Electrochemical aptasensor for 2-amino-2-thiazoline-4- 2 carboxylic acid (ATCA), a metabolite for cyanide 3 poisoning
An alternative biomarker for assessing the cyanide levels in postmortem materials is crucial for investigating acute cyanide intoxication. Herein, an aptamer–ligand biorecognition system with high specificity was developed to detect acute cyanide poisoning via its secondary metabolite, 2-amino-2-thi...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/119435/ http://psasir.upm.edu.my/id/eprint/119435/1/119435.pdf |
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| author | Yusof, Nor Azah Mohamad Anas Khan, Hairunnisa Yee, Yu Choo Alang Ahmad, Shahrul Ainliah Raston, Nurul Hanunahmad Abd Rahman, Siti Fatimah |
| author_facet | Yusof, Nor Azah Mohamad Anas Khan, Hairunnisa Yee, Yu Choo Alang Ahmad, Shahrul Ainliah Raston, Nurul Hanunahmad Abd Rahman, Siti Fatimah |
| author_sort | Yusof, Nor Azah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An alternative biomarker for assessing the cyanide levels in postmortem materials is crucial for investigating acute cyanide intoxication. Herein, an aptamer–ligand biorecognition system with high specificity was developed to detect acute cyanide poisoning via its secondary metabolite, 2-amino-2-thiazoline-4-carboxylic acid (ATCA). Potential aptamers were screened from a random library of 66-base single-stranded DNA using GO-SELEX, with individual aptamers being identified through single-stranded DNA sequencing. Molecular docking was employed to predict the affinity of these aptamers toward ATCA and selected counter-targets; these predictions were confirmed using thermodynamic analysis with an isothermal titration calorimeter. Owing to its label-free biomolecular binding interactions, Apt46 exhibited the highest affinity against ATCA and notable selectivity against structurally similar counter-targets. Thus, an amino-tagged Apt46 binding aptamer was attached to a carbon electrode modified with EDC–NHS-activated graphene oxide. The binding of Apt46 to ATCA was quantified by measuring current changes using differential pulse voltammetry. The aptasensor achieved a detection limit of 0.05 µg/mL and demonstrated suitability for detecting ATCA across various biological matrices, with the high recovery percentages ranging from 92.29 to 114.22%. Overall, the proposed ATCA aptasensor is promising for identifying ATCA metabolites in cases of acute cyanide expose. |
| first_indexed | 2025-11-15T14:44:52Z |
| format | Article |
| id | upm-119435 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:44:52Z |
| publishDate | 2024 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1194352025-08-22T03:47:01Z http://psasir.upm.edu.my/id/eprint/119435/ Electrochemical aptasensor for 2-amino-2-thiazoline-4- 2 carboxylic acid (ATCA), a metabolite for cyanide 3 poisoning Yusof, Nor Azah Mohamad Anas Khan, Hairunnisa Yee, Yu Choo Alang Ahmad, Shahrul Ainliah Raston, Nurul Hanunahmad Abd Rahman, Siti Fatimah An alternative biomarker for assessing the cyanide levels in postmortem materials is crucial for investigating acute cyanide intoxication. Herein, an aptamer–ligand biorecognition system with high specificity was developed to detect acute cyanide poisoning via its secondary metabolite, 2-amino-2-thiazoline-4-carboxylic acid (ATCA). Potential aptamers were screened from a random library of 66-base single-stranded DNA using GO-SELEX, with individual aptamers being identified through single-stranded DNA sequencing. Molecular docking was employed to predict the affinity of these aptamers toward ATCA and selected counter-targets; these predictions were confirmed using thermodynamic analysis with an isothermal titration calorimeter. Owing to its label-free biomolecular binding interactions, Apt46 exhibited the highest affinity against ATCA and notable selectivity against structurally similar counter-targets. Thus, an amino-tagged Apt46 binding aptamer was attached to a carbon electrode modified with EDC–NHS-activated graphene oxide. The binding of Apt46 to ATCA was quantified by measuring current changes using differential pulse voltammetry. The aptasensor achieved a detection limit of 0.05 µg/mL and demonstrated suitability for detecting ATCA across various biological matrices, with the high recovery percentages ranging from 92.29 to 114.22%. Overall, the proposed ATCA aptasensor is promising for identifying ATCA metabolites in cases of acute cyanide expose. Nature Publishing Group 2024-10-11 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/119435/1/119435.pdf Yusof, Nor Azah and Mohamad Anas Khan, Hairunnisa and Yee, Yu Choo and Alang Ahmad, Shahrul Ainliah and Raston, Nurul Hanunahmad and Abd Rahman, Siti Fatimah (2024) Electrochemical aptasensor for 2-amino-2-thiazoline-4- 2 carboxylic acid (ATCA), a metabolite for cyanide 3 poisoning. Scientific Reports, 14. art. no. 23859. pp. 1-15. ISSN 2045-2322 https://www.nature.com/articles/s41598-024-72503-y |
| spellingShingle | Yusof, Nor Azah Mohamad Anas Khan, Hairunnisa Yee, Yu Choo Alang Ahmad, Shahrul Ainliah Raston, Nurul Hanunahmad Abd Rahman, Siti Fatimah Electrochemical aptasensor for 2-amino-2-thiazoline-4- 2 carboxylic acid (ATCA), a metabolite for cyanide 3 poisoning |
| title | Electrochemical aptasensor for 2-amino-2-thiazoline-4- 2 carboxylic acid (ATCA), a metabolite for cyanide 3 poisoning |
| title_full | Electrochemical aptasensor for 2-amino-2-thiazoline-4- 2 carboxylic acid (ATCA), a metabolite for cyanide 3 poisoning |
| title_fullStr | Electrochemical aptasensor for 2-amino-2-thiazoline-4- 2 carboxylic acid (ATCA), a metabolite for cyanide 3 poisoning |
| title_full_unstemmed | Electrochemical aptasensor for 2-amino-2-thiazoline-4- 2 carboxylic acid (ATCA), a metabolite for cyanide 3 poisoning |
| title_short | Electrochemical aptasensor for 2-amino-2-thiazoline-4- 2 carboxylic acid (ATCA), a metabolite for cyanide 3 poisoning |
| title_sort | electrochemical aptasensor for 2-amino-2-thiazoline-4- 2 carboxylic acid (atca), a metabolite for cyanide 3 poisoning |
| url | http://psasir.upm.edu.my/id/eprint/119435/ http://psasir.upm.edu.my/id/eprint/119435/ http://psasir.upm.edu.my/id/eprint/119435/1/119435.pdf |