Engineering kratom-based nanoformulation for wound healing
Nanostructured lipid carriers (NLC) have evolved as effective vehicles for improving the bioavailability of poorly water-soluble drugs as well as protecting them from degradation and metabolism. The present study investigated the preformulation and its impact on different liquid lipids and solid...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/119670/ http://psasir.upm.edu.my/id/eprint/119670/1/119670.pdf |
| _version_ | 1848868033896906752 |
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| author | Ashari, Siti Efliza Syed Azhar, Sharifah Nurfadhlin Afifah Hasan, Masriana Wilkinson, Holly |
| author_facet | Ashari, Siti Efliza Syed Azhar, Sharifah Nurfadhlin Afifah Hasan, Masriana Wilkinson, Holly |
| author_sort | Ashari, Siti Efliza |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Nanostructured lipid carriers (NLC) have evolved as effective vehicles for improving the
bioavailability of poorly water-soluble drugs as well as protecting them from degradation and
metabolism. The present study investigated the preformulation and its impact on different liquid
lipids and solid lipids with diverse chemical structures and hydrophilic-lipophilic balance (HLB)
for the critical quality attributes of Mitragyna speciosa leaves extract (MS)- based nanostructured
lipid carriers suitable for drug delivery system. The presence of bioactive alkaloids (mitragynine,
7-hydroxymitragynine, speciogynine), flavonoids (quercetin, apigenin, kaempferol), saponins
(daucosterol, quinovic acid 3-o-beta D-quinovopyranoside, 1-o-feruloyl-beta D-glucose), and
other bioactive phytochemicals (chlorogenic acid, umbelliferone, ursolic acid) in MS leaves
extract was detected using LCMS/MS analysis to support its medicinal properties. The total
phenolic content, total flavonoid content, and DPPH assay of MS were 90.88 ± 0.30 GAE mg/g,
50.43 ± 0.47 QE mg/g, and IC50 at 0.0397 ± 0.0035 mg/mL, respectively. The screening
preparation of NLC requires the selection of liquid lipid (oil), solid lipid, and surfactant. Based on
the solubility test, the maximum solubility of MS leaves extract in oleic acid (liquid lipid) and
compritol ATO 888 (solid lipid) were 2.859 ± 0.010% and 53.02 ± 0.39% respectively at ratio
70:30. Tween 80 was selected as the main surfactant for the preparation of NLC due to its highest
emulsification efficiency (51.53%). The antimicrobial study showed that MS leaves extract gives
antimicrobial effect towards Streptococcus aureus bacteria. The preformulation information
collected offers a better, efficient, and cost-effective choice for further formulation development
of MS leaves extract in drug delivery systems. |
| first_indexed | 2025-11-15T14:45:58Z |
| format | Conference or Workshop Item |
| id | upm-119670 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:45:58Z |
| publishDate | 2025 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1196702025-09-04T03:24:00Z http://psasir.upm.edu.my/id/eprint/119670/ Engineering kratom-based nanoformulation for wound healing Ashari, Siti Efliza Syed Azhar, Sharifah Nurfadhlin Afifah Hasan, Masriana Wilkinson, Holly Nanostructured lipid carriers (NLC) have evolved as effective vehicles for improving the bioavailability of poorly water-soluble drugs as well as protecting them from degradation and metabolism. The present study investigated the preformulation and its impact on different liquid lipids and solid lipids with diverse chemical structures and hydrophilic-lipophilic balance (HLB) for the critical quality attributes of Mitragyna speciosa leaves extract (MS)- based nanostructured lipid carriers suitable for drug delivery system. The presence of bioactive alkaloids (mitragynine, 7-hydroxymitragynine, speciogynine), flavonoids (quercetin, apigenin, kaempferol), saponins (daucosterol, quinovic acid 3-o-beta D-quinovopyranoside, 1-o-feruloyl-beta D-glucose), and other bioactive phytochemicals (chlorogenic acid, umbelliferone, ursolic acid) in MS leaves extract was detected using LCMS/MS analysis to support its medicinal properties. The total phenolic content, total flavonoid content, and DPPH assay of MS were 90.88 ± 0.30 GAE mg/g, 50.43 ± 0.47 QE mg/g, and IC50 at 0.0397 ± 0.0035 mg/mL, respectively. The screening preparation of NLC requires the selection of liquid lipid (oil), solid lipid, and surfactant. Based on the solubility test, the maximum solubility of MS leaves extract in oleic acid (liquid lipid) and compritol ATO 888 (solid lipid) were 2.859 ± 0.010% and 53.02 ± 0.39% respectively at ratio 70:30. Tween 80 was selected as the main surfactant for the preparation of NLC due to its highest emulsification efficiency (51.53%). The antimicrobial study showed that MS leaves extract gives antimicrobial effect towards Streptococcus aureus bacteria. The preformulation information collected offers a better, efficient, and cost-effective choice for further formulation development of MS leaves extract in drug delivery systems. 2025 Conference or Workshop Item NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/119670/1/119670.pdf Ashari, Siti Efliza and Syed Azhar, Sharifah Nurfadhlin Afifah and Hasan, Masriana and Wilkinson, Holly (2025) Engineering kratom-based nanoformulation for wound healing. In: 5th International Conference on Science and Engineering of Materials, 4-7 Jan. 2025, Sharda University, India. . |
| spellingShingle | Ashari, Siti Efliza Syed Azhar, Sharifah Nurfadhlin Afifah Hasan, Masriana Wilkinson, Holly Engineering kratom-based nanoformulation for wound healing |
| title | Engineering kratom-based nanoformulation for wound healing |
| title_full | Engineering kratom-based nanoformulation for wound healing |
| title_fullStr | Engineering kratom-based nanoformulation for wound healing |
| title_full_unstemmed | Engineering kratom-based nanoformulation for wound healing |
| title_short | Engineering kratom-based nanoformulation for wound healing |
| title_sort | engineering kratom-based nanoformulation for wound healing |
| url | http://psasir.upm.edu.my/id/eprint/119670/ http://psasir.upm.edu.my/id/eprint/119670/1/119670.pdf |