Mutagenic potential of fried and boiled “Keropok lekor”: a study using the Ames test
“Keropok lekor”, a traditional Malaysian fish sausage, is widely consumed, yet its mutagenic potential has not been thoroughly investigated. Objective: This study evaluated the mutagenic activity of keropok lekor samples. Methodology: Samples were analysed using the Ames test with Salmonella typhimu...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Environmental and Occupational Health Society
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/119636/ http://psasir.upm.edu.my/id/eprint/119636/1/119636.pdf |
| _version_ | 1848868025405538304 |
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| author | Abdullah, Rozaini Shafie, Siti Raihanah Engku Puteri Nurfaranajieha |
| author_facet | Abdullah, Rozaini Shafie, Siti Raihanah Engku Puteri Nurfaranajieha |
| author_sort | Abdullah, Rozaini |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | “Keropok lekor”, a traditional Malaysian fish sausage, is widely consumed, yet its mutagenic potential has not been thoroughly investigated. Objective: This study evaluated the mutagenic activity of keropok lekor samples. Methodology: Samples were analysed using the Ames test with Salmonella typhimurium strains TA98 and TA100, with and without S9 metabolic activation. Boiled “keropok lekor” and fried samples variants were tested at concentrations of 1.0, 2.0, and 4.0 mg/mL. Results: The fried samples demonstrated significant mutagenicity in the absence of metabolic activation, with revertant colony counts exceeding the two-fold threshold of solvent controls at higher concentrations. In contrast, the boiled samples showed lower mutagenic activity, suggesting that frying may increase mutagenicity. Furthermore, while the addition of S9 metabolic activation reduced mutagenic responses, a dose-response relationship was noted in both variants, indicating a concentration-dependent effect. These findings highlight the potential health risks associated with frequent consumption of fried “keropok lekor” and emphasize the need to evaluate traditional foods for genotoxic risks. |
| first_indexed | 2025-11-15T14:45:50Z |
| format | Article |
| id | upm-119636 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:45:50Z |
| publishDate | 2024 |
| publisher | Environmental and Occupational Health Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1196362025-09-02T03:44:33Z http://psasir.upm.edu.my/id/eprint/119636/ Mutagenic potential of fried and boiled “Keropok lekor”: a study using the Ames test Abdullah, Rozaini Shafie, Siti Raihanah Engku Puteri Nurfaranajieha “Keropok lekor”, a traditional Malaysian fish sausage, is widely consumed, yet its mutagenic potential has not been thoroughly investigated. Objective: This study evaluated the mutagenic activity of keropok lekor samples. Methodology: Samples were analysed using the Ames test with Salmonella typhimurium strains TA98 and TA100, with and without S9 metabolic activation. Boiled “keropok lekor” and fried samples variants were tested at concentrations of 1.0, 2.0, and 4.0 mg/mL. Results: The fried samples demonstrated significant mutagenicity in the absence of metabolic activation, with revertant colony counts exceeding the two-fold threshold of solvent controls at higher concentrations. In contrast, the boiled samples showed lower mutagenic activity, suggesting that frying may increase mutagenicity. Furthermore, while the addition of S9 metabolic activation reduced mutagenic responses, a dose-response relationship was noted in both variants, indicating a concentration-dependent effect. These findings highlight the potential health risks associated with frequent consumption of fried “keropok lekor” and emphasize the need to evaluate traditional foods for genotoxic risks. Environmental and Occupational Health Society 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/119636/1/119636.pdf Abdullah, Rozaini and Shafie, Siti Raihanah and Engku Puteri Nurfaranajieha (2024) Mutagenic potential of fried and boiled “Keropok lekor”: a study using the Ames test. Asia Pacific Environmental and Occupational Health Journal, 10 (7). pp. 1-13. ISSN 2462-2214 http://apeohjournal.org/index.php/v/article/view/199 |
| spellingShingle | Abdullah, Rozaini Shafie, Siti Raihanah Engku Puteri Nurfaranajieha Mutagenic potential of fried and boiled “Keropok lekor”: a study using the Ames test |
| title | Mutagenic potential of fried and boiled “Keropok lekor”: a study using the Ames test |
| title_full | Mutagenic potential of fried and boiled “Keropok lekor”: a study using the Ames test |
| title_fullStr | Mutagenic potential of fried and boiled “Keropok lekor”: a study using the Ames test |
| title_full_unstemmed | Mutagenic potential of fried and boiled “Keropok lekor”: a study using the Ames test |
| title_short | Mutagenic potential of fried and boiled “Keropok lekor”: a study using the Ames test |
| title_sort | mutagenic potential of fried and boiled “keropok lekor”: a study using the ames test |
| url | http://psasir.upm.edu.my/id/eprint/119636/ http://psasir.upm.edu.my/id/eprint/119636/ http://psasir.upm.edu.my/id/eprint/119636/1/119636.pdf |