_DUF156 domain and highly conserved Cys-His-Cys residues essential for copper binding. It only shares moderate sequence identity with structurally characterized CsoR proteins. CsoRGz-like HP was subjected to sequence analyses to identify important domains, motifs and residues, while circular dichroism and X-ray crystallography were used to determine its secondary and tertiary structures. CsoRGz appears to be a dimer comprising mainly α-helices, with putative, conserved metal-binding ligands, Cys46-His71-Cys75, located on the α2 helix of the protein. Biophysical characterization of CsoRGz using UV/Vis and fluorescence spectrophotometry confirmed its interaction with Cu(I). Docking of Cu(I) to the dimeric structure of CsoRGz showed that Cu(I) could be coordinated by the above metal-binding residues, further stabilized by the hydrophobic core at the metal-binding site. Conclusion, significance and impact of study: The findings in this study suggest that CsoRGz-like HP might be a novel CsoR protein. This adds to the breadth and numbers of possible CsoR proteins, particularly uncharacterized ones, existing in the pool of hypothetical proteins, to be further characterized and compared across bacterial taxa. Universiti Sains Malaysia 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/119836/1/119836.pdf Musa, Nasihah and Mangavelu, Ashwaani and Au, Shaw Xian and Mohd Padzil, Azyyati and Kriznik, Alexandre and Mohammad Latif, Muhammad Alif and Jonet, Mohd Anuar and Ahmad Rodzli, Nazahiyah and Leow, Thean Chor and Mohamad Ali, Mohd Shukuri and Raja Abd Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and M. Normi, Yahaya (2024) Sequence, structure and biophysical characterization of CsoR-like hypothetical protein from Geobacillus zalihae strain T1. Malaysian Journal of Microbiology, 20 (4). pp. 417-429. ISSN 1823-8262; eISSN: 2231-7538 https://mjm.usm.my/index.php?r=cms/entry/view&id=2846&slug=Sequence%2C-structure-and-biophysical-characterization-of-CsoR-like-hypothetical-protein-from-Geobacillus-zalihae-strain-T1 10.21161/mjm.230265 |
| spellingShingle | Musa, Nasihah Mangavelu, Ashwaani Au, Shaw Xian Mohd Padzil, Azyyati Kriznik, Alexandre Mohammad Latif, Muhammad Alif Jonet, Mohd Anuar Ahmad Rodzli, Nazahiyah Leow, Thean Chor Mohamad Ali, Mohd Shukuri Raja Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar M. Normi, Yahaya Sequence, structure and biophysical characterization of CsoR-like hypothetical protein from Geobacillus zalihae strain T1 |
| title | Sequence, structure and biophysical characterization of CsoR-like hypothetical protein from Geobacillus zalihae strain T1 |
| title_full | Sequence, structure and biophysical characterization of CsoR-like hypothetical protein from Geobacillus zalihae strain T1 |
| title_fullStr | Sequence, structure and biophysical characterization of CsoR-like hypothetical protein from Geobacillus zalihae strain T1 |
| title_full_unstemmed | Sequence, structure and biophysical characterization of CsoR-like hypothetical protein from Geobacillus zalihae strain T1 |
| title_short | Sequence, structure and biophysical characterization of CsoR-like hypothetical protein from Geobacillus zalihae strain T1 |
| title_sort | sequence, structure and biophysical characterization of csor-like hypothetical protein from geobacillus zalihae strain t1 |
| url | http://psasir.upm.edu.my/id/eprint/119836/ http://psasir.upm.edu.my/id/eprint/119836/ http://psasir.upm.edu.my/id/eprint/119836/ http://psasir.upm.edu.my/id/eprint/119836/1/119836.pdf |